Management of HIV Infection in  Resource Poor Settings  Accurate & Affordable Reagent Alternatives
HIV in India – Current Status <ul><li>HIV Prevalence rate in India 0.36%   </li></ul><ul><ul><li>2.5 Million HIV  Infectio...
ReaMetrix Solutions for CD4 Enumeration <ul><li>Dry-Tri T-STAT CD348 </li></ul><ul><li>3 color single tube reagent for ope...
ReaMetrix Assay Reagent Characteristics <ul><li>Methodology </li></ul><ul><li>Absolute CD4 testing by flow Cytometry – gol...
ReaMetrix Assay Reagent Characteristics <ul><li>Accurate & reproducible </li></ul><ul><li>Workshop approved antibody clone...
Dry-Tri T-STAT CD348  <ul><li>3 color single tube reagent </li></ul><ul><ul><li>Anti CD4 – Atto488* (FITC eq. dye) </li></...
Dry-Tri T-STAT CD3445 <ul><li>3 color single tube reagent </li></ul><ul><ul><li>Anti CD4 – Atto488* (FITC eq. dye) </li></...
ReaPan 34845 <ul><li>4  color single tube reagent,  </li></ul><ul><ul><li>Anti CD4 – APC  </li></ul></ul><ul><ul><li>Anti ...
Rea T-Count Reagent <ul><li>Rea T-count CD3/CD4 & CD3/CD8 re agent </li></ul><ul><ul><li>Designed for BD FACSCount flow Cy...
ReaPan Reagents for the Guava Platform <ul><li>ReaPan 34G </li></ul><ul><ul><li>2 color single tube reagent  </li></ul></u...
Summary… <ul><li>ReaMetrix supplies all reagents with ReaLyse, a buffer for lysing and fixing samples or ReaFix, a buffer ...
Manufactured by ReaMetrix India Pvt. Ltd. 50-B, II Phase, Peenya Industrial Area Peenya, Bangalore 560058, India Ph: +91-8...
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ReaMetrix Hiv Reagent Portfolio

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ReaMetrix HIV Reagents

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ReaMetrix Hiv Reagent Portfolio

