مع تحيات غدير الخفاجي

1. Test for coagulation disorders
• Thromboplastin + calcium + prothrombin Thrombin
Thrombin + Fibrinogen Fibrin ( clot)
Coagulation time :
A. Lee & White method
1. 3ml of blood in sterile syringe with needle washing
with physiological saline , record the time and place
each 1ml in tube then tilt the 1st & 2nd tubes at 30
second intervals until coagulation occurred then 3rd
tube in same manner , the elapsed time between
the first appearance of blood in syrange and clot
formation in the 3rd tube . ( most accurate )

2. • B. Capillary tube method :
1. Puncture the skin & fill special capillary tube with
blood , noting the time when blood first appears .
2. Holding the tube between the thumb and index
finger of both hands , gently break the tube every
30second until strand of fibrin is seen
C. Slid method :

3. Bleeding time
• Make small Deep puncture in the skin with lancet
and note the time when blood appears .
• Remove the drope of blood with filter paper every 30
second ( not touch the skin )
Prothrombin Time : Oxalated plasma is cloted by the
addition of calcium in presence of excess
throboplastin , the time required for clotting of
plasma is directed related to speed of conversion of
prothrombin to thrombin and depend upon the level
of plasma prothrombin .

4. Prothrombin deficiency
• Coagulation time , bleeding time and
prothrombin time are prolonged , thrombocyte
are normal .
1. Liver disease : sever hepatocellulr damage
2. Vitamin K deficiency .