Airlift

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this ppt is abt one of the types of fermenters used for bioprocessing... hope its useful!!

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Airlift

  1. 1. THE AIR LIFT FERMENTERS
  2. 2. INTRODUCTION… <ul><li>Type of fermenters that are impeller-free systems. </li></ul><ul><li>Employ forced/ pressurised air to circulate cells and nutrient medium. </li></ul><ul><li>Can be used to culture cells that highly shear-sensitive. </li></ul>
  3. 3. STRUCTURE / DESIGN… <ul><li>Entire reactor is divided into 2 halves by a Draft tube: </li></ul><ul><li>inner gassed region ( Riser) </li></ul><ul><li>outer ungassed region ( Down comer) </li></ul><ul><li>Riser has gas injection connected- air moves upwards . </li></ul><ul><li>Down comer region has degassed media and cells. </li></ul><ul><li>Mean density gradient between riser and downcomer regions causes continuous circulation. </li></ul>
  4. 5. PARTS OF ALR… <ul><li>RISER : Connected gas injection- upward air flow. </li></ul><ul><li>DOWNCOMER : degassed media+cells. </li></ul><ul><li>BASE : Connected to Perforated nozzle bank/ plate/ Sparger to pump pressurized air. </li></ul><ul><li>HEAD SPACE : Gas release region, flocculation, foam accumulation etc. </li></ul><ul><li>GAS SEPARATOR : </li></ul><ul><li>Facilitates gas/liquid recirculation </li></ul><ul><li>maximizes gas residence time </li></ul><ul><li>reduces gas friction in downcomer. </li></ul>
  5. 6. Types of Gas separators in Internal loop ALRS
  6. 7. Types of Gas separators in External loop ALRs
  7. 8. TYPES OF ALRs….
  8. 9. Internal Loop reactor External Loop reactor
  9. 10. ADVANTAGES OF ALRs… <ul><li>Sparger has 2 functions: Aeration as well as Agitation. </li></ul><ul><li>Hence, homogenous mixing/ distribution of gas/ oxygen. </li></ul><ul><li>Homogenous distribution of nutrients and shear force. </li></ul><ul><li>No focal points of energy/ heat dissipation- no agitator or stirrer. </li></ul><ul><li>Key factors: </li></ul><ul><li>Regulation of air bubble size </li></ul><ul><li>Bubble residence time </li></ul><ul><li>Liquid circulation and Gas injection. </li></ul>
  10. 11. MODIFICATIONS OF ALRs…
  11. 12. USES…. <ul><li>Mammalian cell cultures. </li></ul><ul><li>Waste water treatment </li></ul><ul><li>Biological processes involving biocatalysts as solids </li></ul><ul><li>To produce biopharma proteins etc from fragile cells. </li></ul>
  12. 13. THANK YOU…..

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