1.
Cross Sectional Study Prof. Wei-Qing Chen MD PhD Department of Biostatistics and Epidemiology School of Public Health 87332199 [email_address]

2.
Learning/Performance Objectives <ul><li>To develop an understanding of: </li></ul><ul><ul><li>What cross-sectional study is </li></ul></ul><ul><ul><li>The use of this study </li></ul></ul><ul><ul><li>The basic methodology of it </li></ul></ul><ul><ul><li>Advantage and disadvantage of is </li></ul></ul>

3.
Definition <ul><li>Cross-sectional studies are studies of prevalence. Proportion with an attribute or disease / Number of subjects = Prevalence. </li></ul><ul><ul><li>a type of observational or descriptive study </li></ul></ul><ul><ul><li>the research has no control over the exposure of interest (e.q. diet). </li></ul></ul><ul><li>3 important questions to consider: </li></ul><ul><ul><li>Definition of Case </li></ul></ul><ul><ul><li>Definition of the Population </li></ul></ul><ul><ul><li>Are cases and non-cases from an unbiased sample of the population? </li></ul></ul>

5.
Definition <ul><li>“ Snapshot Studies” (Paffenbarger, 1988) </li></ul><ul><li>Observations at a single hypothetical point in time </li></ul><ul><li>Each subject assessed once at point in time. </li></ul><ul><li>Point Prevalence Studies </li></ul>

6.
Definition <ul><li>also called a Prevalence survey </li></ul><ul><li>A study that is quick and inexpensive to complete. </li></ul><ul><li>Designed to determine “ what is happening ? right now” </li></ul>

7.
Basic features <ul><li>“ Snapshot” of a population, a “still life” </li></ul><ul><li>Assesses both the exposure and outcome simultaneously, at a single point in time </li></ul><ul><li>Calculates prevalence, but not incidence </li></ul><ul><li>A study that is quick and inexpensive to complete. </li></ul><ul><li>The first step in testing associations </li></ul>

8.
Uses <ul><li>Prevalence survey: The studies are commonly used to describe the burden of disease in the community and its distribution. </li></ul><ul><li>D escribe population characteristics: They are also commonly used to describe population characteristics, often in terms of person (who?) and place (where?) </li></ul><ul><ul><li>.e.q. </li></ul></ul><ul><ul><li>The British National Diet and Nutrition Survey or Nutrition and Health Survey in Taiwan </li></ul></ul><ul><ul><li>To describe various age groups in the population in terms of food and nutrient intake and range of other personal and lifestyle characteristics. </li></ul></ul>

9.
Uses <ul><li>Prevalence used in planning </li></ul><ul><ul><li>Individual: Pre-treament probability for Rx and Dx </li></ul></ul><ul><ul><li>Population: Health care services </li></ul></ul><ul><li>Examine associations among variables </li></ul><ul><li>Hypothesis generating for causal links </li></ul><ul><li>Prediction rule eg, Ottawa ankle rule – XR if 3 factors present </li></ul>

10.
<ul><li>KAP (knowledges, attitudes, and practices ) study: </li></ul><ul><ul><li>KAP studies are purely descriptive and help to build up a better understanding of the behavior of the population, without necessarily relating this to any disease or health outcome. </li></ul></ul><ul><li>Management tool : </li></ul><ul><ul><li>health service managers and planners may make use of cross-sectional survey to assess utilization and effectiveness of service. </li></ul></ul>Uses

11.
Uses <ul><li>Identify and describe a problem </li></ul><ul><li>Collect information for planning e.g. surveys of immunisation, antenatal care, coverage </li></ul><ul><li>Evaluate utilisation rates of services </li></ul><ul><li>Monitoring health status of a community by regular repeated surveys </li></ul>

