What areas of the school are the dirtiest?Is the surface dangerous to students just because it is dirty?How can we learn about bacteria in our environment?Can we adequately clean surfaces to protect ourselves?How does the environment at school affect the health of the students?
Dautie: I decided to investigate the amount of bacteria present on the IUPUI campus due to personalexperience. In the fall of I have a then 4strong interest Michelle: 2009, my very year old daughter, contracted the H1N1 virus.I She in germs and illness. guess since I Brittany: have become a mother toam Val: I I sick, requiring not want I work originally did hospitalizationbecame extremely chose this group because I moreand IV fluids. cautious of nearly worried with and take with germs. I our surrounding She was sick was 10 days about thought the project idea was it. I found it fascinating and taking those germ getting wantedscary that a virus interesting. I ill immunizedfind outcan and to additional, but necessary steps to try that we have all been against where the mosttous to family. sofamily from by home and my become I was inspired change and causegerms are my campus and getting prevent on severely ill.More than likely,teamspopular from and is interesting to how reliable thought it cleaner maturity the she a dedication touching or ill. I contracted would be when comingand decided to killing bacteria. thatreally into contact with a commonniche so its see howto findour surroundings I comes dirty my surface. Due to this, I are. If we can professional and that could work with a what kinds of began to wonder grow something germs are covering the surfaces we touch on a organized group. daily basis here on students concerns the campus. to scare them a little bit, then maybe they will also take the necessary steps to prevent bacteria growth - resulting in, potentially saving a life!
Course GoalsRichness- We chose our project based on what wethought would be interesting and what we had questionsabout. Throughout the final project we have used theinvestigative process. We are continuously askingquestions and are finding that there isn’t always onecorrect answer and many of our questions can’t beanswered.Recursion- Our project has required us to take notesand document our thoughts, questions, and details of theproject. We have used the inquiry process and examinedour project at the macro and micro level.Relations- The group project has required us to workand learn together. We each did our part and helped oneanother.Rigor- This project has helped us to better understandthat learning is on-going; that there are always morequestions to answer and more to learn.
Getting Ready to Gather GermsOur first day as a groupwe brainstormed allkinds of questionsabout germs, oursecond day weprepared to get ouranswers.Before we could collectour samples of germs inthe environment weneeded to prepare ouragar petri dishes.
Preparing the AGARMaterials: Microwave,microwaveable container,water, agar powder, plasticwrap, stir stick, 20 petridishes, permanent marker,tape.Procedure:1. Place agar powder into microwaveable container and add 2.5 cups or 600ml water, stir.2. Microwave 2 minutes, stir, and continue microwaving at 1 minute intervals and stirring until agar is completely dissolved.3. Cover and cool 3-5 minutes.
1. Lift lid off petri dish.2. Pour 1/8 to ¼ inch into dish.3. Immediately replace lid and tape.4. Allow to cool 30 min.5. Place in refrigerator until ready to use.
Plan of attack Investigate campus locations that have an increased amount of student activity, take samples. Supplies: 20 moist swabs, 20 Ziploc baggies, 10 Clorox disinfecting wipes, permanent marker. Locations: Bathroom Library stall Elevator Table work- interior Fountain top buttons station lock JagTagRevolving Vending Door Shoe condiment door machines Handle bottom table
Swab dirty surface with moist swab and place in labeled Ziploc bag. Clean area and allow to dry for 30 seconds.Swab area again with clean, moist swab and place in labeled Ziploc bag.Remove lid from labeled petri dish and swab in 4 quadrants, immediately replace lid and seal with tape.
Form Color Elevation What are we observing? We are observing the characteristics of the sample. Basically, what can we see on the macro and micro levels.Opacity Margin (Edge) Surface
Form - What is the basic shape of the colony?Elevation - What is the cross sectional shape of thecolony? Turn the Petri dish on end.Margin - What is the magnified shape of the edge of thecolony?Surface - How does the surface of the colony appear?Opacity - For example, transparent (clear), opaque, translucent(almost clear, like looking through frosted glass), iridescent.Chromogenesis (color) - For example, white, buff, red,purple, etc.There are 3 additional characteristics that can only be examinedin a supervised laboratory setting: consistency, emulsifiability,and odor.
School of Education Women’s 1stFloor Bathroom Door Lock InsideStall Dirty Sample Clean Sample
School of Education Water FountainNear Restroom on 1st Floor Dirty Sample Clean Sample
School of Education Elevator Buttons Dirty Sample Clean Sample
School of Social Work CommonEating Area Dirty Sample Clean Sample
University Library 4th Floor ComputerWork Station Dirty Sample Clean Sample
University Library Revolving Door(Handle and Glass) Dirty Sample Clean Sample
Jag Connection Condiment Area Dirty Sample Clean Sample
School of Social Work LargeVending Area Dirty Sample Clean Sample
School of Business StairwayDoor Handle Dirty Sample Clean Sample
The Bottom of a GroupMember’s Shoe Dirty Sample Clean Sample
Comparison of Colony Observation1601401201008060 Dirty40 Clean20 0
Effectiveness of Cleaning Agent Decrease in Growth After Cleaning 20% 80% Decreased Same or Increased
Decrease in Growth More Than 50% Decrease in Growth decreased growth by half 40% 60% less than 50% decrease in growth
Materials!!!1 ObservationSheet for each 4 Petri dishesgroup member 1 hand lens for each Characteristics group Sheet member
Activity Questions1. Look over the handout2. What do you observe in the petri dishes?3. Where were the samples taken? What kinds of surfaces?4. Are those areas places that you frequent?5. What could you do to the environment to make it safer? Yourself?6. What else do you want to know about GERMS?
