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Home > 成員簡介 > 師資個人簡介 > 蕭明熙(Ming­Shi Shiao)
蕭明熙(Ming­Shi Shiao)
Ming­Shi Shiao
Position Professor & Chairperson
Education Ph.D. in Bioorganic Chemistry, Brown
E­mail msshiao@mail.cgu.edu.tw
Office Tel +886­3­211­8800 ext.3495
Fax +886­3­211­8700
Laboratory Laboratory of Chemical Biology
Specialty Chemical biology、lipid metabolism、
metabolomics
Lab & Research Interest
1. Diabetes­induced metabolic disorders and the effects of resveratrol for
improving lipid, energy, and BCAA metabolism and reducing of
inflammation and muscle wasting. Chen KH, Cheng ML, Jing YH, Chiu DT, Shiao
MS*, Chen JK*. Resveratrol ameliorates metabolic disorders and muscle wasting in
streptozotocin­induced diabetic rats. Am J Physiol Endocrinol Metab 301: E853–E863,
2011. First published July 26, 2011; doi:10.1152/ajpendo.00048.2011.
Diabetes mellitus (DM) is characterized by dysregulated energy metabolism.
Resveratrol (RSV) has been shown to ameliorate hyperglycemia and hyperlipidemia in
diabetic animals. However, its overall in vivo effects on energy metabolism and the
underlying mechanism require further investigation. In the present study, electrospray
ionization­tandem mass spectrometry was employed to characterize the urine and
plasma metabolomes of control, streptozotocin­induced DM and RSV­treated DM rats.
Using principal component analysis (PCA) and heat map analysis, we discovered
significant differences among control and experimental groups. RSV treatment
significantly reduced the metabolic abnormalities in DM rats. Compared with the age­
matched control rats, the level of carnitine was lower, and the levels of acetylcarnitine
and butyrylcarnitine were higher in the urine and plasma of DM rats. RSV treatment
ameliorated the deranged carnitine metabolism in DM rats. In addition, RSV treatment
attenuated the diabetic ketoacidosis and muscle protein degradation, as evidenced from
the attenuation of elevated urinary methyl­histidine and plasma branched­chain amino
acids levels in DM rats. The beneficial effects of RSV in DM rats were correlated with
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2. acids levels in DM rats. The beneficial effects of RSV in DM rats were correlated with
activation of hepatic AMP­activated protein kinase and SIRT1 expression, increase of
hepatic and muscular mitochondrial biogenesis and inhibition of muscle NF­kB
activities. We concluded that RSV possesses multiple beneficial metabolic effects in
insulin­deficient DM rats, particularly in improving energy metabolism and reducing
protein wasting.
2. Metabolomics as a tool for biomarker discovery in metabolic syndrome,
diabetes, and diabetes­accelerated atherosclerosis. Huang CF, Cheng ML, Fan
CM, Hong CY, Shiao MS*. Nicotinuric acid: A potential marker of metabolic syndrome
through a metabolomics­based approach. Diabetes Care (2013, in press)
Metabolic syndrome is a multifaceted disorder and considered a universal
epidemic puts patients on the road to type 2 diabetes and atherosclerotic cardiovascular
diseases. Although urine metabolomics has potential utility in metabolic profiling
because urine metabolites analysis reflects global outflux of metabolic change, a
surrogate biomarker in urine in fully reflecting features of metabolic syndrome has not
been explored. Therefore, the aim of this study was to elicit a potential biomarker to
picture metabolic syndrome by collecting data on subjects with overweight,
dyslipidemia, hypertension or impaired glucose tolerance and taking a metabolomics
approach to analyze the metabolites of urine revealed in metabolic syndrome.
3. Collaborative studies on the role of microRNA­122 in hepatic lipid
homeostasis, fatty liver and carcinogenesis. MicroRNA­122 plays a critical role
in liver homeostasis and hepatocarcinogenesis. Tsai WC. Hsu SD, Hsu CS,1 Lai TC, Chen
SJ, Shen R, Huang Y, Chen HC, Lee CH, Tsai TF, Hsu MT, Wu JC, Huang HD, Shiao
MS, Hsiao M, Tsou AP. J Clin Invest. 2012;122:2884­2897. (NMR spectroscopy was
used as a key tool in the metabolomics part of this collaborative study. Prof. AP Tsou,
YMU is the principal investigator.)
MicroRNA­122 (miR­122), which accounts for 70% of the liver’s total miRNAs,
plays a pivotal role in the liver. However, its intrinsic physiological roles remain
largely undetermined. We demonstrated that mice lacking the gene encoding miR­122a
(Mir122a) are viable but develop temporally controlled steatohepatitis, fibrosis, and
hepatocellular carcinoma (HCC). These mice exhibited a striking disparity in HCC
incidence based on sex, with a male­to­female ratio of 3.9:1, which recapitulates the
disease incidence in humans. Impaired expression of microsomal triglyceride transfer
protein (MTTP) contributed to steatosis, which was reversed by in vivo restoration of
Mttp expression. We found that hepatic fibrosis onset can be partially attributed to the
action of a miR­122a target, the Klf6 transcript. In addition, Mir122a–/– livers
exhibited disruptions in a range of pathways, many of which closely resemble the
disruptions found in human HCC. Importantly, the reexpression of miR­122a reduced
disease manifestation and tumor incidence in Mir122a–/– mice. This study
demonstrates that mice with a targeted deletion of the Mir122a gene possess several
key phenotypes of human liver diseases, which provides a rationale for the
development of a unique therapy for the treatment of chronic liver disease and HCC.
