ChromaDex provides an update on its $62 million agreement with 5LINX, a network marketing company, to supply ingredients for new health products. 5LINX plans to launch products containing ChromaDex's nicotinamide riboside (NR) and PURENERGY ingredients at its May event. ChromaDex will attend and expects to meet the first year minimum purchase requirements from 5LINX. The article provides background on NR and PURENERGY and their potential health benefits.

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ChromaDex Provides Update on $62 Million Ingredient Agreement with Network Marketing
Company 5LINX®
- 5LINX® To Launch New Product Suite Featuring ChromaDex's Patented NIAGEN™
Nicotinamide Riboside and PURENERGY® at Annual Event in May -
IRVINE, Calif., April 23, 2014 /PRNewswire/ -- ChromaDex Corp. (OTCQB: CDXC), an
innovative natural products company that provides proprietary, ingredients and science-based
solutions to the dietary supplement, food and beverage, animal health, cosmetic and
pharmaceutical industries, provided an update today on its previously announced $62 million,
four-year ingredient supply and brand licensing agreement with 5LINX®, one of the largest and
fastest-growing direct marketing companies in the world.
5LINX® plans on launching several new innovative health and wellness products that feature
ChromaDex's recently launched patented NIAGEN™ nicotinamide riboside (NR) and patented
PURENERGY® ingredients at its upcoming international event in Atlantic City, NJ. The event
will be held May 14 to 17 and is expected to be attended by thousands of 5LINX® sales
associates. ChromaDex's CEO and founder Frank L. Jaksch, Jr., will attend, and iconic
motivational speaker Tony Robbins will be among those addressing the attendees.
Craig Jerabeck, CEO of 5LINX® commented, "The health and wellness story behind NIAGEN™
nicotinamide riboside continues to grow, as numerous favorable studies and articles have been
published since this past January when we decided to be among the first to offer dietary
supplements featuring NIAGEN™. Our level of enthusiasm and anticipation continues to grow
as we count down the days to our annual event and the launch of our new line of products
featuring ChromaDex's patented ingredients."
Jaksch stated, "We are more convinced than ever that the network marketing channel is ideal for
the early development and adoption of health and wellness products featuring our patented
NIAGEN™ and PURENERGY® ingredients. Our interaction with the 5LINX® management
team has been outstanding. We will report in our 1st quarter results meaningful revenue related
to the 5LINX® deal, and early indications are that 5LINX® will likely easily exceed the first year
minimum ingredient purchase requirements set out in our agreement. I look forward to attending
their annual event in May in Atlantic City and to being part of the celebrated launch of their
products featuring our ingredients."
In January 2014, 5LINX® was granted marketing rights for ChromaDex's recently launched
patented NIAGEN™ nicotinamide riboside (NR) for use in dietary supplements exclusively in
the network marketing sales channel in the United States, Canada and the Philippines. Under the
terms of the agreement, 5LINX® must purchase from ChromaDex $2.1 million of NIAGEN™ in
2014, and an aggregate of $46 million of NIAGEN™ from 2015 through 2017. NIAGEN™ is
the first and only commercially available brand of NR, which is found naturally in trace amounts
in milk and other foods and is a more potent, no-flush version of niacin (vitamin B3). Published
research has shown that NR is perhaps the most potent precursor to NAD+ in the mitochondria

2. of animals. NAD+ is arguably the most important cellular co-factor for improvement of
mitochondrial performance and energy metabolism.
5LINX® was also granted marketing rights for ChromaDex's patented PURENERGY® ingredient
for use in dietary supplements, with select exclusivity in the network marketing sales channel in
the United States, Canada and Philippines. Under the exclusive network marketing channel
agreement, 5LINX® must purchase from ChromaDex $520,000 of PURENERGY® in 2014 and an
aggregate of $14 million of PURENERGY® from 2015 through 2017. ChromaDex's
PURENERGY® innovative caffeine ingredient is a patented co-crystal combination of caffeine
and ChromaDex's pTeroPure® pterostilbene, a potent anti-oxidant found in blueberries. The first
human study of PURENERGY® demonstrated PURENERGY® delivers 30 percent more caffeine,
stays in the blood stream longer, and is absorbed more slowly than ordinary caffeine. With
PURENERGY® formulators of energy products may have the ability to reduce the total amount
of caffeine in their products by as much as 50% without sacrificing consumers' expectations
from such products.
About Nicotinamide Riboside (NR):
Sometimes referred to as the "Miracle Molecule" or "Hidden Vitamin," NR is found naturally in
trace amounts in milk and other foods and is a more potent, no-flush vitamin B3 (Niacin)
derivative.
Published research has shown that NR is perhaps the most effective NAD+ booster, an essential
metabolite found in all cells. NAD+ is arguably the most important cellular co-factor for
improvement of mitochondrial performance and energy metabolism. Mitochondria are the
powerhouse of the cell where macronutrients are converted to energy the cell can use.
Mitochondria also play an important part in the aging process. It is hoped that by stimulating
mitochondrial function with the NR molecule, scientists may see increased longevity as well as
other health improvements. Researchers worldwide continue seminal discoveries characterizing
the unique properties of NR in a wide range of health benefits. These include: increasing
mitochondrial health, increasing muscle endurance, neuroprotection, sirtuin activation, protection
against weight gain on high fat diet, protection against oxidative stress, improvement of blood
glucose and insulin sensitivity to maintain blood sugar levels already within the normal range.
Findings from a recent study by Weill Cornell Medical College and the Ecole Polytechnique
Federale de Lausanne, Switzerland researchers showed mice on a high-fat diet that were fed NR
gained 60 percent less weight than mice eating the same high-fat diet without NR. Moreover,
unlike the mice that were not fed NR, none of the NR-treated mice had indications that they were
developing diabetes and they had improved energy and lower cholesterol levels, all without side
effects. The Swiss researchers were quoted as saying the effects of NR on metabolism "are
nothing short of astonishing."
A study by researchers from Harvard Medical School in conjunction with the National Institute
on Aging was published in December 2013 in CELL demonstrated that mitochondrial
dysfunction (a hallmark of aging) in aging mice is due to a disruption in Sirtuin1-dependent
nuclear-mitochondrial communication. They further show that a reduction in NAD+ levels is