12.
Uses <ul><li>Hypothesis generating for causal links </li></ul><ul><ul><li>Method of Difference : If frequency of a disease is markedly different between two groups then it is likely to be caused by a particular factor that differs between them. </li></ul></ul><ul><ul><li>Method of Agreement : If a factor commonly occurs in which a disease occurs with high frequency then the factor is very likely associated with the disease. </li></ul></ul><ul><ul><li>Concomitant variation : Frequency of a factor varies in proportion to frequency of disease. </li></ul></ul>

13.
Measure: Prevalence <ul><li>Measure exposure and outcome variables at one point in time. </li></ul><ul><li>Main outcome measure is prevalence </li></ul><ul><li>P = Number of people with disease x at time t </li></ul><ul><li> Number of people at risk for disease x at time t </li></ul><ul><li>Prevalence=k x Incidence x Duration </li></ul>

14.
Measure: Prevalence Example: RQ: What is the prevalence of chronic pain after hernia surgery? Exposure of interest : Hernia surgery Outcome of interest : Chronic pain (lasting for more than 3 months) Methods: questionnaire survey Sample: All patients who had a hernia procedure between 1995-1997 n=350 Results: Period prevalence chronic pain = 30% (CI 95% 24 - 36%) Point prevalence chronic pain = 25% (on day of survey)

15.
Prevalence vs. Incidence <ul><li>Prevalence </li></ul><ul><ul><li>The total number of cases at a point in time </li></ul></ul><ul><ul><li>Includes both new and old cases </li></ul></ul><ul><li>Incidence </li></ul><ul><ul><li>The number of new cases over time </li></ul></ul>

16.
Interpretation <ul><li>Measures prevalence – if incidence is our real interest, prevalence is often not a good surrogate measure </li></ul><ul><li>Studies only “survivors” and “stayers” </li></ul><ul><li>May be difficult to determine whether a “cause” came before an “effect” (exception: genetic factors) </li></ul>

17.
Design of cross-sectional survey

18.
Basic Design <ul><li>Cross-sectional study involves no follow-up of individuals, so are often grouped together </li></ul><ul><li>In addition, this study depends on a full accounting or random cross-section of the population </li></ul><ul><li>This design is capable of measuring prevalences and open population incidence rates: </li></ul>Prevalence or rate, group k Compare prevalence or rates Random sample of population divided into exposure groups Prevalence or rate, group 1 Prevalence or rate, group 2 : :

19.
Study Design Exposure (Risk Factor) Disease (Outcome) + + _ _

20.
Things to consider when designing a cross-sectional study (survey) <ul><li>What is your research question? </li></ul><ul><li>Is the design appropriate for your study? </li></ul><ul><li>Who are you going to study? </li></ul><ul><li>How are you going to obtain your sample? </li></ul><ul><ul><li>Everyone who is eligible should have an equal chance of being invited to take part </li></ul></ul><ul><li>Is there a risk of ‘selection bias’? </li></ul><ul><ul><li>E.g. taking people attending a specialist clinic; might not be ‘representative’ of all patients with that condition </li></ul></ul><ul><ul><li>Selection bias is a threat </li></ul></ul><ul><li>How you will collect your exposure/outcome data </li></ul><ul><li>Think about analysis (proportion %, denominator) </li></ul>

21.
<ul><li>The problem to be studied must be clearly described and a thorough literature review undertaken before starting the data collection. </li></ul><ul><li>Specific objectives need to be formulated. </li></ul><ul><li>The information has to be collected and data collection techniques need to be decided. </li></ul><ul><li>Sampling is a particularly important issue to ensure that the objectives can be met in the most efficient way. </li></ul>Things to consider when designing a cross-sectional study (survey)

22.
<ul><li>In Cross-sectional studies think of: </li></ul><ul><ul><li>Sampling Procedures. </li></ul></ul><ul><ul><li>Clear definition of Target Population. </li></ul></ul><ul><ul><li>Clear definition of outcome. </li></ul></ul><ul><ul><li>Clear definition of risk factors. </li></ul></ul><ul><ul><li>Remember Confounders. </li></ul></ul>Things to consider when designing a cross-sectional study (survey)

