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Tosoh scientists developed novel non-porous cationic exchange resins, marketed as TSK-GEL SP-STAT and TSK-GEL CM-STAT columns. The cation exchange columns are packed with 7 and 10μm spherical, mono-disperse, non-porous particles of which the surface has been modified with open access multi-layered cation exchange groups. The cation exchangers show higher adsorption capacities and require lower pressures compared with conventional non-porous columns of the same column dimension. Rapid separations of proteins were achieved within 1 minute on short columns (3.5cm) packed with 10μm resin and high resolution analyses were achieved on a 10cm length column packed with 7μm resin. The basic properties of the novel cation exchange columns and how they apply to the separation of proteins, antibodies and peptides are reported in comparison with commercially available non-porous CIEX columns.

TSK-GEL STAT Cation Exchange Columns (TP132)

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TSK-GEL STAT Cation Exchange Columns (TP132)

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