3. responsible for this disruption. Excitingly, they also show that this mitochondrial dysfunction is
readily reversible by administration of a NAD+ precursor. The authors show that "1 week of
treatment with a compound that boosts NAD+ levels is sufficient to restore the mitochondrial
homeostasis and key biochemical markers of muscle health in a 22-month-old mouse to levels
similar to a 6-month-old mouse."
About PURENERGY®:
ChromaDex's PURENERGY® is a novel, patented next-generation caffeine ingredient derived
from the technology-driven fusion of caffeine and ChromaDex's patented pTeroPure®
pterostilbene. The first human study of PURENERGY® demonstrated PURENERGY® delivers
30% more caffeine, stays in the blood stream longer, and is absorbed more slowly than ordinary
caffeine. With PURENERGY® formulators of energy products may have the ability to reduce the
total amount of caffeine in their products by as much as 50% without sacrificing consumers'
expectations from such products. Clinical studies show the ingredient provides a rapid and
sustained energy boost for at least 6 hours and the potential for a more moderated and gradual
finish, preventing the "crash" experienced with caffeine products.
In addition to being a caffeine alternative, PURENERGY® has additional health benefits
stemming from its co-crystal formulation with pTeroPure® pterostilbene, which has shown great
promise for supporting heart health, cognitive function, anti-aging, weight loss and other
metabolic functions. pTeroPure® was named the 2010 North American Most Promising
Ingredient of the Year by the independent research company Frost & Sullivan.
About 5LINX®:
5LINX® offers a unique work from home business opportunity based on essential products and
services people use every day. 5LINX® distributes its products and services through a network
of dedicated independent marketing representatives. 5LINX® has earned the distinction as being
recognized by Inc. Magazine as one of the 5,000 fastest-growing privately-held companies in the
country for the last eight years. In 2011 and 2012, 5LINX® was named to the Global 100 List
compiled by Direct Selling News, the premier direct sales industry magazine, making 5LINX®
one of the 100 largest direct sales companies in the world.
About ChromaDex®:
ChromaDex is an innovative natural products company that discovers, acquires, develops and
commercializes proprietary-based ingredient technologies through its unique business model that
utilizes its wholly owned synergistic business units, including ingredient technologies, natural
product fine chemicals (known as "phytochemicals"), chemistry and analytical testing services,
and product regulatory and safety consulting (as Spherix Consulting). The company provides
seamless science-based solutions to the nutritional supplement, food and beverage, animal
health, cosmetic and pharmaceutical industries. The ChromaDex ingredient technologies unit
includes products backed with extensive scientific research and intellectual property. Its
ingredient portfolio includes pTeroPure® pterostilbene; ProC3G®, a natural black rice containing
cyanidin-3-glucoside; PURENERGY® a caffeine-pTeroPure® co-crystal; and NIAGEN™, its

4. recently launched branded nicotinamide riboside, a novel next-generation B vitamin. To learn
more about ChromaDex, visit www.chromadex.com.
Forward-Looking Statements:
This release contains forward-looking statements within the meaning of Section 27A of the
Securities Act of 1933, as amended, and Section 21E of the Securities and Exchange Act of
1934, as amended. Statements that are not a description of historical facts constitute forward-
looking statements and may often, but not always, be identified by the use of such words as
"expects", "anticipates", "intends", "estimates", "plans", "potential", "possible", "probable",
"believes", "seeks", "may", "will", "should", "could" or the negative of such terms or other
similar expressions. Actual results may differ materially from those set forth in this release due
to the risks and uncertainties inherent in the Company's business. More detailed information
about the Company and the risk factors that may affect the realization of forward-looking
statements is set forth in the Company's Annual Report on Form 10-K for the fiscal year ended
December 28, 2013, the Company's Quarter Reports on Form 10-Q and other filings submitted
by the Company to the SEC, copies of which may be obtained from the SEC's website at
www.sec.gov. Readers are cautioned not to place undue reliance on these forward-looking
statements, which speak only as of the date hereof. All forward-looking statements are qualified
in their entirety by this cautionary statement and the Company undertakes no obligation to revise
or update this release to reflect events or circumstances after the date hereof.
ChromaDex Media Inquiries:
Beckerman PR
Jerry Schranz
201-465-8020
jschranz@beckermanpr.com
ChromaDex Company Contact:
Laura Kelly, Executive Assistant
949-419-0288
laurak@chromadex.com
5LINX Company Contact:
William Faucette, Jr.
Vice-President of North American Sales
585-576-4023
wfaucette@5linx.com
Statements in this press release have not been evaluated by the Food and Drug
Administration. Products or ingredients are not intended to diagnose, treat, cure or
prevent any disease.
SOURCE ChromaDex Corp.

