1. Identification of Raji Cells membrane proteins associated
with gC1qR
1.6
1.4
1.2
O.D. 405 nm
1
0.8
0.6
0.4
0.2
0
21
tin
in
io
id
P-
-B
av
C
O
R
pt
M
1q
tre
gC
-S
AP

2. Effect of MF-1 peptide on gC1qR rebinding Raji Cells
2.5
2
1.5
OD 405nm
n=2
1
0.5
0
Biotin-BSA+MF-1 Biotin- Biotin-gc1qR+SF- Biotin-gc1qR+SF-
gc1qR+MF-1 1 2

3. gC1qR binding to Raji Cells
y = 0.0377Ln(x) + 0.5025
0.7 R2 = 0.8087
0.6
0.5
O.D. 405nm
0.4
Series1
Log. (Series1)
0.3
0.2
0.1
0
0 5 10 15 20 25 30
ug/mL of gC1qR

4. Effect of MF-1 peptide on gC1qR rebinding Raji Cells
0.7
0.6
O.D. 405 nm
0.5
0.4
+ .1M NaCl
0.3
0.2
0.1
e
tid
-1
-2
-1
-1
F
F
p
F
F
0
e
M
M
M
S
p
+
+
+
+
R
o
R
R
n
A
q
q
q
1
1
1
S
+
C
C
C
B
R
g
g
g
q
d
d
d
d
te
1
te
te
te
C
la
la
g
la
la
y
y
d
y
y
tin
tin
tin
tin
te
io
la
io
io
io
B
tiy
B
B
B
io
B
•Raji cell conc 1.2X10^6
•Peptides in .1M NaCl in TBS at 50ug/mL conc