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Antibody Customer Review for Phospho-AKT1 (T450) Polyclonal Antibody (STJ90168)


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Akt1 (a Serine/Threonine Protein Kinase) is involved in cell survival signal pathways. In response to PDGF (platelet-derived growth factor) Akt rapidly signals through its Serine/Threonine kinase by phosphorylating components that upregulate protein synthesis, prevent apoptosis and stimulate cell survival.

Published in: Science
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Antibody Customer Review for Phospho-AKT1 (T450) Polyclonal Antibody (STJ90168)

  1. 1. HOW DID OUR ANTIBODY PERFORM? CUSTOMER REVIEW: Phospho-Akt1 (T450) Polyclonal Antibody (STJ90168)
  2. 2. WHAT WAS USED? Primary Antibody: Phospho-Akt1 (T450) Polyclonal Antibody (STJ90168) Provider: St John's Laboratory Dilution ratio: 1:500 Application: Western Blot Materials for validation: C2C12 & RD cells
  3. 3. WHAT WAS THE PROTOCOL? Treatment of materials: Cells were lysed in RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% Triton X-100, 1% Sodium deoxycholate, 1% SDS, supplemented with protease inhibitors (PMSF 1mM, Leupeptin 1ug/ml, Aprotinin 1ug/ml, Pepstatin 1ug/ml). Proteins were reduced and denatured by heating at 100℃ for 5 min with subsequent cooling before use. Gel electrophoresis: 60ug/lane Transfer information: Nitocellulose membrane. Blocking: Blocked with 5% BSA for 0.5-1hr Primary Ab Application: Overnight at 4oC, 5% BSA in TBST. Secondary Ab Application: After washing with TBST 3 times membrane incubated secondary antibody for 1h at room temperature. Visualization: ECL kit
  5. 5. 1. Order any STJ9 catalogued product using this online form. 2. Test it in your laboratory using your preferred method. 3. Return a fully completed review form to receive rewards. …in 3 simple steps…
  6. 6. @StJohnsLabs