1. 임신 초기 임신부의
흡연 노출 평가
진단검사의학과
곽호석

2. Smoking patterns during pregnancy
평균 4주
평균 7주

3. Quantitation of Metabolites of NNK after
Cessation of Smokeless Tobacco Use
Time post-cessation (days)
Smoking
NNAL t1/2: 10-18 days > cotinine t1/2 : 1days
0.0%
20.0%
40.0%
60.0%
80.0%
100.0%
120.0%
0 2 4 6 8 10 12 14 16 18 20Cocncentration(%)
Day
Cotinine
NNAL

4. The metabolic activation and
inactivation of NNK
* 11β-HSD: 11β-hydroxysteroid dehydrogenase
Steroid hormones
Drug: diuretics
Drug: block 11β-HSD
Drug
Drug: flavonoid
Flavanone
Similar structure: hormones

5. Total NNAL after exposure to sidestream smoke (4hr)

6. Urine Concentrations of a Tobacco-Specific Nitrosamine
Carcinogen in the U.S. Population from Secondhand
Smoke Exposure

7. NNAL concentration according to cigarette brand
미국 벡터(Vector)사는 지난 2001년 발암성 물질을 줄인 담배
‘OMNI’를 선보인 데 이어, 2003년에는 저니코틴 담배 ‘QUEST’를
출시했다. 이 담배는 기존 담배와 비교해 니코틴 함량을 97% 낮춘
GM(유전자조작)담배로 제조됐다. 그러나 향과 맛 등의 주요 특징
들은 기존 담배와 거의 동일한 것으로 전해졌다.
A Vector Tobacco website claimed Omni produced
a 53% reduction in exposure to NNK.

8. NNAL 분석

9. Comparison of analytical method
Author Urine vol. LOQ
(pg/mL)
Analytical
concentration of LOQ
Sample preparation
Hou Hongwei et
al. (2012)
5 mL 0.99 50 pg/mL
Enzyme
reaction(overnight),
MIP
Showket H.
Bhat et al.
(2011) 0.25 mL 20 50 pg/mL
Enzyme reaction(20h),
derivatization(12h),
SPE
Dominique
Kavvadias et al.
(2009)
6 mL 5 300 pg/mL
Enzyme reaction(14h),
MIP, SPE
Yang Xia et al.
(2005) 5 mL 1.7 425 pg/mL
Enzyme reaction(24h),
MIP

10.  Sample preparation
• Enzyme reaction: beta-glucuronidase (overnight)
• NNAL extraction: MIP(molecular imprinting polymer) column (2hr)
• Measurement of NNAL: LC-MS/MS (6min/sample)

12. Chromatograms of the ion transition for NNAL and its
deuterated internal standard in urine sample of a smoker
5 ng/mL 500 ng/mL
NNAL-d3 NNAL-d3
NNAL NNAL
Urine: 1mL (20cigarette/day, male)
Non-enzyme digestion, MIP column

13. Microwave-Assisted Enzyme-
Catalyzed Reactions

14. Metabolism of TSAN
NNA has been described as a very
reactive aldehyde which has never
been quantified in tobacco or tobacco
smoke. There has been considerable
interest in recent years
in the chemical composition of
smokeless tobacco products based
around health concerns associated
with their use

15. 경청해 주셔서 감사합니다.