DNA repair
Mutations
The Ames Test
The repair systems <ul><li>Mainly, the undamaged complementary strand acts as a template for the DNA polymerases involved ...
Mismatch repair
Mismatch mechanism
Mutations in mut proteins?
Base excision repair
Repairing apurinic and apyrimidinic sites
Nucleotide excision repair
Mutations in excision repair systems
 
Direct repair
Methyltransferase
When both strands are damaged
SOS system
Recombination
Recombination <ul><li>Three forms </li></ul><ul><ul><li>Homologous </li></ul></ul><ul><ul><li>Site specific recombination ...
Recombinational repair
DNA replication stalled because of lesion  Replication continues Repair Recombination Blockade
Replication following a break Blockade  Recombination Reseal
Meiotic recombination
Recombination between homologous  a  chromosomes
Recombination permits genetic mapping If each recombinant occurs at 2.5%, then the total map distance is 5% or 5 m.u. 1 2 ...
The simple minded mechanism (incorrect) of meiotic recombination
Gene conversion indicates that another mechanism must be operable
Gene conversion on a molecular level
The mechanism of homologous recombination
The topology of the Holliday structure
Resolution of the Holliday structure
Non-identical sequences
The enzymes of recombination
Rec A
Site specific recombination
Site specific recombination within one molecule
Use of recombinase to resolve a dimeric genome
Transposition
Direct transposition
Replicative transposition
Retroviruses
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Molbiol 2011-09-reparation-recombination

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Molbiol 2011-09-reparation-recombination

  1. 1. DNA repair
  2. 2. Mutations
  3. 3. The Ames Test
  4. 4. The repair systems <ul><li>Mainly, the undamaged complementary strand acts as a template for the DNA polymerases involved in repair </li></ul><ul><ul><li>Mismatch repair </li></ul></ul><ul><ul><li>Base excision repair </li></ul></ul><ul><ul><li>Nucleotide excision repair </li></ul></ul><ul><ul><li>Direct repair </li></ul></ul><ul><ul><li>Recombinational repair </li></ul></ul><ul><ul><li>Error-prone repair </li></ul></ul>
  5. 5. Mismatch repair
  6. 6. Mismatch mechanism
  7. 7. Mutations in mut proteins?
  8. 8. Base excision repair
  9. 9. Repairing apurinic and apyrimidinic sites
  10. 10. Nucleotide excision repair
  11. 11. Mutations in excision repair systems
  12. 13. Direct repair
  13. 14. Methyltransferase
  14. 15. When both strands are damaged
  15. 16. SOS system
  16. 17. Recombination
  17. 18. Recombination <ul><li>Three forms </li></ul><ul><ul><li>Homologous </li></ul></ul><ul><ul><li>Site specific recombination </li></ul></ul><ul><ul><li>Transposition </li></ul></ul>
  18. 19. Recombinational repair
  19. 20. DNA replication stalled because of lesion Replication continues Repair Recombination Blockade
  20. 21. Replication following a break Blockade Recombination Reseal
  21. 22. Meiotic recombination
  22. 23. Recombination between homologous a chromosomes
  23. 24. Recombination permits genetic mapping If each recombinant occurs at 2.5%, then the total map distance is 5% or 5 m.u. 1 2 3 Generation
  24. 25. The simple minded mechanism (incorrect) of meiotic recombination
  25. 26. Gene conversion indicates that another mechanism must be operable
  26. 27. Gene conversion on a molecular level
  27. 28. The mechanism of homologous recombination
  28. 29. The topology of the Holliday structure
  29. 30. Resolution of the Holliday structure
  30. 31. Non-identical sequences
  31. 32. The enzymes of recombination
  32. 33. Rec A
  33. 34. Site specific recombination
  34. 35. Site specific recombination within one molecule
  35. 36. Use of recombinase to resolve a dimeric genome
  36. 37. Transposition
  37. 38. Direct transposition
  38. 39. Replicative transposition
  39. 40. Retroviruses

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