Liuping

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Liuping

  1. 1. Effects of solution extracted from vermicompost Reporter :Liuping Major :plant nutrient
  2. 2. Introduction Vermicompost is the product of organic solid waste , digested by earthworms, with the advantage of high porosity, fine ventilation and drainage performance ,as well as strong water retection capacity . Aim to provide a technical guidance for selection organic , environmental and economical edible vegetable seedlings substrate prescription and a theoretical basis on resource utilization .
  3. 3. Material and methods Maize seedlings /capsicum seedlings/Brassica pekinensis Methods : hydroponic culture /pot incubation test/ foliage spraying
  4. 4. Hoagland Arnon Nutrient solution formula Microelements mg/L H 3 BO 3 2.86 CuSO 4 · 5H 2 O 1.81 ZnSO 4 · 7H 2 O 0.22 MnCl 2 · 4H 2 O 0.08 H 2 MoO 4 · 4H 2 O 0.09 Macroelements g/L Ca(NO 3 ) 2 · 4H 2 O 1.18 KNO 3 0.51 MgSO 4 · 7H 2 O 0.49 KH 2 PO 4 0.14 Fe EDTA 0.005
  5. 5. Extraction solution Vermicompost : distilled water=1:1(V:V) Stir heavily per 8hours (4times in total) Centrifugal to separate Store in a 4 ℃ refrigerator
  6. 6. Nutrient solution Extraction Treatment Ⅰ Treatment Ⅳ Treatment Ⅲ Treatment Ⅱ
  7. 7. Chlorophyll content Nutrient (N) Nutrient3: Extraction1 Nutrient1: Etraction1 Extraction(E)
  8. 8. Fresh Root-Shoot Ratio E E1: N1 E1: N3 N
  9. 9. Dry Root-shoot Ratio E E1: N1 E1: N3 N
  10. 10. Root analysis system Type: WinRHTZ 2005a Length(cm) ProjArea(cm2) SurfArea(cm2) AvgDiam(mm) E 563.21 19.90 62.50 0.35 E1:N1 556.80 21.54 67.66 0.39 E1:N3 635.10 24.46 76.86 0.38 N 582.67 20.00 62.85 0.36
  11. 11. LenPerVol(cm/m3) RootVolume(cm3) Tips Forks Crossings E 563.21 0.56 665.67 1512.00 295.33 E1:N1 556.80 0.65 704.00 1695.00 327.33 E1:N3 635.10 0.75 928.00 2121.67 408.67 N 582.67 0.54 715.33 1424.00 285.00
  12. 12. Root Length Parameter
  13. 13. Root Surface Area Parameter
  14. 14. Planned work Capsicum seedlings sampling and cultivating Set up more than 7 treatments in proportion of N,N8:E1,N4:E1,N1:E1,N1:E4,N1:E8 and E, with 3 replications. Foliage spraying Detect the elements of extracted solution Micromorphological analysis
  15. 15. Thin section investigation Ultrathin section Master Ultramicrotomy and be able to use Ultramicrotome Preparation of thin section Separation of microorganism and analysis of microbial activity Finally determine causes for vermicompost’s promotive effect on root

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