This modeling approach is being incorporated into a new generation of knowledge system at RoboSphere, Sigmoid, to extend the frontiers of a variety of kinomes. Sigmoid uses an innovative agent based technology, developed at RoboSphere. Sigmoid interactively assists researchers in the hypothesis and evaluation of potential signal transduction systems. Each Sigmoid knowledge agent maintains knowledge of an object, i.e. tissue, cell, kinase, ligand, gene, in a cell signaling environment. Each agent in turn maintains a local environment, i.e. cell environment, to maintain a catalog of constituent objects. Sigmoid provides a veritable silicon laboratory to explore the phenome expression of a variety of signal moieties and molar distributions.