Presentation of 11 impact factor paper!

1. Presentation by: S. Jaishree
(Nov. 2/2022) PhD Student/BMS
Advisor: Prof. Jongkeun Seon
Impact factor
11.60

2. Osteoarthritis

3. Friction involved in joints

4. Pathology of Osteoarthritis

5. Treatment
strategies
Microfracture
A
C
I
MSC Therapy
(Cell based therapy)
Need for open
surgery
&
Long recovery
period
Chondrocyte
Dedifferentiation
&
Morbidity at the
donor site
Bioink
properties
&
Degradation
time
Dedifferentiation
&
Tumorigenicity
Scaffolds
Exosomal Therapy
(Cell free therapy)

6. To explore the therapeutic effect of MSC exosomes in a three dimensional (3D) printed scaffold for early
OA therapeutics.
Objective

7. 1. Exosomes isolation and identification
2. Preparation of the 3D printed, radially oriented ECM/GelMA/exosome bioink
3. Osteochondral defect models
4. Assessment of chondrocyte mitochondria
Materials & Methods

8. Results
1) Energy metabolism imbalance of degenerative cartilage

9. 2) Mitochondrial dysfunction in degenerative cartilage rescued by
exosomes

10. 3) In vivo analysis of ECM / GelMA / Exosomes scaffold

11. 4) Simulation M2 polarization (Immune response)

12. 5) Evaluation of cartilage regenerative capacity

13. 6) Final Evaluation of regenerated cartilage

14. • μCT reconstruction and histomorphological analysis of the osteochondral defect regions

15. Conclusion

16. Thankyou

18. Sample Containing Exosomes
ULTRACENTRIFUGATION
100,000*g for 1hour
Exosomes Sedimentation Pellet Containing Exosomes
TANGENTIAL FLOW FILTRATION
Sample Containing Exosomes
Filter by flow through them
Medium containing <30nm
Sample Containing Exosomes
SIZE EXCLUSION
CHROMATOGRAPHY
Exosome Excluded from Chromatography
IMMUNOAFFINTIY CAPTURE
Capture antibody
Magnetic Beads
Exosome capture on Magnetic beads
Exosome detection