This document discusses sentinel lymph node biopsy procedures for malignant melanoma. It describes the Cochran method of bisecting lymph nodes along the central plane to maximize the cut surface area for analysis. Sections are stained with H&E and immunohistochemically for S-100 and HMB-45 to identify melanoma metastases. An alternative method involves slicing the lymph node at 2mm intervals to avoid identifying the central plane. Immunostaining can demonstrate metastatic melanoma cells in subcapsular sinuses of sentinel lymph nodes.
17. Cook, M G et al. J Clin Pathol 2008;61:897-902 Metastases of melanoma are said to occur close to the median plane of the sentinel lymph node and therefore bivalving, as shown, and embedding face down in the cassette are recommended. www.Oncopathology.blogspot.com Processing of Sentinel Lymph node in malignant melanoma
18.
19. S-100 immunostaining of a sentinel lymph node demonstrating metastatic cells in a subcapsular sinuse. HMB-45 staining showing metastatic melanoma cells within subcapsular sinuses of a sentinel lymph node. www.Oncopathology.blogspot.com Sentinel Lymph node in Malignant Melanoma