Publications
Publications:
1. Shiao MS, Chiu JJ, Chang BW, Wang J, Jen WP, Wu YJ, Chen YL. 2008. In search of
antioxidants and anti­atherosclerotic agents from herbal medicines. Biofactors., 34, 147­
57.
2. Juo CG, Chiu DT, Shiao MS*. 2008. Liquid chromatography­mass spectrometry in
metabolite profiling. Biofactors., 34, 159­69.
3. Hou CJ, Tsai CH, Su CH, Wu YJ, Chen SJ, Chiu JJ, Shiao MS, Yeh HI. 2008. Diabetes
reduces aortic endothelial gap junctions in ApoE­deficient mice: simvastatin exacerbates
the reduction. J Histochem Cytochem., 56, 745­52.
4. Chen TY, Lin BC, Shiao MS, Pan BS. 2008. Lipid­lowering and LDL­oxidation
inhibitory effects of aqueous extract of freshwater clam (Corbicula fluminea)­­using

3. inhibitory effects of aqueous extract of freshwater clam (Corbicula fluminea)­­using
tilapia as an animal model. J Food Sci., 73, H148­54.
5. Chen HI, Kao SL, Tsai MH, Shiao MS, Jen CJ. 2009. Exercise training modulates the
effects of lipoproteins on acetylcholine­induced endothelial calcium signaling in rat
aortas. Exp Biol Med (Maywood)., 234, 323­31.
6. Yang TL, Lin FY, Chen YH, Chiu JJ, Shiao MS, Tsai CS, Lin SJ, Chen YL. 2011.
Salvianolic acid B inhibits low­density lipoprotein oxidation and neointimal hyperplasia
in endothelium­denuded hypercholesterolaemic rabbits. J Sci Food Agric., 91(1), 134­
41.
7. Chen YR, Huang HB, Lo CJ, Wang CC, Su CL, Liu HT, Shiao MS, Lin TH, Chen YC.
2011. Aβ(40) (L17A/F19A) mutant diminishes the aggregation and neurotoxicity of
Aβ(40). Biochem Biophys Res Commun., 405(1), 91­5.
8. Leu YL, Wang PH, Shiao MS, Ismail W, Chiang YR*. 2011. A novel testosterone
catabolic pathway in bacteria. J Bacteriol., 193(17), 4447­55.
9. Tang CH, Tsao PN, Chen CY, Shiao MS, Wang WH, Lin CY*. 2011.
Glycerophosphocholine molecular species profiling in the biological tissue using
UPLC/MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci., 879(22), 2095­106.
10. Cheng ML, Shiao MS, Chiu DT, Weng SF, Tang HY, Ho HY*. 2011. Biochemical
disorders associated with antiproliferative effect of dehydroepiandrosterone in hepatoma
cells as revealed by LC­based metabolomics. Biochem Pharmacol., 82(11), 1549­61.
11. Chen KH, Cheng ML, Jing YH, Chiu DT, Shiao MS*, Chen JK*. 2011. Resveratrol
ameliorates metabolic disorders and muscle wasting in streptozotocin­induced diabetic
rats. Am J Physiol Endocrinol Metab., 301(5), E853­63.
12. Wang CC, Huang HB, Tsay HJ, Shiao MS, Wu WJ, Cheng YC*, Lin TH*. 2012.
Characterization of Aβ aggregation mechanism probed by congo red. J Biomol Struct
Dyn., 30(2), 160­9.
13. Tsai WC, Hsu SD, Hsu CS, Lai TC, Chen SJ, Shen R, Huang Y, Chen HC, Lee CH, Tsai
TF, Hsu MT, Wu JC, Huang HD, Shiao MS, Hsiao M, Tsou AP*. 2012. MicroRNA­
122 plays a critical role in liver homeostasis and hepatocarcinogenesis. J Clin Invest.,
122(8), 2884­97.
14. Ho HY, Cheng ML, Shiao MS, Tsun­Yee Chiu D*. 2013. Characterization of Global
Metabolic Responses of G6PD­Deficient Hepatoma Cells to Diamide­Induced Oxidative
Stress. Free Radic Biol Med., 54, 71­84.
15. Huang CF, Mei­Ling Cheng ML, Fan CM, Hong CY, Shiao MS*. (in press).
Nicotinuric acid: A potential marker of metabolic syndrome through a metabolomics­
based approach. Diabetes Care, doi: 10.2337/dc12­1067, 1­3.
Address：10F, The First Medical Building
FAX：+886­3­2118700
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Email：angeline@mail.cgu.edu.tw