5. Nicotinamide Riboside (NR/Niagen) personal
experience thread
Started by APBT , Apr 03 2014 10:42 PM
nicotinamide ribo nr niagen nad niagen sinclair hpn n(r)
492 replies to this topic
#31 BobSeitz

 Registered User
 77 posts
 107 ₮
 Location:Huntsville, AL
Posted 11 April 2014 - 02:07 AM
It's time for my Thursday update.
Once again, I've taken 15 Niagen capsules so far today, with another 5 reserved for bedtime. (I
didn't mention the fact that I take two 100-mg. capsules of the new Xymogen bioabsorbable s-
acetyl glutathione capsules every day along with a transdermal glutathione cream, and 8-hour,
sustained release n-acetyl cysteine capsule at bedtime.)
Once again, I headed for the Wellness Center this morning. But it has occurred to me that I've
probably added a few pounds over the past two months. I've been concerned about getting
enough protein to support the rebuilding of muscles, but that mindset may have invited extra
calories. (I don't weigh myself very often, since I'm not normally focused on losing weight.)
Chinning myself is a sensitive function of how heavy I am. A couple of pounds can make a
sizable difference. And since we're trying to determine whether Niagen is making meaningful
differences, my weight needs to be tightly controlled. I'll check my weight when I wake up
tomorrow morning, and try to lean toward a weight-loss mode over the next few weeks.
At the Wellness Center this morning, I tried for the first time elevating the treadmill. I found that
at a slope of 10°, I was generating 7.4 METs at 3 miles an hour, and 8 METs at 3.3 miles an
hour. I ran about ½ mile at 3 mph. (7,4 METs) and about ½ mile at 3 mph (8 METs). My pulse
rate gradually climbed from the 90's to the low 120's. I ran another mile, and then began running
60-second sprints at 9,5 METs, resting about two minutes between sprints... except that today, I

6. raised the bar a bit by running 120-second sprints. My highest pulse rate was 130, which is
below my maximum pulse rate by any standards. (I've been reticent about pushing myself harder
without anyone watching my EKG.) I was breathing hard enough to make it hard to carry on a
conversation by the time I finished.
Last night, I began investigating to find out what the norms are for someone my age. Here they
are:
Heart Rate:
I found two formulas for this. The classical formula is 220 - (your age). For me, at 85, that
works out to be 135 beats per minute.
Another formula that purports to update the above formula uses 208- 0.7(your age). This
formula yields 148.5 beats per minute.
Maximum Level of Exertion (METs Level):
For someone who's athletically conditioned, the maximum treadmill METs level is given by
18.7- 0.15 X your age. For me at 85, that works out to be 5.95 METs. Of course, I ran today for 6
minutes at 8 METs without reaching my maximum heart rate.
If I use 8 METs as my maximum level of exertion, subtract 5.95 METs from it, and divide by
0.15 to arrive at an age-equivalent METs level, I arrive at 2.05/0.15 = 13⅔ years. Subtracting this
from 85 produces an equivalent "physiological age" of 71⅓ years.
Of course, I ran two minutes at 9.5 METs without reaching my maximum heart rate. If I used 9
METs (conservatively( as my peak treadmill level of exertion, I would arrive at an offset of
3.05.0.15 = 20⅓ years. Subtracting this from 85 gives a physiological age of 64⅔ years.
Of course, this doesn't quite give the picture. Our 7½-year-old darling and I run races and play
tag and Frisbee and hide-and-go-seek (including running for home base). Our 40-year-old
daughter, Lisa, died in her sleep in December, 2006, and left her three-month-old baby, Amber,
for my wife and I to rear, so we've been back on the "Daddy and Mommy" track.
After running on the treadmill, I went to the "Chin-Lift" and tried chinning myself. I was just
barely able to pull myself up with 4 pounds of lift. At 10 pounds of lift, I handily chinned myself
12 times. quitting before I quite reached my limit.
My athletic fitness testing in January when I first approached the Wellness Center put me in the
95th percentile for VO2 max at about 30 (Mkm). I inquired about the age group to which that
percentile ranking applied, but the personal trainer who administered the January fitness exam.
didn't know. On the Partial Curl-Up (Rep) ranking, I was in the 99th percentile in January. I was
also in the 99th percentile on the waist-to-hip ratio. I was in the 89th percentile for body fat
composition. On other measures testing flexibility, etc., I didn't fare as well.
Presumably, my workouts at the Wellness Center will improve some of these benchmarks over
time.
The verdict for Niagen? It depends upon how long it takes Niagen to do its thing in humans, and
it depends upon how much weight (if any) I may have added since the last time I weighed

