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1. This document consists of 16 printed pages and 2 blank pages.
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BIOLOGY 0610/04
Paper 4 Theory (Extended) For Examination from 2016
SPECIMEN PAPER
1 hour 15 minutes
Candidates answer on the Question Paper.
No Additional Materials are required.
READ THESE INSTRUCTIONS FIRST
Write your Centre number, candidate number and name on all the work you hand in.
Write in dark blue or black pen.
You may use an HB pencil for any diagrams, graphs or rough working.
Do not use staples, paper clips, glue or correction fluid.
DO NOT WRITE IN ANY BARCODES.
Answer all questions.
Electronic calculators may be used.
You may lose marks if you do not show your working or if you do not use appropriate units.
At the end of the examination, fasten all your work securely together.
The number of marks is given in brackets [ ] at the end of each question or part question.
Cambridge International Examinations
Cambridge International General Certificate of Secondary Education
The syllabus is accredited for use in England, Wales and Northern Ireland as a Cambridge International Level 1/Level 2 Certificate.

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1 The dominant grass species in an African grassland ecosystem are star grass and red oat grass.
• Star grass is eaten by antelope species, such as topi and Thomson’s gazelle.
• Smaller animals such as mice and grasshoppers feed on red oat grass.
• Topi and Thomson’s gazelle are eaten by predators such as cheetahs, lions and serval cats.
• Grasshoppers and mice are eaten by serval cats and tawny eagles.
• Vultures feed on dead mammals.
Fig. 1.1 shows part of the food web for this ecosystem.
star grass red oat grass
topi
Thomson’s
gazelle .................................
grasshoppers
lions
.................................
.................................
serval cats tawny eagles
Fig. 1.1
(a) Complete the food web by writing the names of the correct organisms in the boxes in Fig. 1.1.
[3]
(b) Name the trophic level of the following species.
star grass ...............................................................................................................................
topi ......................................................................................................................... [2]
(c) (i) State the principal source of energy for the food web shown in Fig. 1.1.
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(ii) State what happens to energy when it leaves a food web.
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(d) Explain why there are no more than four trophic levels in the food web shown in Fig. 1.1.
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(e) Fish such as salmon can be reared intensively in fish farms. They are fed on high protein food
made from animals. When eating this food, the fish are feeding as secondary consumers.
Describe the disadvantages of intensive farming, such as salmon farming, for producing
human food.
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[Total: 13]

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2 Fig. 2.1 shows a person sitting in a room. A thermometer shows the temperature of the room.
50
40
30
20
10
0
°C
Fig. 2.1
(a) Give three uses of energy in the body of the person in Fig. 2.1.
1 ...........................................................................................................................................
2 ...........................................................................................................................................
3 ..................................................................................................................................... [3]
(b) (i) Name the process carried out by the person in Fig. 2.1 that releases energy.
...................................................................................................................................... [1]
(ii) State the balanced chemical equation that describes this process.
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(c) The person leaves the room and runs very fast for 200m. When the person stops running, his
breathing rate and his heart rate remain high for several minutes.
Explain why the person’s breathing rate and heart rate remain high.
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(d) The run results in changes in the skin involving the blood vessels and the sweat glands.
Describe what happens to the blood vessels and sweat glands during the run.
Explain why these changes happen.
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[Total: 14]

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3 (a) Define the term sensitivity.
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Fig. 3.1 shows the reflex arc involved in a simple reflex action.
spinal nerve
spinal cord
Fig 3.1
(b) On Fig. 3.1 use label lines to identify and name the three types of neurone shown. [3]

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(c) A reflex is an involuntary action.
Explain what is meant by the term involuntary action.
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(d) (i) Define the term synapse.
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(ii) Describe how impulses are transmitted across a synapse.
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(e) When a body senses danger, more adrenaline is secreted from the adrenal glands.
Describe two ways in which the hormone adrenaline affects the body in preparation for action.
1 ................................................................................................................................................
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2 ................................................................................................................................................
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(f) State one difference between nervous and hormonal control systems.
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[Total: 13]

