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Koicarp is potentially an important cultured ornamental fish in freshwater. Moreover there were reports existing on genetic manipulation of koicarp by application of the heat shock. Hence the present study was made to contribute a protocol for induction of tetraploidy by heat shock in the koicarp.Induction of tetraploidy was attempted in Cyprinus carpio L, Koicarp by heat shock. Eggs from five females and milt from five males ok Koicarp were pooled to ensure the required quantity and quality of gametes for fertilization. After insemination the eggs were divided into three batches each experiment based on the post fertilization viz., 25min, 27min and 30min after insemination. Batches of eggs held in plastic containers were exposed to hot water at 38° C, 39° C, 40° C & 41° C for durations of 2min and four min. One batch of the eggs without heat shock treatment was used as control. After treatments, eggs were immediately transferred to incubation troughs. Tetraploidy was ascertained by karyotyping as well as RBC nuclear micro measurements.Heat shock of 41°C for four min, imparted to eggs for 20 min after fertilization induced a maximum of 60± 2% tetraploidy and maximum hatchability of 10± 1.5%. A large proportion of the heat shocked embryos displayed morphological abnormalities such as short and curved tail, destroyed yolksac, deformed vertebral column and malformed cephalic region. A maximum of 60± 2% tetraploids (4n = 156) were obtained when the fertilized eggs (20 min old) were heat shocked at 41° C for four min duration. The tetraploid red blood cells (RBCs) nucleus volume was 2.1 times greater than those of the diploid RBC nucleus.Given that koicarp are such a useful model for other areas of research, perhaps further studies on the induction of tetraploidy in this species will lead to a better understanding of polyploidy induction and the establishment of tetraploid lines of koicarp and other species as well.
Induction of tetraploidy in an ornamental fish koicarp Cyprinus carpio L, us...
Induction of tetraploidy in an ornamental fish koicarp Cyprinus carpio L, us...
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Koicarp is potentially an important cultured ornamental fish in freshwater. Moreover there were reports existing on genetic manipulation of koicarp by application of the heat shock. Hence the present study was made to contribute a protocol for induction of tetraploidy by heat shock in the koicarp.Induction of tetraploidy was attempted in Cyprinus carpio L, Koicarp by heat shock. Eggs from five females and milt from five males ok Koicarp were pooled to ensure the required quantity and quality of gametes for fertilization. After insemination the eggs were divided into three batches each experiment based on the post fertilization viz., 25min, 27min and 30min after insemination. Batches of eggs held in plastic containers were exposed to hot water at 38° C, 39° C, 40° C & 41° C for durations of 2min and four min. One batch of the eggs without heat shock treatment was used as control. After treatments, eggs were immediately transferred to incubation troughs. Tetraploidy was ascertained by karyotyping as well as RBC nuclear micro measurements.Heat shock of 41°C for four min, imparted to eggs for 20 min after fertilization induced a maximum of 60± 2% tetraploidy and maximum hatchability of 10± 1.5%. A large proportion of the heat shocked embryos displayed morphological abnormalities such as short and curved tail, destroyed yolksac, deformed vertebral column and malformed cephalic region. A maximum of 60± 2% tetraploids (4n = 156) were obtained when the fertilized eggs (20 min old) were heat shocked at 41° C for four min duration. The tetraploid red blood cells (RBCs) nucleus volume was 2.1 times greater than those of the diploid RBC nucleus.Given that koicarp are such a useful model for other areas of research, perhaps further studies on the induction of tetraploidy in this species will lead to a better understanding of polyploidy induction and the establishment of tetraploid lines of koicarp and other species as well.
Induction of tetraploidy in an ornamental fish koicarp Cyprinus carpio L, us...
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There have been mixed reactions to Danuna Tillakaratne’s foray into the Sri Lankan political arena. When questioned about this at a recent media interview, he clarified his position, explaining the reasons behind his decision.
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There have been mixed reactions to Danuna Tillakaratne’s foray into the Sri Lankan political arena. When questioned about this at a recent media interview, he clarified his position, explaining the reasons behind his decision.
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There have been mixed reactions to Danuna Tillakaratne’s foray into the Sri Lankan political arena. When questioned about this at a recent media interview, he clarified his position, explaining the reasons behind his decision.
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There have been mixed reactions to Danuna Tillakaratne’s foray into the Sri Lankan political arena. When questioned about this at a recent media interview, he clarified his position, explaining the reasons behind his decision.
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There have been mixed reactions to Danuna Tillakaratne’s foray into the Sri Lankan political arena. When questioned about this at a recent media interview, he clarified his position, explaining the reasons behind his decision.
