Multi-functional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus . Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types. Anti-TGF-ß Antibody (STJ95997): http://www.stjohnslabs.com/tgfb1-antibody-p-94593?filter_name=STJ95997 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

1. Rating:
Specificity:
ANTIBODY CUSTOMER REVIEW
Findings: “Strong signal, very cost effective
(1:2000), gives one unspecific band at 48-50 kD.”
– Gabor Kokeny, Semmelweis University.
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Protocol
Gel
Electrophoresis
12% polyacrylamide gel,
constant voltage 150 V, 60 min.
Membrane
Wash
1X TBST wash for 3 times.
Transfer 0.45 nm nitrocellulose
membrane, 1 piece of gel (wet
transfer), constant current 250
mA 60 min.
Secondary
Antibody
Probing
secondary antibody was added to
the solution of 1X TBST with 5%
skimmed milk powder with
1:2000 dilution ratio, for 1 hr.
Blocking 1X TBST with 5% skimmed milk
powder for 1 hr.
Membrane
Wash
1X TBST wash for 3 times.
Primary
Antibody
Probing
primary antibody was added to
the solution of 1X TBST with
2% BSA, 4˚C for overnight
Visualisation ECL visualization for 1 min (the
visualization time was
determined by actual result. It
was ensured that the
visualization time was identical
for the same antigen).
Model Number STJ95997
Antibody Name Anti-TGF-beta antibody
Application Western Blot
Species Rat
Tissue control and diabetic rat
kidney homogenate (30
ug / lane)
Dilution 1:2000
Secondary Antibody HRP goat anti-rabbit
Secondary Dilution 1:2000
Target bands at 40
kD, representing TGF-
beta were detected
by STJ95997 TGF-beta
antibody weakly in
control rat kidneys
but stronger signal
was detected in
diabetic rat kidney
(positive control).
(Figure: Lane 1:
control kidney; Lane
2: diabetic kidney).