Ai manual chapter 08


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Inseminacao Artificial em Bovinos ABS Global

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  • In this section we will explore the equipment you will need for an A.I. Program.
  • Let’s begin with the refrigerator, it is an extremely efficient piece. The construction is similar to that in a thermos. It has an inner chamber surrounded by insulation, and an outer shell made of aluminum. Then is vacuum drawn and sealed.
  • Refrigerators are sturdy pieces of equipment, but need to be handled properly when moving it, to reduce the risk of damage.
  • Always store in a well ventilated area, nitrogen displaces oxygen. Also liquid nitrogen is like boiling water and will burn the skin. Never store the refrigerator directly on concrete, as it causes electrolysis and can damage the aluminum outer shell.
  • Sanitation is a priority, so keep your refrigerator clean by washing with cold water and a mild detergent.
  • Liquid nitrogen can burn the skin, wear the proper clothing and safety equipment when handling it.
  • Should your stopper freeze, remove immediately by using a screwdriver to pry it out. (Be careful of the neck tube.) Keeping the stopper in place and the dustcover closed will reduce the chances of the stopper freezing.
  • Have a schedule to check your nitrogen level regularly, all refers will eventually fail, and this can prevent losing valuable semen.
  • Ultimately the responsibility to maintain and service the unit rests with you, the user.
  • Each refrigerator should have a tag that indicates the model number. This chart, which is also in your manual in Chapter 8 on page 4, can help determine the holding time.
  • You will also find a chart indicating expected nitrogen levels on page 8-4.
  • By keeping an ongoing record of your nitrogen measurements you can be proactive to any failures.
  • Monitor ampules are provided as a tool to help you protect your semen investment.
  • Check your monitor ampules after each use or opening of the refrigerator, read them in the neck tube.
  • When transferring semen wear proper safety equipment and do it quickly, it is a two-person job.
  • Keeping a record of your semen inventory is just good business. It eliminates hunting through all the canisters, unnecessarily exposing semen to risk of damage.
  • These are the key things to remember regarding your liquid nitrogen refrigerator.
  • Ai manual chapter 08

    1. 1. CHAPTER 8 <ul><li>REFRIGERATOR CARE & MAINTENANCE </li></ul>
    2. 2. How a Refrigerator Works <ul><li>Extremely Efficient Insulation System </li></ul><ul><ul><li>Airless </li></ul></ul><ul><ul><li>Aluminum Foil </li></ul></ul><ul><li>Maintains a -320  F Temperature </li></ul><ul><li>Specially Designed Neck Tube Stopper </li></ul>
    3. 3. Moving The Refrigerator <ul><li>Lift With Both Hands </li></ul><ul><li>Set Down Squarely and Gently </li></ul><ul><li>ALWAYS Secure for Transportation </li></ul><ul><li>DON’T Skid Across the Floor </li></ul><ul><li>DON’T Bang it Into Door Frames </li></ul><ul><li>DON’T Drop it on Edge </li></ul><ul><li>AVOID Dents and Scratches </li></ul>
    4. 4. Storing The Refrigerator <ul><li>Keep in a Clean, Dry, Well-Ventilated Area </li></ul><ul><li>Keep in Cool Spot Away From Direct Sunlight </li></ul><ul><li>Keep in Location Safe From Children </li></ul><ul><li>Keep in Location Where Can be Seen Often </li></ul><ul><li>DON’T Set Directly on Concrete </li></ul><ul><li>DON’T Fill With Liquid Nitrogen if Stored in a Box </li></ul>
    5. 5. Keep The Refrigerator Clean <ul><li>Wash with Cold Water and Mild Detergent </li></ul><ul><li>Rinse with Cold Water </li></ul><ul><li>AVOID Splashing Water on Neck Tube and Neck Tube Plug </li></ul>
    6. 6. Working With Liquid Nitrogen <ul><li>Always Wear Gloves and Safety Glasses </li></ul><ul><li>AVOID Splashing and Direct Contact </li></ul><ul><li>DON’T Tip the Refrigerator </li></ul><ul><li>IMMEDIATELY REMOVE GLOVES, SHOES OR ANY CLOTHING THAT BECOMES SATURATED </li></ul><ul><ul><li>If Skin Turns White, Seek Medical Attention </li></ul></ul>
