Computers are particularly well suited to searching through the billions of bases in any genome looking for important information that can alter treatments and save lives. However, if done incorrectly, computers can take forever to find even the simplest portions of sequence in the genome. In this discussion we will be showing practical information and examples about how to correctly encode genomic sequences for super fast, programmatic search. We’ll explore in detail how computers work, think, and read. Given the right “genomic language”, they can be our greatest, and fastest, allies in finding malicious variants. Timothy Collinson

1. Reading the Human Genome
is Hard Work
Tim Collinson

2. Who is Tim?
2
• A long time programmer
• Worked for some interesting companies!
• Technical Fellow working at HP Labs
• Interested in technical challenges
• Has a pretty big family (wife and 6 kids)
• Scout Master (plenty of experience with
Blood)

3. Why is reading the human genome hard?
3
DNA seems quite simple:
A C G T
(Note: RNA is nearly as simple)

4. Why is reading the human genome hard?
4
The human genome has a few
of those simple letters
smashed together in pairs…

5. Why is reading the human genome hard?
5
3,200,000,000*
* or so, give or take

6. Why is reading the human genome hard?
6
Add image showing entire
genome here*
* We would need 40,000 slides, or so, to show it**
** This would make my presentation boring***
*** This is a wild assumption, bare with me

7. Reading the human genome is hard?
7
Finding patterns in letters can be simple!
Let’s try:

8. Reading the human genome is hard?
8
Find the changed base:
Normal - A C G T T G C A
Variant - A C G T G T C A

9. Reading the human genome is hard?
9
Let’s try an example in the BRCA1 Gene*
* It’s slightly larger

10. Reading the human genome is hard?
10
ATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAA
GTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGAT
GAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCAC
CAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGT
TTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGT
GGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAG
CATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGC
AGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTA
CGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACA
GTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAG
TGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAG
TCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTG
CAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGG
TACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACG
ATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGT
ACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTG
CACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACT
TGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTC
AAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGAT
CAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAA
AGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGA
TGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCA
CCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTG
TTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAA
GTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCA
GTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTG
CAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGT
ACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGAC
AGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAA
GTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATC
AGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGT
GCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACG
GTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTAC
GATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAG
TACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGT
GCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAA
CTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTT
CAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGA
TCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGA
AAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTG
ATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTC
ACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACT
TGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCA
AGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATC
AGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGT
GCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACG
TACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGA
CAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACA
AAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGAT
CAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATG
TGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTAC
GGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTA
CGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACA
GTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAA
GTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCA
ACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTT
TCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGG
ATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCG
AAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCT
GATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAG
TCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAAC
TTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTC
AAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGAT
CAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATG
TGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTAC
GTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGAT
GACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTAC
AAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGA
TCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCAT
GTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTA
CGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGT
ACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGA
CAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAA
AGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACC
AACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTT
TTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTG
GATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATC
GAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTC
CTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAA
GTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAA
CTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTT
CAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGA
TCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCAT
GTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGT
ACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGA
TGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTA
CAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGG
ATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCA
TGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAG
TCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTAC
GTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATG
ACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACA
AAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCAC
CAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGT
TTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAG
TGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAA
TCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGT
CCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGA
AGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCA
ACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTT
TCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTG
GATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTC
ATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAG
TACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACG
ATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGT
ACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTG
GATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGT
CATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCA
GTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTCGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTA
CGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACGGTACGTACGATGACAGTACAAAGTGCACCAACTTGTTTTCAAGTGGATCAATCGAAAGTCCTGATGAAGTCACCAACTTGTTTTCAAGTGGATCAGTCATGTGCAGTACGTACGATGACAGTACAAAGTGGATCAGTCATGTGCAGTACG
BRCA1 has about 81,000 bases.
Please find the variant base.
I’ll give you a hint, it’s located at 65,237.
Go ahead, I’ll wait.

11. Reading the human genome is hard?
11
Easy right?
Computers can’t read text much better than
humans.

12. Wait, what? Aren’t computers fast?

13. Computers are fast?
13
Computers can be fast, but computers are
stupid.
They must be taught to do everything.
Deep down, they don’t do much.

14. Computers are fast?
14
Sometimes we try to teach computers to think
like humans.
The cognitive dissonance between humans and
computers is reduced this way.

15. Computers are fast?
15
Human’s would rather look at this:
A C G T
Than this:
00011011
But let’s not get ahead of ourselves.

