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Antibody Customer Review for Phospho-AKT1 (T450) Polyclonal Antibody (STJ90168)

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Akt1 (a Serine/Threonine Protein Kinase) is involved in cell survival signal pathways. In response to PDGF (platelet-derived growth factor) Akt rapidly signals through its Serine/Threonine kinase by phosphorylating components that upregulate protein synthesis, prevent apoptosis and stimulate cell survival.

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Antibody Customer Review for Phospho-AKT1 (T450) Polyclonal Antibody (STJ90168)

  1. 1. HOW DID OUR ANTIBODY PERFORM? CUSTOMER REVIEW: Phospho-Akt1 (T450) Polyclonal Antibody (STJ90168)
  2. 2. WHAT WAS USED? Primary Antibody: Phospho-Akt1 (T450) Polyclonal Antibody (STJ90168) Provider: St John's Laboratory Dilution ratio: 1:500 Application: Western Blot Materials for validation: C2C12 & RD cells
  3. 3. WHAT WAS THE PROTOCOL? Treatment of materials: Cells were lysed in RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% Triton X-100, 1% Sodium deoxycholate, 1% SDS, supplemented with protease inhibitors (PMSF 1mM, Leupeptin 1ug/ml, Aprotinin 1ug/ml, Pepstatin 1ug/ml). Proteins were reduced and denatured by heating at 100℃ for 5 min with subsequent cooling before use. Gel electrophoresis: 60ug/lane Transfer information: Nitocellulose membrane. Blocking: Blocked with 5% BSA for 0.5-1hr Primary Ab Application: Overnight at 4oC, 5% BSA in TBST. Secondary Ab Application: After washing with TBST 3 times membrane incubated secondary antibody for 1h at room temperature. Visualization: ECL kit
  4. 4. JOIN THE ANTIBODY VALIDATION PROJECT
  5. 5. 1. Order any STJ9 catalogued product using this online form. 2. Test it in your laboratory using your preferred method. 3. Return a fully completed review form to receive rewards. …in 3 simple steps…
  6. 6. @StJohnsLabs http://www.slideshare.net/stjohnslabs

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