    7. 7. Removing Frozen Stoppers <ul><li>Remove as Soon as Noticed </li></ul><ul><ul><li>Use Screwdriver to Pry the Stopper Out </li></ul></ul><ul><ul><li>Be Careful to NOT Damage the Neck Tube </li></ul></ul><ul><li>Keep the Stopper in Place and the Dust Cover Closed When Not in Use to Prevent a Frozen Stopper </li></ul>
    8. 8. Protect Your Semen Investment <ul><li>Because All Refrigerators WILL Eventually Fail, Systematically Monitor Nitrogen Levels </li></ul><ul><ul><li>To Check Nitrogen Level: </li></ul></ul><ul><ul><ul><li>Remove Neck Plug </li></ul></ul></ul><ul><ul><ul><li>Immerse a Centimeter-Marked Dipstick to Refer Bottom </li></ul></ul></ul><ul><ul><ul><li>Hold in Place for 8 to 10 Seconds </li></ul></ul></ul><ul><ul><ul><li>Remove and Re-cap Refer </li></ul></ul></ul><ul><ul><ul><li>Wave Dipstick in the Air For a Few Seconds </li></ul></ul></ul><ul><ul><ul><li>Read the Number Where the Whiteness Ends </li></ul></ul></ul>
    9. 9. User Responsibility Label
    10. 10. Check Liquid Nitrogen Regularly <ul><li>Determine Holding Time of Your Refrigerator </li></ul><ul><ul><li>If You’re Not Sure, Check the Table of Refrigerators Available From ABS </li></ul></ul>* Partial listing; Contact the Animal Products Department for a complete listing
    11. 11. Minimum Nitrogen Levels <ul><li>Check Level 4 Times per Fill </li></ul><ul><li>Refer to Chart for Minimum Levels </li></ul><ul><ul><li>Based Upon a 16-Week Refrigerator </li></ul></ul>If measured level is more than 2 cm below the Ok line, call your ABS Representative .
    12. 12. Refrigerator Fill Record <ul><li>Record Fill Levels on Form </li></ul><ul><li>Watch Data for Failures </li></ul>
    13. 13. Monitor Ampules <ul><li>Should Always Remain in Refrigerator </li></ul><ul><li>Kept on Rack With Blue ABS Tag on Top </li></ul><ul><li>Partially Filled With Blue Liquid </li></ul><ul><ul><li>Liquid Melts Before Semen is Damaged </li></ul></ul><ul><li>Stored Upside Down on the Rack </li></ul><ul><ul><li>Big End Toward the Top Tag </li></ul></ul>
    14. 14. How to Read Ampules <ul><li>Freeze Liquid in Bottom Ends </li></ul><ul><li>Invert Rack for Monitoring </li></ul><ul><li>If Canister or Refrigerator Warms Liquid Melts and Falls to Tip of Ampule </li></ul><ul><li>Read Ampules in Neck Tube in 10 Seconds or Less </li></ul>BAD GOOD
    15. 15. Semen Handling <ul><li>Quickly Transfer Straws Between Refrigerators </li></ul><ul><ul><li>Wear Gloves and Safety Glasses </li></ul></ul><ul><li>Use Two People to Transfer Racks Between Refrigerators </li></ul><ul><li>NEVER Expose Racks to Air Temperature for Longer Than 10 Seconds </li></ul>
    16. 16. Semen Inventory <ul><li>Record Semen Purchased for Each Sire and Location of Semen in Refrigerator and Canister </li></ul><ul><li>Mark Off Squares as You Remove Straws </li></ul><ul><li>The Number of Unmarked Squares Equals the Number of Straws of Semen Left </li></ul>
    17. 17. Summary <ul><li>Refrigerators Have Efficient Insulation System </li></ul><ul><li>Neck Tube Stopper Allows Gases to Escape </li></ul><ul><li>Keep Stoppers and Dust Cover in Place </li></ul><ul><li>Use Care in Removing Frozen Stoppers </li></ul><ul><li>Move the Refrigerator Carefully </li></ul><ul><li>Store the Refrigerator in a Clean and Dry Area </li></ul><ul><li>Always Wear Gloves and Safety Glasses </li></ul><ul><li>Regularly Check Refrigerator Performance </li></ul><ul><li>Use Monitor Ampules and Inventory Sheets </li></ul>
    18. 18. Question 1 <ul><li>A refrigerator’s neck tube stopper insulates liquid nitrogen and semen from outside air. </li></ul><ul><li>Why isn’t that stopper airtight? </li></ul>The stopper isn’t airtight in order to allow gases that are formed by the vaporizing nitrogen to escape. If gases were allowed to build up they could cause the tank to explode.
