QIAGEN® Originals — Pure Plasmids, Genuine Kits - Learn more

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Plasmid DNA purification is one of the most commonly used methods in molecular biology. While being relatively simple to perform, purification of high-purity DNA is critical for reliable results in downstream applications. As the innovator of plasmid DNA purification kits, QIAGEN has consistently set standards by providing faster preps, higher throughput, more convenience, and superior DNA quality for stringent applications. Learn more about the different plasmid purification solutions from QIAGEN for your research.

Health & Medicine

Sample to Insight
QIAGEN®
Originals — Pure Plasmids, Genuine Kits
Discover more about QIAGEN plasmid purification at
www.qiagen.com/PlasmidRESCTR
DNA purity, yield and kit format
Quality grade Kit name Yield (max.)
Scale
(LB volume*)
96-well
(1.3 – 5 ml)
DNA yield Prep time
Mini
(1 – 5 ml)
DNA yield Prep time
Midi
(20 – 50 ml)
DNA yield Prep time
Maxi
(80 –150 ml)
DNA yield Prep time
Mega
(500 ml)
DNA yield Prep time
Giga
(2.5 l)
DNA yield Prep time
Advanced transfection EndoFree®
Plasmid Kit†
– – – – – – <500 μg 150 min <2.5 mg 220 min <10 mg 310 min
HiSpeed®
Plasmid EF Kit†
– – – – – – – – 2.5 mg 50 min 10 mg 50 min
Transfection QIAGEN®
Plasmid Plus Kitǂ
<50 μg 50 min/plate§
– – <250 μg 20 min <1 mg 20 min <2.5 mg 40 min <10 mg 50 min
HiSpeed®
Plasmid Kit – – – – <200 μg 45 min <750 μg 60 min – – – –
QIAfilter Plasmid Kit – – – – <100 μg 110 min <500 μg 120 min <2.5 mg 190 min <10 mg 280 min
QIAGEN®
Plasmid Kit – – <20 μg 80 min <100 μg 150 min <500 μg 160 min <2.5 mg 220 min <10 mg 320 min
Molecular biology QIAprep®
Spin Miniprep Kit¶
– – <20 μg** 30 min – – – – – – – –
CompactPrep®
Plasmid Kit – – – – <200 μg <20 min <750 μg <20 min – – – –
QIAprep®
96 Plus Miniprep Kit <50 μg 40 min/plate§
– – – – – – – – – –
QIAprep®
96 Turbo Miniprep Kit <20 μg <45 min/plate§
– – – – – – – – – –
Sequencing QuickLyse Miniprep Kit – – <15 μg 9 min – – – – – – – –
R.E.A.L.®
Prep 96 Plasmid Kit <30 μg††
70 min/plate – – – – – – – – – –
DirectPrep®
96 Miniprep Kit <4 μg 35 min/plate – – – – – – – – – –
* Culture volumes when using a high-copy vector †
EndoFree = <0.1 EU/μg DNA ǂ
EndoReduced = <1 EU/μg DNA §
Prep times for manual handling ¶
Automatable on the QIAcube®
** High-Yield protocol also available ††
1 μg BAC
Plasmid Mini
Spin-based
A. Gravity flow
• QIAGEN Plasmid Mini Kits
B. Centrifuge-driven
• QuickLyse Miniprep Kits
C. Centrifuge- and vacuum-based on
QIAvac 24 Plus
• QIAprep Spin Miniprep Kits
Vacuum System
L. Vacuum system
• Vacuum pump
• QIAvac Connecting System connects to:
M. QIAvac 24 Plus
N. QIAvac HiSpeed LS
Plasmid Maxi
D. Gravity flow
• QIAGEN Plasmid Midi, Maxi, Mega and Giga Kits
• EndoFree Plasmid Maxi, Mega and Giga Kits
E. Gravity flow + QIAfilter/vacuum
• QIAfilter Plasmid Midi, Maxi, Mega and Giga Kits
F. Gravity flow + QIAfilter + QIAconcentrator
• HiSpeed Plasmid Midi and Maxi Kits
H. Vacuum-based on QIAvac HiSpeed LS
• HiSpeed Plasmid Mega Giga EF Kits
G. Vacuum-based on QIAvac 24 Plus
• CompactPrep Midi and Maxi Kits
• QIAGEN Plasmid Plus Midi, Maxi, Mega and Giga Kits
96-well plates
I. Centrifuge-driven
• QIAGEN Plasmid Plus 96 Miniprep Kits
• QIAprep 96 Plus Miniprep Kits
• R.E.A.L. Prep 96 Plasmid Kits
J. Vacuum-based on QIAvac 96
• QIAGEN Plasmid Plus 96 Miniprep Kits
• QIAprep 96 Plus Miniprep Kits
• QIAprep 96 Turbo Miniprep Kits
• R.E.A.L. Prep 96 Plasmid Kits
K. Vacuum-based on QIAvac Multiwell
• DirectPrep 96 Miniprep Kits
A B C
D E F
G H
I J K
L M N
110556302/2017
For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Services or your local distributor.
Trademarks: QIAGEN®
, Sample to Insight®
, QIAcube®
, QIAprep®
, EndoFree®
, HiSpeed®
, R.E.A.L.®
, DirectPrep®
, CompactPrep®
(QIAGEN Group). PROM-10577-001 © 2017 QIAGEN, all rights reserved.
Ordering www.qiagen.com/shop | Technical Support support.qiagen.com | Website www.qiagen.com

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