23.
<ul><li>Fieldwork needs planning: </li></ul><ul><ul><li>Who is available to collect the data ? </li></ul></ul><ul><ul><li>Do they need training ? </li></ul></ul><ul><ul><li>If more than one is to collect the data then it is necessary to assess between-observer variation. </li></ul></ul><ul><li>The collection, coding and entry of data need planning. </li></ul><ul><li>A pilot study is essential to test the proposed methods and make any alternations as necessary. </li></ul><ul><li>* The steps are summarized in Fig 13.5* </li></ul>

26.
Sampling <ul><li>Sampling </li></ul><ul><ul><li>A sample is a subset of the population </li></ul></ul><ul><ul><li>Can be random or non-random; can be representative or non-representative </li></ul></ul><ul><ul><li>Different types of sampling </li></ul></ul><ul><ul><li>This is major challenge when doing cross-sectional studies </li></ul></ul>

27.
Sample size estimation <ul><ul><li>Purpose: adequate power of test </li></ul></ul><ul><ul><li>basic formula and necessary components </li></ul></ul><ul><ul><ul><li>alpha (one or two-sided) and beta error </li></ul></ul></ul><ul><ul><ul><ul><li>usually alpha = 0.05, beta = 0.2 </li></ul></ul></ul></ul><ul><ul><ul><ul><li>then power = 1-beta = 0.8 </li></ul></ul></ul></ul><ul><ul><ul><li>effective size: mean, difference, ratio, ... </li></ul></ul></ul><ul><ul><ul><li>standard deviation </li></ul></ul></ul><ul><ul><ul><ul><li>from prior information or other related source </li></ul></ul></ul></ul><ul><ul><li>formula/tables/softwares </li></ul></ul>

28.
Types of sample <ul><li>1) The Random Sample </li></ul><ul><li>2) Systematic Sampling </li></ul><ul><li>3) Stratified Sampling </li></ul><ul><li>4) Cluster and Multistage Sampling </li></ul><ul><li>5) Convenience Sampling </li></ul>

29.
1-1) The Random Sample <ul><li>A Random Sample is the most representative sample of all population. = Golden Standard= Every member of population must have an equal chance of being picked for the sample. </li></ul>

30.
1-2) Systematic Sampling <ul><li>800 women are all routinely tested for genital chlamydia as part of a general health check in the 5 women’s clinics for 12 months. You decide that a sample of 500 women will be big enough. Suppose you find that there are 8000 such patients' records in total, and you decide to take every sixteenth record, which will give you 500 records in total. This is a systematic sample. </li></ul><ul><li>Provided that a sample of 500 is big enough to detect a condition which might occur infrequently, the sample should be reasonably representative--but representative of the woman attending your five clinics, and not necessarily representative of the entire 16+ female population of the USA. </li></ul><ul><li>Notice that taking a systemic sample need a sampling frame. </li></ul>

31.
I-3) Stratified Sampling <ul><li>Su ppose you have a particular interest in the occurrence of genital chlamydia in women from some ethnic minority, who you know account for only 10% of your population. </li></ul><ul><li>To ensure that these women are represented in adequate numbers in yo ur sample (around 10% of the sample), you could separate out the ethnic minority women's records first and then take every sixteenth record from both groups, until you've got 50 from the minority group and 450 fro m the rest. </li></ul><ul><li>This process is known as stratified sampling. </li></ul><ul><li>Y ou need a sam p ling frame for this procedure . </li></ul>