7. myself. There hasn't been any "Wow!" boost in my performance over the past 48 hours.
There is a lot of buzz recently in anti aging forums surrounding a new study from David
Sinclair of resveratrol fame
http://hms.harvard.edu/news/genetics/new-reversible-cause-aging-12-19-13
Niacinimide Riboside ( Niagen ) has been shown to come close to what Sinclair used to
boost NAD, more so than regular niacin. There is evidence that CD38 reduces NAD+ and
inhibiting it with flavonoids like C3G, apigenin etc is synergistic.
" We show that pharmacological inhibition of CD38 results in higher intracellular
NAD+ levels, Finally, apigenin administration to obese mice increases NAD+ levels"
http://diabetes.diabetesjournals.org/content/62/4/1084.short
I think that a supplement combining the two would be important for LEF to consider. I am
already supplementing this on my own with what is available. The problem with flavonoids
for CD38 inhibition is bioavailability ....this is where LEF's expertise could come into play
By Beefster - 3/6/2014 8:34:44 AM
The use of the flavonoids is interesting for the support of NAD. The use of Niagen is over
rated. I bought a couple of bottles of this product early on and have since discovered that it
is only twice as potent as niacinamide as far as increased NAD. The Niagen is expensive and
the capsules are only 100 mg in their max dosage cap. However, nacinamide is much more
effective than niacin in obtaining higher NAD levels.
The discovery itself is a further update and some clarification of old news for the use of
niacinamide with resveratrol. Go to Life Enhancements site type in Krebs cycle or
niacinamide and see that they have been recommending the use of niacinamide since the
early MIT studies years ago.
By fastingly - 3/7/2014 2:12:34 PM
"However, nacinamide is much more effective than niacin"
Somebody correct me if I'm wrong here, but isn't there some independent benefit of the
"niacin flush" you can only get from niacin?
I long ago read some health guru make the pitch about how the niacin flush will clean your
arteries and otherwise does all sorts of amazing things for your cardiovascular system that
cannot come from niacinamide.
I make a point to subjecting myself to an intense, red, all body flush with a treatment of
about 750 mg of quick-release niacin taken once per month (in addition to daily, regular
supplementation).
Anyone care to advise me further/otherwise?

8. Thanks!
By xnlife - 3/7/2014 6:46:15 PM
Sounds good but its a three things you should be taking maybe four with niacin
Q10 blend with Omega-33333
Resveratrol
DHEA
Good multi -calcium good amount of Vit D
By Kevnzworld - 3/8/2014 8:18:18 AM
Nicotinamide riboside is different from Niacinimide or niacin, and is a more direct NAD+
activator. It's mechanism of action has nothing to do with the flushing effect that niacin
users want to increase HDL.
It's the closest one can get to the molecule used in the Sinclair study.
" In eukaryotes, nicotinamide riboside is a newly discovered NAD+ precursor that is
converted to nicotinamide mononucleotide by specific nicotinamide riboside kinases, Nrk1
and Nrk2. In this study, we discovered that exogenous nicotinamide riboside promotes Sir2-
dependent repression of recombination, improves gene silencing, and extends lifespan
without calorie restriction. "
http://www.cell.com/retrieve/pii/S009286740700390X?cc=y
By Beefster - 3/9/2014 12:27:11 PM
Just so readers don't get confused nicotinamide and niacinamide are two different terms
used interchangeably to describe the same thing. Nicotinamde riboside is a form of B3 found
in milk and beer that is effective in increasing NAD. Niagen is the commercially available
product for nicotinamide riboside.
For those interested the link below is another good article on NAD:
http://www.life-enhancement.com/magazine/article/2947-its-a-nad-nad-nad-nad-world
Some older articles for further reading on NAD:
http://www.life-enhancement.com/magazine/article/142-new-gene-supplements-may-
extend-your-life
http://www.life-enhancement.com/magazine/article/484-nicotinamide-golden-thread-in-
the-tapestry-of-life
http://www.life-enhancement.com/magazine/article/606-nicotinamide-links-calories-genes-
and-antiaging
By Kevnzworld - 3/29/2014 8:24:20 AM
albedo (3/29/2014)

