2016 05-30-monday-assembly

Yannick Wurm
Yannick WurmReader in Evolutionary Genomics at Queen Mary, University of London
Spring-school
in Bioinformatics
& Population Genomics
(practical sessions)
http://bit.ly/genomicscourse
Wifi issues
• Reduce # devices unnecessarily connected:
• Turn off wifi
• Reduce bandwidth.When connected:
• turn off Dropbox, Google Drive, OneDrive, iTunes etc.
• Keep unnecessary browser windows closed
From reads to reference genome
& gene predictions.
1. inspect and clean short (Illumina) reads,
2. perform genome assembly,
3. assess the quality of the genome assembly using simple
statistics,
4. predict protein-coding genes,
5. assess quality of gene predictions,
6. assess quality of the entire process using a biologically
meaning measure.
vm password: sibuser
http://bit.ly/genomicscourse
2016 05-30-monday-assembly
It was the season of darkness, it was the
spring of hope, it was the winter of
despair, we had everything before us, we
had nothing before us, we were all going
direct to heaven, we were all going direct
the other way.
2016 05-30-monday-assembly
Sequencing technologies?
2016 05-30-monday-assembly
Assembly approaches?
2016 05-30-monday-assembly
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2016 05-30-monday-assembly

  • 1. Spring-school in Bioinformatics & Population Genomics (practical sessions) http://bit.ly/genomicscourse
  • 2. Wifi issues • Reduce # devices unnecessarily connected: • Turn off wifi • Reduce bandwidth.When connected: • turn off Dropbox, Google Drive, OneDrive, iTunes etc. • Keep unnecessary browser windows closed
  • 3. From reads to reference genome & gene predictions. 1. inspect and clean short (Illumina) reads, 2. perform genome assembly, 3. assess the quality of the genome assembly using simple statistics, 4. predict protein-coding genes, 5. assess quality of gene predictions, 6. assess quality of the entire process using a biologically meaning measure.
  • 7. It was the season of darkness, it was the spring of hope, it was the winter of despair, we had everything before us, we had nothing before us, we were all going direct to heaven, we were all going direct the other way.