POR PAULA ARROYAVE Y MARIA CAMILA ARUACHAN

1. Presentado por: Paula Andrea Arroyave Ramírez
María Camila Aruachán Barguíl
Facultad de Medicina
UPB - Medellín

2. Introduction
Is a gram-positive group B
Streptococcus (GBS)
¿WHAT IS IT?
In neonates it causes
sepsis, pneumonia and
meningitis
SYMPTOMS

3. Sample´s
extraction

4. Objective
Developed a visual MCDA assay
for S. agalactiae targeting pcsB
gene, which can achieve rapid,
sensitive and specific detection
of the target pathogen

5. DNAExtraction
Itisaprocessperformedto
collectgeneticmaterial(DNA)
forsubsequentmolecular
analysisorforensicanalysis.

6. Sensitivity
the ability of the test to
detect disease
Specificity
the probability that the test
will be negative if disease is
not present

7. it is a set of techniques that
allow the
maintenance of cells in vitro
Culture

8. PCR
PCRisatestusedtoenzymatically
amplifyaDNAsequenceandcan
helpestablishadiagnosis.

9. Results...

10. Results...

11. Results...

12. Results...

13. AUTHOR
Carrillo-Avilaetal.2018
Phillipsetal.1980;Elbehiryet
al.2015
Wangetal.2018;Gongetal.
2019;Lietal.2020
ARGUMENT
Asaresult,theincidenceofneonatal
infectedwithS.agalactiaehas
dropedmorethan60%
However,3.8%ofclinicalisolates
didn’tharborcfbgeneand9.31%of
ofclinicalisolatesdidn’thavesip
gene,whichmayleadingfalse-
negativeresults.Moreover,cfb
sequencesofS.pyogenes,S.uberis
andS.iniaehavemorethan70%
sequencesimilaritytothatofS.
agalactiae,whichmayleadtofalse-
positiveresults
MCDAproductswereusually
analyzedbyturbiditymeter,
intercalatingdyes,agarosegel
electrophoresisandLFB
AGREE/DISAGREE
x
Discussion

14. Onadailybasis,molecularbiologyhas
becomeveryimportantinclinical
importancebecauseitprovidesalternative
routesforthetreatmentofpathologiesthat
cansavealifewithonlytheearlydetection
ofthese,asistheexampleinthearticleof
thisinvestigation.
Conclusions
Molecularbiologytestshaveguaranteed
earlydiagnosis,whichcanleadtoeffective
andimmediatetreatmenttoobtaingood
results.
Molecularbiologyallowsthe
deepstudyofmicroorganisms
thatcanbepathogenicand
deadlyevenbeforetheyare
born,forwhichbiologyprovides
apreventivewaytobe
prepared.

15. ¡Muchas
¡Muchas
¡Muchas
gracias!
gracias!
gracias!