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Though it was commonly claimed by mahinda rajapakse
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Fast Forward towards July 2015 with the Rajapakses no longer in power, and we see that General (now Field Marshal) Sarath Fonseka and Danuna Tillakaratne are still campaigning to create a better Sri Lanka free from corruption. They are both contesting for the General Elections representing the Democratic Party from the COlombo District. Danuna Tillakaratne played an integral role in the General's Presidential Election campaign in 2010 and also has played an important, albeit silent role in furthering the political cause of the Democratic party since the party's inception. He too is committed to eradicate corruption, establish good governance and bring about economic prosperity to the motherland.
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There have been mixed reactions to Danuna Tillakaratne’s foray into the Sri Lankan political arena. When questioned about this at a recent media interview, he clarified his position, explaining the reasons behind his decision.
Why Did Danuna Tillakaratne Enter Politics ?
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Colombo
There have been mixed reactions to Danuna Tillakaratne’s foray into the Sri Lankan political arena. When questioned about this at a recent media interview, he clarified his position, explaining the reasons behind his decision.
Sri Lanka Colombo Election 2015
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There have been mixed reactions to Danuna Tillakaratne’s foray into the Sri Lankan political arena. When questioned about this at a recent media interview, he clarified his position, explaining the reasons behind his decision.
Sri Lanka 2015 Election
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There have been mixed reactions to Danuna Tillakaratne’s foray into the Sri Lankan political arena. When questioned about this at a recent media interview, he clarified his position, explaining the reasons behind his decision.
Presidential Election 2015 Sri Lanka
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There have been mixed reactions to Danuna Tillakaratne’s foray into the Sri Lankan political arena. When questioned about this at a recent media interview, he clarified his position, explaining the reasons behind his decision.
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Though it was commonly claimed by mahinda rajapakse
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Colombo
Fast Forward towards July 2015 with the Rajapakses no longer in power, and we see that General (now Field Marshal) Sarath Fonseka and Danuna Tillakaratne are still campaigning to create a better Sri Lanka free from corruption. They are both contesting for the General Elections representing the Democratic Party from the COlombo District. Danuna Tillakaratne played an integral role in the General's Presidential Election campaign in 2010 and also has played an important, albeit silent role in furthering the political cause of the Democratic party since the party's inception. He too is committed to eradicate corruption, establish good governance and bring about economic prosperity to the motherland.
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Fast Forward towards July 2015 with the Rajapakses no longer in power, and we see that General (now Field Marshal) Sarath Fonseka and Danuna Tillakaratne are still campaigning to create a better Sri Lanka free from corruption. They are both contesting for the General Elections representing the Democratic Party from the COlombo District. Danuna Tillakaratne played an integral role in the General's Presidential Election campaign in 2010 and also has played an important, albeit silent role in furthering the political cause of the Democratic party since the party's inception. He too is committed to eradicate corruption, establish good governance and bring about economic prosperity to the motherland.
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Colombo
Fast Forward towards July 2015 with the Rajapakses no longer in power, and we see that General (now Field Marshal) Sarath Fonseka and Danuna Tillakaratne are still campaigning to create a better Sri Lanka free from corruption. They are both contesting for the General Elections representing the Democratic Party from the COlombo District. Danuna Tillakaratne played an integral role in the General's Presidential Election campaign in 2010 and also has played an important, albeit silent role in furthering the political cause of the Democratic party since the party's inception. He too is committed to eradicate corruption, establish good governance and bring about economic prosperity to the motherland.
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The entire family of Danuna was followed 24 hours a day for 7 to 8 months. All these efforts failed. No matter how hard they hit Danuna, he stood strong and finally in 2015, joined Field Marshal Fonseka to further his political cause.
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Even Danuna’s 80 year old aunt was not spared. She was taken in to CID custody for allegedly giving accommodation to Danuna. After going to courts for 3 years, she was acquitted and discharged of all accusations.
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False charges were leveled against him and were to be brought in to custody, where he would have been even tortured to implicate Mr.Fonseka. No matter, how much they tried, expending so much resources, they were not able to capture Danuna. However, Danuna lost 5 years of his life, which distanced him from his family and loved ones.
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Even Danuna’s 80 year old aunt was not spared. She was taken in to CID custody for allegedly giving accommodation to Danuna. After going to courts for 3 years, she was acquitted and discharged of all accusations.
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This situation is particularly embarrassing for the Secretary of Defense, Mr. Gotabhaya Rajapakse, who is personally overseeing the efforts to capture Danuna, and who has allocated several hundred personnel from the armed forces and police to the task of capturing Danuna.
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Danuna Evades Capture Although there has been a massive manhunt for Danuna Tillakaratne, the son in law of General Sarath Fonseka, who went into hiding following threats made to his life by the current regime, Danuna has so far been successful in evading capture.
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Like Field Marshal Sarath Fonseka, I too believe Sri Lanka needs change. But as we all know, during elections, everybody talks about change. However, if you really look at the situation, it is clearly apparent that the main political parties are not ready for real change. This is why we have to think seriously and be very selective about how we cast out valuable vote. We need to identify people who can create positive change, taking into account their experience backgrounds.
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