Validation in ASP.NET ensures that user-entered data meets defined formats and criteria. There are five validator controls that check for specific errors, like missing required fields or invalid data types. All validators inherit properties from the BaseValidator class and can be configured to display error messages and enable client-side validation. Regular expression validators check input against patterns. Validation controls can be grouped and their errors displayed in a summary for the user to easily see and correct mistakes. Manual validation is also possible by disabling the controls and writing custom validation logic.