16. Chemistry and algorithms to the rescue!

17. So chemistry?
17
We’ve found chemical techniques to pinpoint
smaller sections of DNA so that we can process
less.

18. So chemistry?
18
These chemicals have downsides:
They can be very expensive
They can be very time consuming

19. So algorithms?
19
In order to reduce the amount of time needed
for chemical reactions we constantly work on
new algorithmic ways to process DNA.

20. So algorithms?
20
Many of these algorithms are used to find
patterns in text as quickly as possible.

21. So chemistry and algorithms?
21
Chemistry and algorithms help.
The cost of finding variants is being constantly
reduced.
Can we do better?

22. So how do computers think?

23. Computers think differently.
23
Computers really only manipulate bits of data:
0 1

24. Computers think differently.
24
Computer processors are highly tuned to
manipulate these bits very quickly.

25. Computers think differently.
25
We train computers to manipulate these bits in
ways we can understand.
Again, a cognitive dissonance issue.

26. Computers think differently.
26
Each time a computer processor manipulates a
bit, we call this an instruction.

27. Computers think differently.
27
Computers can process a lot of instructions.
This Mac can handle about 3,200,000,000 per
second*
Everything a computer does is a set of bitwise
instructions.
* That number seems familiar!

28. Computers think differently.
28
int i = 7;
int j = 8;
int k = i + j;
We see:
• Create a variable, called i, set to 7.
• Create a variable, called j, set to 8.
• Create a variable, called k, set to the sum of i and j.

29. Computers think differently.
29
A computer sees:
• Create a storage location of a certain size
• Name it i
• Store a value in that location
• Keep track of that location for further use
• Create a storage location of a certain size
• Name it j
• Store a value in that location
• Keep track of that location for further use
• Create a storage location of a certain size
• Name is k
• Check to see if there is a storage location named i
• Get the value
• Check to see if there is a storage location named j
• Get the value
• Use operations to sum the values from the locations found in previous instructions
• Place resulting value in to location from previous instruction
• Keep track of that location for further use

30. Computers think differently.
30
There’s an easier way to write those instructions, of course:
.cfi_startproc
0000 55 pushq %rbp
.cfi_def_cfa_offset 16
.cfi_offset 6, -16
0001 4889E5 movq %rsp, %rbp
.cfi_def_cfa_register 6
0004 C745F407 movl $7, -12(%rbp)
000000
000b C745F808 movl $8, -8(%rbp)
000000
0012 8B45F8 movl -8(%rbp), %eax
0015 8B55F4 movl -12(%rbp), %edx
0018 01D0 addl %edx, %eax
001a 8945FC movl %eax, -4(%rbp)
001d B8000000 movl $0, %eax
00
0022 5D popq%rbp
.cfi_def_cfa 7, 8
0023 C3 ret
.cfi_endproc

31. Computers think differently.
31
Who would want this:
int i = 7;
int j = 8;
int k = i + j;

32. Computers think differently.
32
When you can have this!:
.cfi_startproc
0000 55 pushq %rbp
.cfi_def_cfa_offset 16
.cfi_offset 6, -16
0001 4889E5 movq %rsp, %rbp
.cfi_def_cfa_register 6
0004 C745F407 movl $7, -12(%rbp)
000000
000b C745F808 movl $8, -8(%rbp)
000000
0012 8B45F8 movl -8(%rbp), %eax
0015 8B55F4 movl -12(%rbp), %edx
0018 01D0 addl %edx, %eax
001a 8945FC movl %eax, -4(%rbp)
001d B8000000 movl $0, %eax
00
0022 5D popq%rbp

33. Computers think differently.
33
The problem is, searching through genomic data
takes a whole lot of instructions!

34. Computers think differently.
34
As we move forward it is going to take more
than new chemistry techniques and algorithms
to find variants.
We’re also going to have to reduce the number
of instructions a computer must complete in
order to find variants.

35. So, wait, what?

36. It’s translation!
36
We’re going to need to turn these: A C G T
Into these: 0 1

37. It’s translation!
37
So:
A = 0, C = 1, G = um?, T = ut oh :(

38. It’s translation!
38
Actually:
A = 00
C = 01
G = 10
T = 11

39. It’s translation!
39
How does this help us?
Remember finding the variant?
Normal - A C G T T G C A
Variant - A C G T G T C A

40. It’s translation!
40
For a computer, if done poorly, finding the
variant in that text set of 8 bases might take
hundreds of looped instructions!

41. It’s translation!
41
Let’s look at it as a computer would:
Normal - 0001101111100100
Variant - 0001101110110100

42. It’s translation!
42
If programmed correctly, finding this variant can
be done in fewer than a dozen instructions!

43. It’s translation!
43
Come on, that was a bit contrived!
What about the BRCA1 Gene!*
* It’s slightly larger, remember.