1. PBL PRESENTATION
PBL PRESENTATION
Tittle : ‘We are korean!, don’t kill me..’
Lecturer : Dr. Syakirah binti Samsuddin
2. GROUP MEMBERS
NAME MATRIC NUMBER
NORFAZREENA BINTI MA’AMOR D20101037526
NOR SYUHADA BINTI SAIFUL ANUAR D20101037510
NUR SYAKHILA BINTI ZAHARI D20101037423
NUR HAYATI BINTI YUSOFF D20101037419
NORFARRAHDILAH BINTI AMIR D20101037438
3. ..About the article..
• Almost naked and decomposed body of a young Asian women was found
hidden in a suitcase.
• She had been suffocated.
• Her name was Hyo Jung Jin, 21 years old and was studying French at the
University of Lyon.
• She did not return to university in October after a trip to London.
• The police linked Hyo Jung Jin’s death to that of In Hea Song, whose
almost naked body was found in a cupboard in a house in East London.
• The house that being visited by Hyo Jung Jin and the body of In Hea Song
founded belong to the same landlord.
• Tape used by the murder to bind around Hyo Jung Jin face and body was
the same tape found out at Kyo Soo Kim’s house and has Kyo Soo Kim’s
blood found on it.
4. • Matched blue paint from a bedroom and paint at the suitcase
hiding Hyo Jung Jin’ body.
• Blood found at all over Kyo Soo Kim’s room.
• Mobile phone recorded trace Kim Soo Kim at the same place
as the Hyo Jung Jin’s body dump.
• Toronto : orange paint found on the t-shirt and the tape used
around In Hea Song’s wrist.
• Further DNA test confirmed that both body found in the same
cupboard.
5. ..Idea..
• The murder is the same person because the way of the
murderer look similar.
• Kyo Soo Kim is a suspect.
• Further evidence founded support the assumption that
the suspect is the murder.
• The polices investigate more further.
6. ..Learning Issues..
• How the investigator can relate both murder cases to each
other?
• What is the process needed to be used by using DNA in order
to identify the body?
• What are the evidences can be related between Kyo Soo Kim
and the murderer?
7. ..Technique to process evidence..
Paint Analysis
Polymerase Chain Reaction
Gel electrophoresis
8. Paint Analysis
• A specialized technique called pyrolysis gas chromatography might
be used to determine its composition.
• Pyrolysis involves heating the sample until it turns into a vapor.
• This is then injected into a gas chromatograph that separates the
components.
• These can be identified by molecular weight using mass
spectrometry, which creates a chemical fingerprint that can be
compared to reference samples.
9. Polymerase Chain Reaction
• With PCR, any specific segment one target sequence within a
DNA sample can be copied many times (amplified)
completely in vitro.
• Require double-stranded DNA containing the target
sequence, a heat- resistant DNA polymerase, all four
nucleotide two 15 to 20-nucleotide DNA strand that serve as
primers. One primer is complementary to one end of the
target sequence on one trand, the second primer is
complementary to the other end of the sequence on the
other strand.
10. • First step is denaturation : the reaction mixture is heated to
denature ( separate ) the DNA strands.
• Second, coal to allow primers to form hydrogen bonds with
ends of target sequence.
• Then, extension DNA polymerase add nucleotide to 3’end of
each primer.
• Repeat the PCR.
11. Gel electrophoresis
• Gel electrophoresis is used for separating nucleic acid or
protein that differ in size, electrical charge or other physical
properties.
• DNA molecules are separated by gel electrophoresis in
restriction fragment analysis of both cloned gene and genomic
DNA.
• Technique : gel electrophoresis separate macromolecule on the
basis of their rate of movement through a polymeric gel in an
electric field.
• Each sample, a mixture of DNA molecule is placed in a separate
well near one end of a thin slab of gel. The gel is set into a
small plastic support and immersed in an aqueous solution in a
tray with electrode at each end.
12. • When the current is turned on, the negatively charged DNA
molecule move toward the positive electrode with shorter
molecule moving faster than longer one.
• Bands are shown here in blue but on an actual gel, the bands
would not be visible at this time.
• The blue paint from the bedroom at Kyo Soo Kim house
matched to the blue paint on the suitcase Hyo Jung Jin’s body
was found.
13. Conclusion
• During the investigation, all the evidence
pointed to the suspect, Kyo Soo Kim.
• So, in 2003, the judge found Kyo Soo Kim guilty
for the two murders and gave him a life
sentence.