32.
I-4) Cluster and Multistage Sampling <ul><li>You could expand your population to include all of the women ’s clinics in your health authority; let's say there are 30 clinics. </li></ul><ul><li>You could take a random sample of five clinics from these 30, and your subjects would then be all of the women in these selected clinics . </li></ul><ul><li>This approach is known as cluster sam p Iing. </li></ul><ul><li>An alternative approach would be to take a random selection from the 30 clinics and then take a random selection of patients in those clinics. This is m u l tistage sa mpling . A sampling frame is not necessary for this me thod of sam p ling . </li></ul>

33.
I-5) Convenience Sampling <ul><li>O ne approach to the sampling problem is to take as your sample those subjects who are conveniently to hand: perhaps the last 100 patients to attend a certain clinic, or all of those patients who attended during the past 12 months. </li></ul><ul><li>The attraction of co n venience sampling is that it is just that, convenien t. </li></ul><ul><li>One obvious problem with this approach is that it is questionable what population such a sample is representative of. </li></ul><ul><li>In truth, it is extremely difficult to take anything like a true random sample in the healthcare arena. </li></ul><ul><li>The practical and ethical dif fi culties associated with such a process are simply too great. </li></ul>

34.
Data Collection <ul><li>Ordinary data ： medical records and reporting cards or tables </li></ul><ul><ul><li>Advantage ： </li></ul></ul><ul><ul><ul><li>Easy obtaining ； easily making dynamic analysis and secular trend ； easily obtain lots of valuable information in short time. </li></ul></ul></ul><ul><ul><li>Disadvantage ： </li></ul></ul><ul><ul><ul><li>Poor in the whole ； criteria of diagnosis being different at different period ； poor in reliability </li></ul></ul></ul>

35.
<ul><li>Temporarily data ： To reach a certain aim, a special survey will be conducted for collecting data based on study design and the aim of survey. </li></ul>Data Collection

36.
<ul><li>face to face interview </li></ul><ul><li>mail questionnaire </li></ul><ul><li>telephone interview </li></ul><ul><li>Self-administrated questionnaire </li></ul><ul><li>Medical examination </li></ul><ul><li>Laboratory test </li></ul>Methods for collecting data

37.
<ul><li>To sure what data shall be obtained </li></ul><ul><li>To sure which index will be used </li></ul><ul><li>Methods for collecting data </li></ul><ul><li>Criteria of disease diagnosis </li></ul><ul><li>Definition of variables </li></ul><ul><li>Training investigators </li></ul>Issues in collecting data

38.
Dietary assessment in cross-sectional studies <ul><li>Some characteristics of dietary assessment methods for cross-sectional studies </li></ul><ul><ul><li>Measures an individual’s intake at one point in time. </li></ul></ul><ul><ul><li>Does not require long-term follow up or repeat measures </li></ul></ul><ul><ul><li>Valid </li></ul></ul><ul><ul><li>Reproducible </li></ul></ul><ul><ul><li>Suitable </li></ul></ul><ul><ul><li>Cost within study budget </li></ul></ul>

39.
Dietary method application <ul><li>Food records using household measures have been used in cross-sectional studies. </li></ul><ul><li>The recall method attempts to quantify diet over a defined period in the past usually 24 hours. </li></ul><ul><li>The most commonly used dietary assessment method which attempts to measure usual intake is the food frequency questionnaire (FFQ). </li></ul>

42.
Analysis <ul><li>Before starting any formal analysis, the data should be checked for any errors and outlines. </li></ul><ul><ul><li>Obvious error must be corrected. </li></ul></ul><ul><ul><li>The records of outliners should be examined excluded </li></ul></ul><ul><ul><li>Checking normality of data distribution. </li></ul></ul><ul><ul><ul><li>e.q. using the Kolmogorov-Smirnov Goodness of Fit Test. </li></ul></ul></ul>

43.
Analysis <ul><li>Descriptive analyses </li></ul><ul><li>Analysis of differences </li></ul><ul><li>Analysis of association / relationship </li></ul><ul><li>Multivariable analysis </li></ul>