  1. 1. Management of HIV Infection in Resource Poor Settings Accurate & Affordable Reagent Alternatives
  2. 2. HIV in India – Current Status <ul><li>HIV Prevalence rate in India 0.36% </li></ul><ul><ul><li>2.5 Million HIV Infections </li></ul></ul><ul><li>By year 2010, estimates indicate that there will be between 8-15 million HIV infected individuals </li></ul><ul><li>Management of HIV Infection </li></ul><ul><ul><li>Providing Anti Retroviral therapy (ART) </li></ul></ul><ul><ul><li>Immunophenotyping </li></ul></ul><ul><ul><ul><li>CD4 T-Lymphocyte Count </li></ul></ul></ul><ul><ul><li>Viral Load Assay – </li></ul></ul><ul><ul><ul><li>PCR – quantitate virus levels in blood </li></ul></ul></ul>CD4 counts are the single most important criteria to guide clinical decision-making on when to use ART
  3. 3. ReaMetrix Solutions for CD4 Enumeration <ul><li>Dry-Tri T-STAT CD348 </li></ul><ul><li>3 color single tube reagent for open flow Cytometers </li></ul><ul><li>eg. BD FACScan ™, BD FACSCalibur ™, BC EPICS XL™) </li></ul><ul><li>Dry-Tri T-STAT CD3445 </li></ul><ul><li>3 color single tube reagent for open flow Cytometers </li></ul><ul><li>(eg. BD FACScan™, BD FACSCalibur™, BC EPICS XL™) </li></ul><ul><li>ReaPan 34845 </li></ul><ul><li>4 color single tube reagent for open Flow Cytometers </li></ul><ul><li>(eg. BD FACSCalibur™) </li></ul><ul><li>Rea T count </li></ul><ul><li>2 color dual tube reagent for FACScount ™ Platform </li></ul><ul><li>ReaPan 34G-38G </li></ul><ul><li>2 Color dual tube reagent for the Guava™ Platform </li></ul>
  4. 4. ReaMetrix Assay Reagent Characteristics <ul><li>Methodology </li></ul><ul><li>Absolute CD4 testing by flow Cytometry – gold standard method </li></ul><ul><li>Employ simple Lyse/no wash protocol - single platform technology </li></ul><ul><li>Convenience </li></ul><ul><li>Provided as a ready to use format. No additional dispensing </li></ul><ul><li>Unitized form </li></ul><ul><li>Pre-dispensed reagents in unitized form – reduces pipetting errors </li></ul><ul><li>Stability & storage </li></ul><ul><li>Dried down reagents – do not require cold storage. No aggregation of </li></ul><ul><li>antibodies & beads in dried form </li></ul><ul><li>Simplified work flow </li></ul><ul><li>Minimal hand on time - just require sample pipetting, reduced steps </li></ul>
  5. 5. ReaMetrix Assay Reagent Characteristics <ul><li>Accurate & reproducible </li></ul><ul><li>Workshop approved antibody clones – provide excellent Analytical </li></ul><ul><li>Specificity & Analytical Sensitivity </li></ul><ul><li>Extensively validated reagents in various private & public labs in India </li></ul><ul><li>Sample stability </li></ul><ul><li>Processed samples can be stored for up to </li></ul><ul><li>7 days prior to analysis ( CV = 5.7% ). </li></ul><ul><li>Affordable </li></ul><ul><li>All of these attributes without compromising affordability </li></ul><ul><li>Technical support </li></ul><ul><li>ReaMetrix provides training on Cytometer and reagent </li></ul><ul><li>ReaMetrix provides technical support online & through personal visits </li></ul>
  6. 6. Dry-Tri T-STAT CD348 <ul><li>3 color single tube reagent </li></ul><ul><ul><li>Anti CD4 – Atto488* (FITC eq. dye) </li></ul></ul><ul><ul><li>Anti CD8 – R-PE </li></ul></ul><ul><ul><li>Anti CD3 – R-PE-Dy649* (Cy5 eq. dye) </li></ul></ul><ul><li>Provides absolute counts (Cells/µL) of CD3+, CD4+, CD8+ Cells & CD4/CD8 Ratio </li></ul><ul><li>Validated with commercial reagents at NARI, YRG Care </li></ul><ul><li>CE Marked reagent </li></ul>Reagents are suitable to run on open flow cytometers such as BD FACScan, BD FACSCalibur, Beckman Coulter EPICS-XL etc. Reagents supplied in Dry, Unitized Format * Dyes qualified by ReaMetrix to assure superior performance
  7. 7. Dry-Tri T-STAT CD3445 <ul><li>3 color single tube reagent </li></ul><ul><ul><li>Anti CD4 – Atto488* (FITC eq. dye) </li></ul></ul><ul><ul><li>Anti CD3 – R-PE-Dy649* (Cy5 eq. dye) </li></ul></ul><ul><ul><li>Anti CD45 – R-PE </li></ul></ul><ul><li>Provides a bsolute counts (Cells/µL) of Absolute Lymphocyte Count (ALC), CD3+, CD4+, % CD4 Counts </li></ul><ul><li>Validated with commercial reagents at leading path labs in India </li></ul><ul><li>Suited for aged blood samples </li></ul>Reagents are suitable to run on open flow cytometers such as BD FACScan, BD FACSCalibur, Beckman Coulter EPICS-XL etc. Reagents supplied in Dry, Unitized Format * Dyes qualified by ReaMetrix to assure superior performance
  8. 8. ReaPan 34845 <ul><li>4 color single tube reagent, </li></ul><ul><ul><li>Anti CD4 – APC </li></ul></ul><ul><ul><li>Anti CD8 – R-PE </li></ul></ul><ul><ul><li>Anti CD3 – Atto488* (FITC eq. dye) </li></ul></ul><ul><ul><li>Anti CD45 – PerCP </li></ul></ul><ul><li>Provides a bsolute counts (Cells/µL) of ALC, CD3+, CD4+, CD8+ Cells, % CD4 & CD4/CD8 Ratio </li></ul><ul><li>Validated with BD multiTEST 4 color reagent at leading path. Lab in India. </li></ul><ul><li>Suited for aged blood samples </li></ul><ul><li>Designed for BD FACSCalibur dual laser flow Cytometer </li></ul><ul><li>CE marked </li></ul>Reagents supplied in Dry, Unitized Format * Dyes qualified by ReaMetrix to assure superior performance
  9. 9. Rea T-Count Reagent <ul><li>Rea T-count CD3/CD4 & CD3/CD8 re agent </li></ul><ul><ul><li>Designed for BD FACSCount flow Cytometer </li></ul></ul><ul><ul><li>Available as 2 color dual tube reagent for </li></ul></ul><ul><ul><ul><li>CD3+, CD4+, CD8+, CD4/CD8 ratio estimation </li></ul></ul></ul><ul><ul><li>Available as 2 color single tube reagent for </li></ul></ul><ul><ul><ul><li>CD3+, CD4+ estimation </li></ul></ul></ul><ul><ul><li>Alternative for FACSCount reagent </li></ul></ul><ul><ul><li>Reagent validated at </li></ul></ul><ul><ul><ul><li>National HIV Immunology laboratory, Canada </li></ul></ul></ul><ul><ul><ul><li>Zimbabwe </li></ul></ul></ul>Reagents supplied in Dry, Unitized Format
  10. 10. ReaPan Reagents for the Guava Platform <ul><li>ReaPan 34G </li></ul><ul><ul><li>2 color single tube reagent </li></ul></ul><ul><ul><li>Provides absolute counts (Cells/µL) of CD3+, CD3+CD4+ counts </li></ul></ul><ul><li>ReaPan 34G & 38G </li></ul><ul><ul><li>2 color single dual reagent </li></ul></ul><ul><ul><li>Provides absolute counts (Cells/µL) of CD3+, CD3+CD4+ & CD3+CD8+ counts </li></ul></ul><ul><li>Validated with Guava Reagent at KIMS, Bangalore </li></ul>Reagents supplied in dry, unitized format
  11. 11. Summary… <ul><li>ReaMetrix supplies all reagents with ReaLyse, a buffer for lysing and fixing samples or ReaFix, a buffer for fixing samples after staining </li></ul><ul><li>ReaMetrix has the capability to develop CD4+ enumeration solutions for any flow cytometer platform. </li></ul><ul><li>ReaMetrix can customize solutions as per your need. </li></ul><ul><li>Work is in progress for reagent development for other platforms. </li></ul>
  12. 12. Manufactured by ReaMetrix India Pvt. Ltd. 50-B, II Phase, Peenya Industrial Area Peenya, Bangalore 560058, India Ph: +91-80-28378693/5, Fax: +91-80-41172451 E-mail: info@reametrix.com www.reametrix.com Further details:

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