9. OK, this is from a commercial site but could be an interesting recap on why NAD+
activation:
http://www.smart-publications.com/articles/resveratrol-niacin-nicotinamide-riboside-key-
players-in-activating-sirtuins-to-mimic-calorie-restriction-extend-lifespan-part-i
I also would like LEF offering something in this space.
Interesting read. The author is familiar , I think he also writes for LEF. The Leonard
Guarante lecture should be interesting. David Sinclair did his doctoral work under his
supervision I believe.
Let us know if anything new is revealed ....
By Kevnzworld - 3/29/2014 3:13:21 PM
DDye (3/29/2014)
Do you know who the author is?
John Morgenthaler I think. He did the flow charts.
By fastingly - 4/6/2014 12:23:18 PM
In the buzz online about Niacinimide Riboside, a lot of mentions of it occurring naturally in
beer and milk. Obviously, nobody is going to experience any of the experimental-level type
of mouse-rejuvenating effects from doses naturally found in food.
But just as a curiosity, does anybody have data on exactly how much is contained in these
foods?
Beer appears to contain about 1.8 mg of niacin per serving, as does a couple of tablespoons
of brewer's yeast. Now, is the naturally-occurring niacin therein all or mostly in this unique
form? Brewers yeast is one of those healthy health foods long touted since the beginnings
of the "good food" movement of the late 1920's., I'm wondering about the niacin in it as
one of its many good-for-you factors that people since long ago have made testimonials
about?
Personally, I find brewer's yeast a bit repulsive. Last time I had some on hand, I ended-up
feeding my compost pile the bulk of it and had decided that would be the last time I would
ever try that nasty stuff again, but now I'm giving it another look.
By alc - 4/15/2014 6:10:48 PM

10. After I red about the study that prof. Sinclair did and published in December, I googled for
NAD+
I found out that Biotivia is developing a product that they claim is doing the same thing, like
the study did.
http://www.biotivia.com/
You might read on their web site about MitoTrans* (NAD+)
http://www.biotivia.com/nad_plus_mitotrans_antiaging.html
I'm interested in reading others comments/thoughts about this product that Biotivia is
producing.
Adrian
By Kevnzworld - 4/16/2014 11:00:36 AM
alc (4/15/2014)
After I red about the study that prof. Sinclair did and published in December, I googled for
NAD+
I found out that Biotivia is developing a product that they claim is doing the same thing, like
the study did.
http://www.biotivia.com/
You might read on their web site about MitoTrans* (NAD+)
http://www.biotivia.com/nad_plus_mitotrans_antiaging.html
I'm interested in reading others comments/thoughts about this product that Biotivia is
producing.
Adrian
Interesting. I have read somewhere that NAD itself is too large of a molecule to be
absorbed. Perhaps their delivery system gets around that. There isn't any reliable data
regarding NAD enhancement in humans with any specific product or dosage of
products. According to Guerante and Sinclair, NR being a small molecule is the best and
most direct precursor of NAD
This is another reason that it would be interesting to see how LEF addresses this, and which
molecule they will choose to use. The problem with anything other than plain old niacin, is
patent issues.
By DrWells - 5/18/2014 9:25:50 PM
Niagen is NR cholride and not NR. I suspec the chloride form has bioactive differences than

11. NR. NR is very expensive. NR chloride is much less expensive.
By fastingly - 7/9/2014 5:40:21 AM
In light of this information on the importance of NAD, would anyone advise if one should
continue taking an NADH supplement? It sounds like this pseudohypoxia cell-aging occurs
with imbalanced NADH at the mitochondrial cell level, so is taking a dietary supplement of
NADH therefore somewhat counterproductive to the goals of life extension?
By AstroPinion - 10/13/2014 6:05:12 PM
Hi,
Very interesting stuff. It would be reassuring to see info regarding safety and known side
effects.
By Paul1917 - 10/21/2014 4:57:42 PM
I was just reading about it after seeing the new supplement and wondered if this reference
has any relevance to NR?
http://www.ncbi.nlm....les/PMC3318212/
"Depletion of available methyl groups, whether from a poor diet or one with excessive
nicotinamide, may be a common cause of epigenetic phenomena given the marked influence
methylation has on gene expression in pluripotent and differentiated cells that maintain cellular
identity [265]. The methylome is an important mediator of information and modifies gene
transcription and translation including riboswitches affecting RNA metabolism and in toxicology
pathways in concert with NAD and ATP and all appears to be involved in a number of diseases
seen within the pellagra phenotype such as cancer and motor neurone disease [267–272]. A
major role for the methylome is well accepted for many cancers and their related epimutations
sometimes working in concert with DNA sequence mutations in tumour suppressor and DNA
repair genes and may well be modifiable by diet [273–277]. There may be several mechanisms
whereby such metabolic programming can be inherited and affect offspring [278]."
By Paul1917 - 10/23/2014 3:23:54 PM
Paul1917 (10/21/2014)
I was just reading about it after seeing the new supplement and wondered if this reference
has any relevance to NR?
http://www.ncbi.nlm....les/PMC3318212/
"Depletion of available methyl groups, whether from a poor diet or one with excessive

