Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.
How to Prepare Permanent Slides
                              Mark Goodman

This procedure describes a method for preparin...
1. The Meltmount is solid at room temperature and needs to be
   melted on the hot plate prior to use at about 60-70 °C (u...
Upcoming SlideShare
Loading in …5
×

Permanent Slides

13,990 views

Published on

A permanent slide prepartion method is presented

  • Login to see the comments

Permanent Slides

  1. 1. How to Prepare Permanent Slides Mark Goodman This procedure describes a method for preparing permanent microscope slides. I use this method when preparing fiber slides which are retained for future reference or instruction. Materials: • Cargille Meltmount - Cargille Laboratories ND=1.662 – comes in a jar or as a stick (QuickStick). • • Hot plate - should be able to reach at least 100 °C. Some have a heater and a stirrer. A heater is all that is required. • Slides • Slide covers – circular recommended • Glass rod or implement for Meltmount transfer to slide A hot plate is a small electric stove used in laboratory settings to heat glassware. They are generally expensive but can be purchase on E-bay for about $20 - $30. The Meltmount can be purchased in a stick or in a glass container. The Meltmount should be transferred to a heat resistant container such as a Pyrex beaker after melting.
  2. 2. 1. The Meltmount is solid at room temperature and needs to be melted on the hot plate prior to use at about 60-70 °C (usually the lowest setting). 2. Select the fiber sample to be mounted and place on a clean slide where desired. 3. Pick up a small amount of the Meltmount I use a glass rod and while liquid place it onto a clean slide cover. I put enough on to get a thin layer on most of the slide cover. Too much Meltmount may produce a messy slide with a lot of excess Meltmount to be cleaned off later. Too little Meltmount may not adequately handle large particles, leaving a tilted slide cover. 4. Place the slide cover on top of the fiber sample on the slide. 5. Put a slide on the hot plate with about ¼ of the slide off the hot plate's surface. 6. Once the Meltmount is completely liquefied the slide should be removed quickly and a clean glass rod or other implement should be used to gently press down the slide cover taking care not to crack the glass. This is done to remove any air bubbles. 7. One the slide has cooled clean off any excess Meltmount with a razor blade. 8. Label the slide.

×