Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

Immunofluorescence Antibody Customer Review for Laminin α-1 Polyclonal Antibody (STJ93888)

256 views

Published on

Laminin is known to play a role in the movement and organisation of embryonic cells into tissues during development. The laminin α-1 protein is encoded by the LAMA1 gene in humans. The laminin α-1 polyclonal antibody detects endogenous levels of laminin α-1 protein.

Published in: Science
  • Be the first to comment

  • Be the first to like this

Immunofluorescence Antibody Customer Review for Laminin α-1 Polyclonal Antibody (STJ93888)

  1. 1. HOW DID OUR ANTIBODY PERFORM? ANTIBODY CUSTOMER REVIEW: Laminin a-1 Polyclonal Antibody (STJ93888)
  2. 2. What was used? Primary antibody: STJ93888 Laminin a-1 Provider: St John's Laboratory Ltd. Dilution ratio: 1:50 Application: Immunofluorescence staining Materials for validation: Decellularised cornea Click for product data sheet PDF
  3. 3. What Was The Protocol? Treatment of Materials Tissue was sectioned at a 7 um thickness, and then fixed with 4% parafolmaldehyde and permeabilized with 1% Triton X-100. Fixation 4 % parafolmadehyde at room temperature for 20 minutes. Permeabilization 1% Triton X-100 at room temperature for 5 minutes. Blocking Blocking buffer (PBS with BSA and goat serum) for 1 hour. Primary Antibody Probing Primary antibody was added to the solution of blocking buffer (PBS with BSA and goat serum), at 4˚C overnight. Sample Wash The sample was washed with PBS with BSA and goat serum 5 times, each for 5 minutes. Secondary Antibody Probing Secondary antibody was added to the solution of blocking buffer (PBS with BSA and goat serum) with a 1:100 dilution ratio, for 1 hour. Sample Wash The sample was washed with PBS with BSA and goat serum 7 times, each for 5 minutes. Visualization Nikon Axiovert microscope.
  4. 4. What was the protocol? No. Antigen Primary antibody Primary antibody dilution ratio Secondary antibody dilution ratio 1 Decellularised cornea STJ93888 Laminin a-1 antibody 1:50 1:00
  5. 5. What were the results?
  6. 6. What did the customer think? Antibody Specificity: Antibody RPPA Rating: Testing results were provided independently by F. Abidin at Newcastle University. They stated “Visible laminin staining was observed from this tissue section.”
  7. 7. JOIN THE ANTIBODY VALIDATION PROJECT
  8. 8. 1. Order any STJ9 catalogued product using this online form. 2. Test it in your laboratory using your preferred method. 3. Return a fully completed review form to receive rewards. …in 3 simple steps…
  9. 9. @StJohnsLabs http://www.slideshare.net/stjohnslabs

×