12. nicotinamide, may be a common cause of epigenetic phenomena given the marked influence
methylation has on gene expression in pluripotent and differentiated cells that maintain cellular
identity [265]. The methylome is an important mediator of information and modifies gene
transcription and translation including riboswitches affecting RNA metabolism and in toxicology
pathways in concert with NAD and ATP and all appears to be involved in a number of diseases
seen within the pellagra phenotype such as cancer and motor neurone disease [267–272]. A
major role for the methylome is well accepted for many cancers and their related epimutations
sometimes working in concert with DNA sequence mutations in tumour suppressor and DNA
repair genes and may well be modifiable by diet [273–277]. There may be several mechanisms
whereby such metabolic programming can be inherited and affect offspring [278]."
sorry link was dead.
the proper link is below
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3318212/#!po=27.3333
interesting is the fact that NAD ratio's cannot yet bemeasured but if one reads the beginning of
the document there is mention thatas one ages there is possible degradation in the pathway so it
might be aproblem for many and supplementation may have some benefit or unknown affect.
now on page 21 cited above there is "This balance NAD+:NADH maybe moreabout...."
then the next page is reference to under/over supply with more references.
My thoughts are if one has some under/over methylation that is not corrected byfolate or B-12 or
similar used to correct say MTFR defects then one might tipthe balance.
http://www.ncbi.nlm.nih.gov/pubmed/19812215
What is intriguing for me is this reference
http://www.ncbi.nlm.nih.gov/pubmed/19076449
By Paul1917 - 11/5/2014 4:22:56 PM
I'm in the process of trying this test
http://www.adma-test.co.uk/
and Dr Ben Lynch I think still gives his interpretation of the results of this test that might
help resolve high homocysteine
http://www.seekinghealth.com/media/Methylation%20_Profile_%20Sample_%20Report.pdf
By Paul1917 - 11/9/2014 7:36:35 AM

13. "Probiotic Yeasts Stimulate NAD+ – Increase Metabolism, Longevity
http://www.realnatural.org/probiotic-yeasts-stimulate-nad-increase-metabolism-
longevity/?utm_source=feedburner&utm_medium=feed&utm_campaign=Feed%3A+real-
natural+%28Real+Natural%3A+Responsible+Natural+Health+News%29
I see that the first reference is Oct 2014 so this is quite recent and I habe his probiotics
book and was exploring his profile and found this article.
By Kevnzworld - 12/4/2014 5:11:39 AM
DDye (12/3/2014)
The article above isn't opening for me.
The daily dosage in Life Extension's product is based on preliminary human data and a
pending human dosage study.
I hope LE follows up with more data regarding the bioavailability and pharmakinetics of NR
and any studies that show NR taken orally increases NAD+ in humans, and if so, at what
dose. Anecdotally most people on various blog sites feel that somewhere between 500-
1000 mg's is where they feel something. I take 625 mg, but am awaiting actual real
data......
Currently, there isn't any AFAIK
By Kevnzworld - 12/5/2014 4:21:48 PM
albedo (12/4/2014)
Hi Kevnzword.
Yes, this is what I was referring to. Probably, as with many supplements, you can "feel"
something if you are in a status of nutrient deficiency and/or have a pathology while, when
this is not the case, you put trust in the studies which show benefit and you balance with
risks. That is also why it is important to find (healthy) human studies. For NR do I
understand correctly ChromaDex is also coming up with a optimal human dose? Of course I
would like to read LEF on this. It reminds me a bit the discussions on trans-resveratrol
dosage when people also mega-dosed while you also had studies showing benefits at
relatively low doses.
The key is to establish whether or not taking NR orally actually raises NAD, and how much is
necessary. The studies that Chromadex is currently conducting are looking at safety and
pharmakinetics, which are different.
It seems like that's all pointless if orally administered NR doesn't raise NAD significantly.
I guess we hope, and wait.....
By telomere - 1/13/2015 6:20:29 PM

14. I'm also really interested in the optimal dosage of NR, and best means of restoring NAD+
levels. I'm hoping that the Chromadex studies will reveal this soon, because it certainly
would be a lot of wasted money if NR isn't orally bioavailable.
Anyway, i've been posting around a bit because I recently finished a month-long trial of
Biotivia's MitoTrans, which is their stabilized NAD+ supplement. Ive tried both niagen and
MitoTrans, in attempts of improving my chronic fatigue, lack of motivation, and low physical
endurance.
I can't say I really noticed all that much from niagen - very slight improvement in energy
maybe, and no change in body composition or really any other noticeable effects.
With MitoTrans, while the effects are subtle, i seem to have lost a pretty significant amount
of body fat and have a noticeably improved capacity for endurance exercise (like walking for
long distances or jogging). I admit that these could possibly be placebo, but I am very
impressed by the reduction in body-fat that ive noticed. i wish i had taken some before and
after pictures, but i'm not sure how useful that would have been since i've also started a
consistence weightlifting routine.
I would be very interested in knowing how MitoTrans compares to Niagen in terms of
bioavailability and raising of NAD+ levels. From what i understand, when NR is ingested as
precursor of NAD+, it is always NMNAT enzyme dependent pathway. and direct
supplementation of NAD+ is not enzyme dependent. so I would assume that MitoTrans is a
good bit more effective than niagen. At least, this was my rationale for trying it.
By alc - 1/14/2015 8:02:56 AM
I started to take Niagen this morning. I'm curious if I'll see any results as I'm 45. Some
people younger than me said that they had "visible" results with Niagen. I hope that is the
case as I do not like this to be another overhyped supplement.
Besides Biotivia (which is quite expensive $475 for one month!), there is RevGenetics that
will have a new product on the market.
I wonder if LEF is conducting any medical research in this area of NAD+?
The way I see it, is that once some rejuvenation pathway is identified, it is worth exploring
it and improve upon initial discoveries, either by delivery mechanism, or new molecules,
etc. And LEF do have the resources to take on such tasks. Similar LEF can look into GDF-
11, MitoQ , SkQ1 or TA-65.
I wonder why LEF is not conducting any research with these factors/molecules?
By Birage - 1/15/2015 2:29:44 AM
I started on Life Extension's NAD formula after it came out. I really did not expect to feel
anything different, especially since the dose seemed to be half of what you see from other
purveyors. I basically added it for the anti-aging science, but noticed something
positive. Normally, at the end of the day after work I tend to feel inclined to either take a
short nap, or lay on the sofa and watch TV for bit just to relax and decompress after the

