Healthcare R2 B

1. New Castle Disease – R2B
Strain
Presented By:-
Dr. Tushar Dangat

2. Family - Paramyxoviridae
Genus - Avulavirus
9 serotypes APMV-1 to APMV-9
Newcastle disease is APMV-1
Newcastle Disease

3. Four pathotypes:
Asymptomatic enteric – Subclinical
Lentogenic
Subclinical to mild respiratory
Mesogenic
Respiratory or neurological
Velogenic
Neurotropic: Respiratory or neurological
Vicerotropic: Hemorrhagic intestinal lesions
Newcastle Disease

4. Morbidity: Up to 100%
Mortality: 90%
Varies greatly depending on
Virulence and strain
Susceptibility of host
Environmental conditions
Geographical area
Secondary infections
Vaccination history
NDV - Morbidity/Mortality

5. Edema of head, especially around eyes
Greenish-dark watery diarrhea
Respiratory and neurological signs
Signs vary with virulence of virus
High morbidity and mortality
Drop in egg production
Decrease Body wt. gain
Numerous deaths within 24-48 hours
Deaths toll continue for 7-10 days
Clinical Signs of ND

6. Indistinguishable from highly pathogenic AI
Hemorrhagic internal lesions
Tracheal mucosa
Proventriculus
Intestinal mucosa
Edema of the head and neck
Hemorrhage, necrosis or ulceration of lymphoid tissue
Lesions vary with strains & virulence
PM Lesions of - NDV

7. Pathogenecity Indices for strains of Newcastle Disease Virus
Virus Strain Pathotype ICPI IVPI MDT
Ulster 2C Lentogenic 0 0 >150
Queensland V4 Lentogenic 0 0 >150
Hitchner b1 Lentogenic 0.2 0 120
F Lentogenic 0.25 0 119
LaSota Lentogenic 0.4 0 103
H Mesogenic 1.2 0 48
Mukteshwar Mesogenic 1.4 0 46
Roakin Mesogenic 1.45 0 68
Beaudette C Mesogenic 1.6 1.45 62
GB Texas Velogenic 1.75 2.7 53
NY Parrot 70181 1972 Velogenic 1.8 2.6 51
Italien Velogenic 1.85 2.8 50
Milano Velogenic 1.9 2.8 50
Herts 33/56 Velogenic 2 2.7 48
NDV - Pathotypes

8. NDV – Mesogenic strain Mukteshwar strain
Each Dose of vaccine contains ND (R2B)vaccine
virus > 105EID50
Ventri’s R2B strain

9. Manjushree Pf - A/p Vadhu Bk.
Titers 0 2 4 8 16 32 64 128 256 512 1024 2048
weeks
6th week 1 1 4 5 9 2
8th week 13 4 3
9th weeek 1 4 6 5 3
10th week 1 2 3 5 1 5 2 1
12th week 1 1 6 6 2 1 1
13th week 2 2 6 6 3 1
14th week 4 5 2 6 3
15th week 1 2 1 6 3 5 2
Ventri’s R2B strain
Vaccination schedule –
LaSota, IBD std.- 5th day,
Hvt + SB1 – 13th day, IBD Plus – 21th day,
IB – 25th & 80th day, LaSota – 29st & 56th day,
R2B & Fowl Pox – 62th day,

10. Mandhare Pf - A/p Karnaudi Bk.
Titers 0 2 4 8 16 32 64 128 256 512 1024 2048
weeks
6th week 2 5 6 6 3
8th week 10 4 4 1 1
9th weeek 1 4 6 3
10th week 1 2 3 5 1 5 2 1
12th week 1 3 5 3 4
13th week 3 2 6 6 3 1
14th week 5 2 6 5 2
15th week 1 4 4 3 1 7
Ventri’s R2B strain
Vaccination schedule –
LaSota, IBD std.- 5th day,
Hvt + SB1 – 11th day, IBD Plus – 18th day,
IB – 24th & 78th day, LaSota – 31st & 54th day,
R2B & Fowl Pox – 70th day,

11. Observations :–
After giving LaSota (lentogenic strain)
vaccination with R2B (Mesogenic strain)
gives high and uniform antibody titers.
Ventri’s R2B strain
Referance –
J. Bangladesh Agril. Univ. 7(2): 329–334, 2009 ISSN 1810-3030
Determination of immune response of Newcastle disease virus
vaccines in layer chickens
N. A. Banu, M. S. Islam, M. M. H. Chowdhury and M. A. Islam
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-
2202, Bangladesh