Library Vending keyboard machines Fountain Cleaner Shoe was ineffective Table Revolving doors Elevator Jag Tag table Cleaner DoorBathroom was handle effective
Cleaner was Surfaces effective• Bathroom • Stainless• Elevator Steel• Table • Plastic• Revolving • Formica doors • Glass• Jag Tag table • Laminate• Door handle • Few crevices
Cleaner was ineffective We understand nowwhen label wasn’t that if the cleaner isfollowed. diluted or not used as intended it will not effectively clean theFountain: the surface was surface.wet with many crevices.Library keyboard: Manycrevices and I was afraid toget the cleaner in theelectronics.Vending machines: So Now we wonder, whatmany crevices that couldn’t bereached with a wipe. if we used a cleaner that was an aerosol orShoe: So many crevices that scrubbed the porouscouldn’t be reached with awipe and so dirty. dirty surfaces?
Cleaning ConclusionsUse cleaning products accordingto label for best results.Avoid areas that are visiblydirty and difficult to cleanthoroughly.
Interview with Dr. AndersonIUPUI Biology Department What We Found Out!We had hoped to answer some of our questions about thetypes of bacteria that are growing. Unfortunately the biologydept. does not have the resources to conclude what we havegrowing in our petri dishes.Dr. Anderson was able to explain: -we were not growing any viruses, only bacteria and fungi. -some are always harmful to your body- like strep and meningitis. -most bacteria are not harmful unless we are exposed to them in large amounts.
How It Affects You!Many of the bacteria and viruses can lead to otherillnesses like food poisoning, pneumonia, bronchitis, andmany types of infections, including fungal infections.Taking a daily vitamin can boost your immune systemand help you stay healthy!Using Clorox disinfecting wipes is just one way to reducecontact with bad germs! Can you think of others? Common Infections Clorox Wipes Kill: Found on Campus: -Cold/flu - Staph infection -Strep throat - Strep throat -Meningitis - Cold/flu virus -Mono - Salmonella - E. Coli - Meningitis
Campus/Illness Facts Schools are an excellent breeding ground for bacteria/diseases.Lack of sleep during “crunch time” can bring your immune system down. The two most Common Bacteria found on campuses are meningitis, and strep throat. Meningitis Symptoms include fever, nausea, vomiting, headache, sensitivity to light, nasal congestion and a stiff neck. Meningitis is often confused with a less serious illness such as a cold or the flu. Strep Throat Symptoms include difficulty swallowing, red or white spots in the throat, swollen or sore glands, fever, rash and digestive pain or discomfort.
What is the # 1 thing you can do to prevent the spread of these germs? Hand Washing:It’s easy to do and its one of the most effective ways to prevent the spread of many types of illnesses.Clean hands can stop germs from spreading from one person to another.
Did you know?Most people do not wash their hands correctlyor long enough. 1. UseStart NOW!! plenty of 2. Use 3. Dry hands anti-bacterial running thoroughly. soap water20 seconds should be the amount of timefor hand washing! Next time you wash yourhands count and see if you pass the test!Tip: Sing the ABC song or Happy Birthday for theduration of hand washing!
Fact from CDC If you start washing yourhands correctly for20 seconds or more this could save up to 1 million lives per year! www.cdc.gov
When should you wash your hands?preparing foodBefore eatingAfter using the bathroomAfter changing diapersBefore and after caring for someone who is sickAfter blowing your nose, coughing, or sneezingAfter touching an animalAfter touching garbageBefore and after treating a cut or wound
More….. Preventative measures Do not Get plenty share Use hand Stay of rest eating sanitizer utensils. (containing away (8 hrs. or more that from more per 6% alcohol) sick night) if soap and people. water is not available. Cough and sneeze into your Use elbow, not disinfectants Get Vaccinated your or cleaners on (meningitis hands backpacks, vaccine bottom of recommended shoes, writing utensils, for college laptops, and students) eating areas.
Resources:Thiel, T. (1999). Streaking microbial cultures on agar plates.Science in the Real World: Microbes in Action, Retrieved fromhttp://www.umsl.edu/~microbes/techniques.htmlThiel, T. (1999). Tips for pouring and storing agar plates. Science inthe Real World: Microbes in Action, Retrieved fromhttp://www.umsl.edu/~microbes/techniques.htmlLiu, S. (2005). Interpreting plates. Science Buddies, Retrieved fromhttp://www.sciencebuddies.org/science-fair-projects/project_ideas/MicroBio_Interpreting_Plates.shtml Clorox.Clorox disinfecting wipes. (2011, April 27). Retrieved fromhttp://www.clorox.com/products/clorox-disinfecting-wipes/faq/Wash your hands. (2011, April 27). Retrieved fromhttp://www.cdc.gov/Features/HandWashing/Biology & nature. (2011, April 27). Retrieved fromhttp://www.sciencebobstore.com/