15. stress of the day. I noticed almost a day or two after adding the NAD formula to my
regimen that I would continue on without the desire to lay down or take a nap. There was
nothing new other than that formula that changed in my diet, vitamins, routine, etc., so I
new it had to be the NAD formula. I have been on it for weeks and I still have that constant
energy state, not a revved up state, but just a smooth lasting energy. Has any one else
experienced this using the Niagen?
By MrGeorge - 1/15/2015 4:29:44 AM
Birage (1/15/2015)
I started on Life Extension's NAD formula after it came out. I really did not expect to feel
anything different, especially since the dose seemed to be half of what you see from other
purveyors. I basically added it for the anti-aging science, but noticed something
positive. Normally, at the end of the day after work I tend to feel inclined to either take a
short nap, or lay on the sofa and watch TV for bit just to relax and decompress after the
stress of the day. I noticed almost a day or two after adding the NAD formula to my
regimen that I would continue on without the desire to lay down or take a nap. There was
nothing new other than that formula that changed in my diet, vitamins, routine, etc., so I
new it had to be the NAD formula. I have been on it for weeks and I still have that constant
energy state, not a revved up state, but just a smooth lasting energy. Has any one else
experienced this using the Niagen?
I am trying some from live cell research, it's 250 mg of Niagen. Right now I'm into my
second day.
The first day I felt a little tired, more than usual. The second day, the same.
I will keep monitoring the results, two days isn't enough and could be coincidental.
By MrGeorge - 1/28/2015 7:54:21 AM
i've been looking over the internet for users reviews of Niagen. At first there were some that
felt results.
Some of the users went up to a 1000 mg dose.
Now after awhile there is hardly any one reporting any kind of noticeable benefit, including
this forum.
I am taking 250 mg first thing in the morning, and now have bumped it up to 500 mg.
Still not noticing benefit but maybe it is doing some good, don't know.

16. Has anyone received noticeable benefit as yet?
By Green Lantern - 2/10/2015 7:57:18 AM
Hello,
I am wondering: I am already taking the LEF Mix with added niacin. Would it be worth it to
add nicotinamide riboside to what I am already taking? Would the benefit be worth it?
Thanks,
GL
By DDye - 3/6/2014 7:49:31 AM
hi Kevn:
Your suggestion has been forwarded to Life Extension's Product Development Department.
D Dye
Moderator
By DDye - 3/7/2014 4:18:02 PM
Fastingly,
The niacin flush is part of the circulatory system benefit of niacin, which can favorably
impact lipids, despite a recent study that concluded that niacin had no protective effect (and
it may not have one in some people). The histamine-releasing effect of niacin has other
benefits. Niacinamide and niacin also may have longevity benefits, yet niacinamide's effect
in this area has been argued both ways.
D Dye
By DDye - 3/29/2014 1:11:20 PM
Do you know who the author is?
By DDye - 3/29/2014 6:06:04 PM
He was not one of Life Extension's writers.

17. By DDye - 4/10/2014 11:26:58 AM
There may still be some on the market labeled "molasses grown" that are milder tasting
than some.
By DDye - 4/14/2014 7:13:46 AM
Albedo,
Product Development provided the following response:
We greatly appreciate the feedback and are continuously scouring emerging
research for synergistic and novel ingredients. However, we do not disclose
information regarding our plans for future product development.
By DDye - 12/3/2014 1:44:56 PM
The article above isn't opening for me.
The daily dosage in Life Extension's product is based on preliminary human data and a
pending human dosage study.
By DDye - 12/4/2014 7:42:21 AM
enNicotinamide Riboside
N (R) Niagen Nicotinamide Riboside
$47.99

18. (as of 08/18/2014 at 22:16)
SKU: B00L5Q951S. Category:VitaminB3.
 Description
 Reviews
Product Description
More About Niagen: NR is a naturally-occuring vitamin B3 metabolite; Has been shown to boost
NAD+ levels higher than Niacin(2); Does not cause undesirable flushing associated with
Niacin(1); Promotes mitochondrial health* - NR maintains and enhances mitochondrial health by
boosting NAD+ levels and inducing creation of new mitochondria(2); Supports beneficial effects
on blood lipids by maintaining healthy cholesterol levels already within mormal range(2)*;
Increases sirtuin activation, improving mitochondrial activity to prevent cellular damage due to
free radicals(3)*. What is NAD+? Nicotinamide adenine dinucleotide (NAD+) is an essential
metabolite in all human cells. More About NAD+: Vital for mitochondrial health; Plays a key
role in cellular metabolism and energy production; NAD+ levels decrease with age; Rate-
limiting co-substrate for sirtuins. *These statements have not been evaluated by the FDA. This
product is not intended to diagnose, treat, cure or prevent any disease.
Features
 Breakthroughnatural supplementforbrainhealth,metabolism,andanti-aging
 Increasesthe NAD+coenzyme forenhancedmitochondrialfunction
 Promotesanti-agingeffectsfromfree radicals
 Promoteshealthycellularmetabolism*
 Niagenisthe firstandonlycommerciallyavailableformof nicotinamide riboside(NR),ahighly
effectiveNAD+boosterand"noflush"formof vitaminB3 (niacin) thatoffersmanyhealth
benefits,includingendurance,performance,weightmanagement,cardiovascularhealth,anti-
aging,cognitive functionandneuroprotection.
Related Products