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4 The four o’clock plant, Mirabilis jalapa, can have flowers of three different colours as shown in
Fig. 4.1.
yellow
flower
red flower orange flower
Fig. 4.1
(a) A student crossed some red-flowered plants with some yellow-flowered plants (cross 1).
She collected the seeds and grew them. All of the plants that grew from these seeds had
orange flowers.
Complete the genetic diagram to explain the result of cross 1.
parental phenotypes
parental genotypes
gametes
offspring genotype
offspring phenotype
red flowers
RR
..............
×
×
+
yellow flowers
YY
..............
........................
........................
[3]

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(b) The student then carried out three further crosses as shown in Table 4.1.
Table 4.1
genotypes of offspring
cross 2 offspring of cross 1 × offspring of cross 1
cross 3 offspring of cross 1 × red-flowered plant
cross 4 offspring of cross 1 × yellow-flowered plant
Complete the table by writing in the genotypes of the offspring of crosses 2, 3 and 4, using
the same symbols as in the genetic diagram in (a).
You may use the space below for any working.
[3]
(c) Flower colour in M. jalapa is not an example of the inheritance of dominant and recessive
alleles.
Explain how the results of the crosses show that these alleles for flower colour are not dominant
or recessive.
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(d) The flowers from M. jalapa were cross-pollinated.
Explain the difference between self-pollination and cross-pollination.
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(e) Some plant species are self-pollinated.
Discuss the long-term effects of self-pollination on the evolution of these plant species.
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[Total: 15]

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5 Penicillin is an antibiotic produced by the fungus Penicillium chrysogenum.
Fig. 5.1 shows the process used to produce penicillin.
pH monitor
temperature
monitor
acid or alkali
fungus and
nutrients
filtrate
containing
penicillin
residue
waste
gases
sterile air
cooling water in
cooling water out
stirring
paddles
filtration
grid
water jacket
fermenter filter
Fig. 5.1

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(a) Explain why there is a water jacket around the fermenter and why acids or alkalis are added
to the fermenter.
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(b) Fig. 5.2 shows the mass of fungus and the yield of penicillin during the fermentation process.
0 20 40 60 80
time/hours
100 120 140 160
50
45
40
35
30
25
20
15
10
5
0
10
9
8
7
6
5
4
3
2
1
0
mass of
fungus
/g per dm3
yield of
penicillin
/arbitrary
units
nutrients added
penicillinpenicillin
fungusfungus
penicillin
fungus
Fig. 5.2
(i) State the time interval over which the fungus grew at its maximum rate.
................................................. hours [1]
(ii) As the fungus grows in the fermenter, the nuclei in the fungal hyphae divide.
State the type of nuclear division that occurs during the growth of the fungus in the
fermenter.
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(iii) Explain why the growth of the fungus slows down and stops.
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(c) Penicillin is not needed for the growth of P. chrysogenum.
(i) State the evidence from Fig. 5.2 that shows that penicillin is not needed for this growth.
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(ii) The people in charge of the penicillin production emptied the fermenter after 160 hours.
Use the information in Fig. 5.2 to suggest why they did not allow the fermentation to
continue for longer.
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(d) Downstream processing refers to all the processes that occur to the contents of the fermenter
after it is emptied. This involves making penicillin into a form that can be used as a medicine.
Suggest why downstream processing is necessary.
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(e) Explain why antibiotics, such as penicillin, affect bacteria but not viruses.
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[Total: 19]

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6 Fig. 6.1 shows a villus from the small intestine of a mammal and an enlarged view of a cell from
region A.
A
B
C
mitochondria
microvilli
magnification ×110 magnification ×2600
Fig. 6.1
(a) Name regions A, B and C.
A ..........................................................................................................................................
B ..........................................................................................................................................
C .................................................................................................................................... [3]
(b) Explain why the cells from region A have many microvilli and many mitochondria.
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[Total: 6]

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