44.
Analysis <ul><li>or “PREVALENCE </li></ul><ul><li>STUDY” </li></ul><ul><li>Hallmark: </li></ul><ul><li>Risk factors (exposures) and disease outcome are ascertained at a single point in time in a cross-sectional sample of subjects. </li></ul>AKA: “SURVEY”

45.
<ul><li>Standard descriptive statistics can then be used: mean, median, quartiles, and mode; measure of dispersion or variability such as : standard deviation; measure precision such as: standard error, and confidence intervals. </li></ul><ul><li>Mean can be compared using t-tests or analysis of variance (ANOVA). </li></ul><ul><li>More complex multivariate analysis can be carried out such as multiple and logistic regression. </li></ul>Analysis

46.
Analysis Grape Tomato Prevalence ratio = 52% / 19% = 2.6 (+) (–) DZ = Rash 183 43 95 88 8 35 (19%) (52%)

47.
Analysis <ul><li>Instead of looking at a ratio of prevalences, we can also look at a ratio of odds. </li></ul><ul><li>Odds are not intuitively appealing: they are the likelihood of an event occurring divided by the likelihood of the event not occurring. </li></ul>

48.
Analysis Grape Tomato (+) - DZ = Rash 95/183 PR= ------- =2.6 8/43 Odds of grape work in rash pts: (95/ 103 ) / (8/ 103 ) = 95 / 8 =11.9 Odds of grape work in healthy: (88/ 123 ) / (35/ 123 ) = 88 / 35 =2.5 183 43 103 123 <ul><li>35 </li></ul><ul><li>8 </li></ul><ul><li>88 </li></ul><ul><li>95 </li></ul>

49.
Analysis Grape Tomato (+) - DZ = Rash 95/183 PR= ------- =2.6 8/43 Odds of grape work in rash pts: 95 / 8 =11.9 Odds of grape work in healthy: 88 / 35 =2.5 Odds ratio=( 95 / 8 )/( 88 / 35 )=11.9/2.5=4.7 183 43 <ul><li>35 </li></ul><ul><li>8 </li></ul><ul><li>88 </li></ul><ul><li>95 </li></ul>

50.
Bias <ul><li>Selection Bias (eg, NSSP study) </li></ul><ul><ul><li>Is study population representative of target population? </li></ul></ul><ul><ul><li>Is there systematic increase or decrease of RF? </li></ul></ul><ul><li>Measurement Bias </li></ul><ul><li>Outcome </li></ul><ul><li>Misclassified (dead, misdiagnosed, undiagnosed) </li></ul><ul><li>Length-biased sampling </li></ul><ul><ul><li>Cases overrepresented if illness has long duration and are underrepresented if short duration.(Prev = k x I x duration) </li></ul></ul><ul><li>Risk Factor </li></ul><ul><li>Recall bias </li></ul><ul><li>Prevalence-incidence bias </li></ul><ul><ul><li>RF affects disease duration not incidence eg, HLA-A2 </li></ul></ul>

51.
Bias <ul><li>The selection bias classic for cross-sectional studies is “the healthy worker effect.” I.e., only “healthy workers” are available for study, distorting your findings. </li></ul><ul><li>Example: Low asthma rates in animal handlers (because persons contracting asthma quit and are not available for study). </li></ul>

52.
Advantages <ul><li>Quick, cheap </li></ul><ul><li>Easy to obtain prevalence </li></ul><ul><ul><li>Outcome </li></ul></ul><ul><ul><li>Exposure </li></ul></ul><ul><li>Can adapt design </li></ul><ul><ul><li>Case-control study </li></ul></ul><ul><ul><li>Prospective cohort study </li></ul></ul>

53.
Disadvantages <ul><li>Prone to selection bias </li></ul><ul><li>Recall bias </li></ul><ul><li>Cannot measure disease onset </li></ul><ul><li>Problem of temporality (not a problem if exposure is constant) </li></ul><ul><li>Not suitable for rare disease </li></ul>