    19. 19. Question 2 <ul><li>What should you do if the stopper freezes in the neck tube? </li></ul>Use a screwdriver to carefully pry the stopper out of the neck tube. Be extremely careful to NOT puncture the neck tube.
    20. 20. Question 3 <ul><li>Where should you store your refrigerator? </li></ul>Always store your refrigerator in a clean, dry, well-ventilated area. The refrigerator should also be located where it is seen frequently each day in order to check for leaks or frozen stoppers.
    21. 21. Question 4 <ul><li>Refrigerators shouldn’t be set directly on concrete. </li></ul><ul><li>Why not? </li></ul><ul><li>Where should you set a refrigerator? </li></ul>A cement floor can cause corrosion and possible vacuum failure. The refrigerator should be kept on a wood floor or on boards or pallets placed over a cement floor.
    22. 22. Question 5 <ul><li>Liquid nitrogen is a very practical refrigerant. It’s nonflammable, non explosive and nontoxic. However, if you’re careless there are two ways you can be hurt. Name them. </li></ul><ul><li>What precautions should you take to avoid these problems? </li></ul>Freeze burns and suffocation Always wear gloves and safety glasses. Don’t splash or tip the refrigerator. NEVER store in a closed area.
    23. 23. Question 6 <ul><li>How frequently should you check the nitrogen level in you refrigerator with a dipstick? </li></ul><ul><li>Describe the procedure you would use. </li></ul>Check it four, equally spaced, times within the designated holding time. Remove the neck stopper. Immerse the dipstick to the bottom of the refrigerator for 8 -10 seconds. Then recap the refrigerator and wave the dipstick in the air for a few seconds. Read the nitrogen level (where the white frost ends).
    24. 24. Question 7 <ul><li>Monitor ampules are very sensitive to temperature changes that could affect semen quality. </li></ul><ul><li>How do you read them? </li></ul>If the liquid is frozen in the bottom of the ampule the tank is good. If the liquid has moved to the tip of the ampule the semen may not be good, even if the liquid is now re-frozen.
    25. 25. Question 8 <ul><li>As you look back over your “Refrigerator Fill and Liquid Level Record,” you see that “Check 2” line reads as follows: </li></ul><ul><li>What does this tell you about your refrigerator? </li></ul><ul><li>What if anything, should you do? </li></ul>Your refrigerator is slowly failing. Call your ABS Representative to come and pick up your properly cleaned tank to be sent in for testing.
    26. 26. Question 9 <ul><li>Describe a system that can be used to make locating a specific bull’s semen in the refrigerator easier. </li></ul><ul><li>How can you tell how many straws you have from a specific sire? </li></ul>Record semen purchased in an ABS Frozen Semen Inventory booklet. Because you cross off a square each time you use a straw of semen, you just need to count the number of unmarked squares left for that sire.