산모 흡연 연구과제

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산모 흡연 연구과제

  1. 1. 임신 초기 임신부의흡연 노출 평가진단검사의학과곽호석
  2. 2. Smoking patterns during pregnancy평균 4주평균 7주
  3. 3. Quantitation of Metabolites of NNK afterCessation of Smokeless Tobacco UseTime post-cessation (days)SmokingNNAL t1/2: 10-18 days > cotinine t1/2 : 1days0.0%20.0%40.0%60.0%80.0%100.0%120.0%0 2 4 6 8 10 12 14 16 18 20Cocncentration(%)DayCotinineNNAL
  4. 4. The metabolic activation andinactivation of NNK* 11β-HSD: 11β-hydroxysteroid dehydrogenaseSteroid hormonesDrug: diureticsDrug: block 11β-HSDDrugDrug: flavonoidFlavanoneSimilar structure: hormones
  5. 5. Total NNAL after exposure to sidestream smoke (4hr)
  6. 6. Urine Concentrations of a Tobacco-Specific NitrosamineCarcinogen in the U.S. Population from SecondhandSmoke Exposure
  7. 7. NNAL concentration according to cigarette brand미국 벡터(Vector)사는 지난 2001년 발암성 물질을 줄인 담배‘OMNI’를 선보인 데 이어, 2003년에는 저니코틴 담배 ‘QUEST’를출시했다. 이 담배는 기존 담배와 비교해 니코틴 함량을 97% 낮춘GM(유전자조작)담배로 제조됐다. 그러나 향과 맛 등의 주요 특징들은 기존 담배와 거의 동일한 것으로 전해졌다.A Vector Tobacco website claimed Omni produceda 53% reduction in exposure to NNK.
  8. 8. NNAL 분석
  9. 9. Comparison of analytical methodAuthor Urine vol. LOQ(pg/mL)Analyticalconcentration of LOQSample preparationHou Hongwei etal. (2012)5 mL 0.99 50 pg/mLEnzymereaction(overnight),MIPShowket H.Bhat et al.(2011) 0.25 mL 20 50 pg/mLEnzyme reaction(20h),derivatization(12h),SPEDominiqueKavvadias et al.(2009)6 mL 5 300 pg/mLEnzyme reaction(14h),MIP, SPEYang Xia et al.(2005) 5 mL 1.7 425 pg/mLEnzyme reaction(24h),MIP
  10. 10.  Sample preparation• Enzyme reaction: beta-glucuronidase (overnight)• NNAL extraction: MIP(molecular imprinting polymer) column (2hr)• Measurement of NNAL: LC-MS/MS (6min/sample)
  11. 11. Chromatograms of the ion transition for NNAL and itsdeuterated internal standard in urine sample of a smoker5 ng/mL 500 ng/mLNNAL-d3 NNAL-d3NNAL NNALUrine: 1mL (20cigarette/day, male)Non-enzyme digestion, MIP column
  12. 12. Microwave-Assisted Enzyme-Catalyzed Reactions
  13. 13. Metabolism of TSANNNA has been described as a veryreactive aldehyde which has neverbeen quantified in tobacco or tobaccosmoke. There has been considerableinterest in recent yearsin the chemical composition ofsmokeless tobacco products basedaround health concerns associatedwith their use
  14. 14. 경청해 주셔서 감사합니다.

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