005 panicle bacterial blight in colombia, gloria mosquera

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005 panicle bacterial blight in colombia, gloria mosquera

  1. 1. Panicle bacterial blight in Colombia:  g knowing the enemy as a first step in finding  control alternatives Gloria Mosquera, Plant Pathologist Rice Plant Pathology Program gy g International Center for Tropical Agriculture, CIAT Cali, Colombia
  2. 2. Presentation Outline •General Background • St di on di Studies disease cycle i greenhouse l in h •Identification of causal agent on field samples •Disease distribution and variability •Current activities and future work Current
  3. 3. General  Background Causal Agent Burkholderia glumae g A Symptoms: Grain discoloration, infertility Straight panicle  Straight panicle Sheath rot in seedlings (Mainly in nurseries, not frequent in  field) Reported in Colombia in 1987 (Zaigler and Alvarez)
  4. 4. Seed‐Born disease but… Not only infected seeds seem to be  required for disease development N l i f d d b i d f di d l Greenhouse observations Greenhouse observations Field conditions 3. Symptoms on flowers 3 S fl Plants without  (bacteria concentration  symptoms not  increased)  even in flowers 2. Epiphytic phase (low bacteria concentration) Infected  seeds 1.  Bacteria seed‐transmitted
  5. 5. 1 Identificación por PCR Disease widely  Depto. Localidad B.  2 (%) 3 B. gladioli 2 (%) 3 glumae distributed in Colombia distributed in Colombia Antioquia 1 2(1) 50 2(0) 0 Atlántico 1 1(0) 0 1(0) 0 B. gladioli has been also  B gladioli has been also Bolívar 2 4(3) 75 4(0) 0 detected. First report in  Casanare 1 1(0) 0 1(0) 0 Colombia. ( (Poster by P. Fory) y y) Cesar  3 17(12) 71 11(6) 55 CIAT 1 157(59) 38 68(10) 15 Córdoba 4 11(8) 73 11(0) 0 Huila 3 7(2) 29 6(1) 17 Meta 6 40(19) 48 26(3) 12 Santander 1 2(2) 100 1(0) 0 Sucre 1 5(2) 40 5(0) 0 Tolima 13 94(58) 62 50(8) 16 V. del  2 3(1) 33 3(1) 33 Cauca Total  39 344(167) 49 189(29) 15
  6. 6. B. glumae vs B gladioli B.glumae more aggressive B. gladioli less aggressive  B. gladioli less aggressive but similar symptoms
  7. 7. B. glumae is widely  distributed in Colombia  Cepa Registro Departame nto Municipio Variedad Pigmentación but how diverse is the  1 2 3161‐2 3193‐No 4 Sucre Córdoba San Marcos La victoria Fedearroz 2000 Fedearroz 2000 Pigmentada Pigmentada 3 3193‐No 5 Córdoba La victoria Fedearroz 2000 No Pigmentada population? 4 5 3196‐10 3200‐12 Meta Córdoba Santa Rosa La victoria Oryzica Llanos 5 Fedearroz 2000 No Pigmentada Pigmentada (Poster by D. Bravo) 6 3252‐ 8 Antioquia Nechi Desconocida Pigmentada 7 3455‐1 3455 1 Bolívar San Jacinto San Jacinto Fedearroz 733 Fedearroz 733 Pigmentada 8 3459‐4 Bolívar María la baja Fedearro473 Pigmentada Location where disease has  an effect  on yield  9 3643‐2 Córdoba Lorica    Arroz rojo Pigmentada 10 3646‐1 Córdoba Lorica    Fedearroz 2000 Pigmentada 11 3650‐3 Córdoba Lorica    Fedearroz 733 Pigmentada 12 3651‐1 Córdoba Montería 71 Pigmentada 13 3651‐4 Córdoba Montería Fedearroz 733 No Pigmentada 14 3656‐1 Córdoba Montería Mezc. Fedearroz Pigmentada 15 3659‐1 Córdoba Montería Fedearroz mejor. Pigmentada 16 3718‐2 Tolima Ambalema  Or‐370 Pigmentada 17 3722‐1 Tolima Guamo CimarronBarinas Pigmentada 18 3807‐9 Tolima Espinal Orizya 1 Pigmentada 19 3809‐2 Tolima Guamo Fedearroz 60 Pigmentada 20 3818 8 3818‐8 Tolima Venadillo Clearfield 370 Clearfield‐370 Pigmentada 21 3825‐14 Tolima Venadillo Fedearroz 60 Pigmentada 22 3847B‐10(S) Meta Santa Rosa Fedearroz 174 Pigmentada 23 3848‐5(S) Meta Santa Rosa Fedearroz 134 Pigmentada 24 3851‐4 Meta Santa Rosa 71 Pigmentada 25 3853‐4 Tolima Progreso  CimarronBarinas Pigmentada 26 3854‐2 Tolima Prado CimarronBarinas Pigmentada 27 3855‐4 Tolima Progreso  Progreso Coprosem 304 Coprosem 304 Pigmentada 28 3856‐5 Tolima Parificación CimarronBarinas Pigmentada 29 3858‐2 Tolima Parificación Coprosem 304 Pigmentada 30 3859‐5 Tolima Santa Inés CimarronBarinas No Pigmentada 31 3861‐2 Meta Santa Rosa RD‐FLAR 18 Pigmentada 32 3862‐3 Meta Santa Rosa RD‐FLAR 28 Pigmentada 33 3864‐4 Meta Santa Rosa RD‐FLAR 68 Pigmentada 34 3866B 10 3866B‐10 Santander Cúcuta Fedearroz 733 Fedearroz 733 Pigmentada 35 3880‐7 Cesar Tamalameque Fedearroz 2000 Pigmentada 36 3883‐8 Meta Villavicencio Improarroz 1550 Pigmentada 37 3889A‐2 Tolima Armero Panorama 20‐08 Pigmentada 38 3890‐4  Tolima Saldaña Coprosem 1 Pigmentada 39 3891A‐1 Tolima Ibagué Aceituno  Pigmentada
  8. 8. B. glumae population is highly diverse 5 main genetic clusters correlated with geographical BOX-PCR profiles location Eastern plains North N th Center Meta Mixed
  9. 9. Adjusting a system for strain aggressiveness/host response evaluation B. B glumae Water Panicle inoculation Colombia XXI highly susceptible Non pigmented strains as reference Evaluation after 14 days of inoculation
  10. 10. Establishment of evaluation system •Colombia XXI was susceptible to most of the strains •Pathogenic strains> 50% damage •Low pathogenic <20% damage The same system is being used to screen germplasm looking for tolerance sources Strains representing all genetic clusters are being included in this evaluation Tolerant materials (hot spot) will be challenged with multiple location strains.
  11. 11. OUR TEAM Gustavo Prado Paola Fory Girlena Aricapa Liliana Bolaños Carlos Prado Luis Rosero Fomer members Daniela Bravo OUR COLLABORATORS FEDEARROZ FLAR MADR

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