Obtaining BloodCultures in the Infant NLC P/P# 6600.0212
Purpose: To detect bacterial infection of the blood in the infant.Policy: Alcohol swabs are never used for collecting bloodcultures, use antiseptic swabs only. Patient identification isconfirmed before the procedure is begun.
Equipment Antiseptic swabs Disposable gloves Tourniquet Adhesive bandage or adhesive tape, cotton balls or gauze pads Anaerobic and aerobic culture bottles Completed identification labels for blood tubes Plastic bag for delivery of specimen to laboratory Sterile needles: 23 or 25 gauge, or butterfly Sterile syringes: 3-5 ccs
Procedure:1. Perform hand hygiene.2. Place infant in position to facilitate blood draw.3. Put on gloves and Apply tourniquet above site selected.4. Cleanse venipuncture sites with antiseptic swabs, allow to dry on skin5. Collect at least one (1) ml of venous blood in 3-5 ml syringe from the venipuncture sites.6. Release tourniquet, Apply cotton ball or gauze pad over puncture site and withdraw needle, apply pressure over site until bleeding stops.Tape dressing securely.7. Discard needle on syringe; replace with new sterile needle before injecting blood sample into culture bottle.8. If aerobic and anaerobic cultures are needed, inoculate anaerobic first.9. Mix gently after inoculation,10. Label the specimen with infant’s name, date, time, and tentative diagnosis. Place in appropriate bag for transfer to lab.11. Order Blood Culture in CPSI and transport the culture bottles immediately to the laboratory.
Documentation:Document description of venipuncture procedure (includingsite, amount of specimen collected, time obtained, disposition ofspecimen, infant’s response to procedure).