this ppt describes about the morphological analysis of micro algae and cyanobacteria and also it gives idea about the preparation of permanent slide .

1. MORPHOLOGICAL CHERACTERIZATION OF
MICRO-ALGAE AND CYNOBACTERIA
PRESENTING BY
Sunirmal Das
B.Tech ,Final year
Bio engineering 1
Under the guidance of
Dr. Tridib Kumar Bhowmick
Co-guide
Dr. Kalyan Gayen

2. Micro-algae and Cynobacteria
2
• Found in freshwater and marine systems living in both the
water column and sediment.
• They are unicellular species which exist individually, or in
chains or groups.
• Microalgae, capable of performing photosynthesis, are
important for life on earth.
• Bio diversity of microalgae is enormous estimated that
about 200,000-800,000 species in many different genera
exist of which about 50,000 species are described.
• Algae can be use as Fuel source
(biodiesel,bioethanol,biobutanol), food supplement,
Stabilizing agent, Fertilizer.

3. 3
• PERMANENT SLIDE PREPARATION OF MICRO-ALGAE AND
CYNOBACTERIA.
• MORPHOLOGICAL CHERACTERIZATION OF MICRO-ALGAE AND
CYNOBACTERIA.

4. Permanent slide preparation
4
What...??
A permanent slide is a slide can be fixed and locked into place. While temporary slides
usually have a top and a bottom with the object secured in-between, permanent slides
have a top and bottom that are glued or sealed together for longer time.
Why…??
• Permanent slides have the advantage of being vacuum-sealed within frame to
prevent movement or leakage.
• Ideal for transport or repeated presentational viewings.
• With permanent slides we can preserve the specimen for longer than temporary
slide.

5. Permanent slide preparation
How…??
Materials & equipment required
1. Fresh water.
2. Sample of algae.
3. Formaldehyde(40%).
4. Filter paper.
5. Slide.
6. Cover slip.
7. Oven.
8. Microscope.
9. Glycerine jelly.
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Referance: Text book of algae by O.P.Sharma , passage no 6.5.1, laboratory culture and staining
page no:71

6. Permanent slide preparation
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Procedure
1. Wash the slide and cover slip properly so that there is no particles .
2. Put a drop of fresh water on slide.
3. Wash the algae with PBS 10m for 2 times.
4. Place the algae on the slide .
5. Add a drop of formalin (40%).
6. Use filter paper to drain away excess liquid.
7. Put some glycerine on algae.
8. Keep the slide on oven for 2-3 minutes at 60°c.
9. Put washed cover slip on it.
10. Again keep the slide in oven for 2-3 minutes .
11. Remove the excess glecerine .
12. Seal it properly with glu or nail polish .
Referance: Text book of algae by O.P.Sharma , passage no 6.5.1, laboratory culture and staining
page no:71

7. Morphology Characterization
7
What…??
Morphological characterization is identification of species and structural
analysis of it .
Task is to identify the species from different classes of micro-algae and
cynobacteria.
How…??
Stage 1
Preparation of good permanent slide.
Stage 2
Take good sample photos using microscope & camera.

8. Morphology Characterization
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Stage 3
Identification and morphology characterization.

9. 9
Month Week Task Status
Dec 1st week Mid term
2nd week Literature morphology
3rd week Literature morphology
4th week Literature morphology
Jan 1st week Literature morphology
2nd week Basic operations of lab
3rd week Basic operations of lab
4th week Basic operations of lab
Feb 1st week Preparation of slide &
microscopic photos
2nd week Preparation of slide &
microscopic photos
3rd week Preparation of slide &
microscopic photos
4th week Preparation of slide &
microscopic photos
Mar 1st week Morphological study
2nd week Morphological study
3rd week Morphological study
4th week Mid term
Apr 1st week Final report preparation
2nd week Final report preparation
3rd week Final presentation

10. Thank you
10