Agenda   October 31, 20121. Take-up Microscope worksheet2. Field of View (FOV)3. Calculate actual size of object4. Get to ...
Field of View (FOV) To MEASURE FOV,  1. Set the microscope to a low, medium, or high magnification by rotating     the no...
to CALCULATE Filed of View   If you know the diameter of the FOV for low power lens, FOV of   the higher power lenses can ...
Size of the objectCalculate actual size of the object  Size of the object:                   _________Field of View (FOV)_...
Size of the object:                   _________Field of View (FOV)_______________  Actual size =                   # of ti...
Microscope Lab
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Ms. He's Microscope Lab

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Ms. He's Microscope Lab

  1. 1. Agenda October 31, 20121. Take-up Microscope worksheet2. Field of View (FOV)3. Calculate actual size of object4. Get to know your microscope5. Wet mount Lab6. Homework: study for Quiz
  2. 2. Field of View (FOV) To MEASURE FOV, 1. Set the microscope to a low, medium, or high magnification by rotating the nosepiece 2. Place a clear plastic ruler on the stage 3. Focus the ruler until you see the field of view 4. Measure the diameter of the filed of view in millimeters
  3. 3. to CALCULATE Filed of View If you know the diameter of the FOV for low power lens, FOV of the higher power lenses can be calculated,Ratio of the Magnification: magnification of lower power lenshigher-power FOV = lower power FOV x magnification of higher power lens Practice Calculation • If the 10x low power FOV is 10mm, what is the 100x high power FOV? 10mm x (10/100) = 1 mm
  4. 4. Size of the objectCalculate actual size of the object Size of the object: _________Field of View (FOV)_______________ Actual size = # of times a cells fits across the field of view * The actual size is approximate
  5. 5. Size of the object: _________Field of View (FOV)_______________ Actual size = # of times a cells fits across the field of viewFit across FOV approximately 4 times Actual size = 4 mm 4 = 1mm
  6. 6. Microscope Lab

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