Timed Release Niacin 500 mg 100 Tabs by Rugby

19. $5.75
(as of 08/18/2014 at 22:22)

Niacin 50 mg 50 mg 100 Tabs by Nature’s Blend
$2.46
(as of 08/18/2014 at 22:21)

21st Century Niacinamide 500 mg Prolonged Release Tablets, 110-Count
$4.99
(as of 08/18/2014 at 22:18)

20. 
Solgar – No-Flush Niacin, 500 mg, 250 veggie caps
$39.18
(as of 08/18/2014 at 22:20)
This really is High Performance Nutrition What thinking of Here we now have information
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Review N (R) Niagen Nicotinamide Riboside – 60 capsules
More About Niagen: NR is a naturally-occuring vitamin B3 metabolite; Has been shown to boost
NAD+ levels higher than Niacin(2); Does not cause undesirable flushing associated with
Niacin(1); Promotes mitochondrial health* – NR maintains and enhances mitochondrial health
by boosting NAD+ levels and inducing creation of new mitochondria(2); Supports beneficial
effects on blood lipids by maintaining healthy cholesterol levels already within normal
range(2)*; Increases sirtuin activation, improving mitochondrial activity to prevent cellular
damage due to free radicals(3)*. What is NAD+? Nicotinamide adenine dinucleotide (NAD+) is
an essential metabolite in all human cells. More About NAD+: Vital for mitochondrial health;
Plays a key role in cellular metabolism and energy production; NAD+ levels decrease with age;
Rate-limiting co-substrate for sirtuins. *These statements have not been evaluated by the FDA.
This product is not intended to diagnose, treat, cure or prevent any disease.
These information might help you choose in choosing products. See all Customers reviews
High Performance Nutrition Feature

21.  Breakthroughnatural supplementforbrainhealth,metabolism,andanti-aging
 Increasesthe NAD+coenzyme forenhancedmitochondrialfunction
 Promotesanti-agingeffectsfromfree radicals
 Promoteshealthycellularmetabolism*
 Niagenisthe firstandonlycommerciallyavailableformof nicotinamide riboside(NR),ahighly
effectiveNAD+boosterand“noflush”formof vitaminB3(niacin) thatoffersmanyhealth
benefits,includingendurance,performance,weightmanagement,cardiovascularhealth,anti-
aging,cognitive functionandneuroprotection.
Product selection should look at the many optional features to ensure quality and style shapes
that suit our needs this N (R) Niagen Nicotinamide Riboside – 60 capsules may be right for you.
However, you should make a decision on Read the details fully.
This can be N (R) Niagen Nicotinamide Riboside – 60 capsules What you are looking for Here
we have information Product you want And what you are interested in, we put them collectively.
You can read above To be able to study the details in regards to the product. Also you can Be
attributed to the decision about the product. Through the time many of you might not know about
the products they’re seeking. And the information is not sufficient to produce a decision. That
might produce a quality product. But here we’ve got compiled important information. Many of
these studies have You have to study them a functioning model. Composition of the item,
including accessories that have. Products you are considering. Including warranty.
Post navigation
High Performance Nutrition Feature
 Breakthrough natural supplement for brain health, metabolism, and anti-aging
 Increases the NAD+ coenzyme for enhanced mitochondrial function
 Promotes anti-aging effects from free radicals
 Promotes healthy cellular metabolism*
 Niagen is the first and only commercially available form of nicotinamide riboside (NR), a
highly effective NAD+ booster and “no flush” form of vitamin B3 (niacin) that offers
many health benefits, including endurance, performance, weight management,
cardiovascular health, anti-aging, cognitive function and neuroprotection.
Product selection should look at the many optional features to ensure quality and style shapes
that suit our needs this N (R) Niagen Nicotinamide Riboside – 60 capsules may be right for you.
However, you should make a decision on Read the details fully. Read More Reviews!!!

22. This can be N (R) Niagen Nicotinamide Riboside – 60 capsules What you are looking for Here
we have information Product you want And what you are interested in, we put them
collectively.You can read above To be able to study the details in regards to the product. Also
you can Be attributed to the decision about the product.Through the time many of you might not
know about the products they’re seeking. And the information is not sufficient to produce a
decision. That might produce a quality product. But here we’ve got compiled important
information. Many of these studies have You have to study them a functioning model.
Composition of the item, including accessories that have. Products you are considering.
Including warranty.