Succeed Cestaro Presentation 031209

1,382 views

Published on

Succeed Cestaro Presentation 031209

Published in: Business, Health & Medicine
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total views
1,382
On SlideShare
0
From Embeds
0
Number of Embeds
10
Actions
Shares
0
Downloads
10
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

Succeed Cestaro Presentation 031209

  1. 1. N-Oleoyl-Phosphatidyl-Ethanolamine (NOPE) + EGCG: Scientific & Clinical Support For Appetite Suppression & Weight Management 4463 White Bear Pkwy., Suite 105 White Bear Lake, MN 55110 © Chemi Nutra 2009
  2. 2. Succeed™ <ul><li>Next generation nutritional supplement compound for advanced appetite control and weight management. </li></ul><ul><li>Proprietary composition of </li></ul>N-oleoyl-phosphatidyl-ethanolamine epigallocatechin gallate from standardized green tea extract. and EGCG NOPE
  3. 3. Proprietary Composition Of PhosphoLean™ NOPE+EGCG <ul><li>Gastro-protection of active constituents with release in the small intestine. </li></ul><ul><li>Increased bioavailability. </li></ul><ul><li>Increased intestinal levels of NOPE, thus of NOE. </li></ul><ul><li>Markedly amplified activities of both components, enhancing the weight management property of Succeed™. </li></ul>
  4. 4. Epigallocatechin Gallate (EGCG) <ul><li>Green Tea Extract activities: </li></ul><ul><li>Antioxidant. </li></ul><ul><li>Reduction of excess blood cholesterol. </li></ul><ul><li>Improvement of insulin action. </li></ul><ul><li>Helps to control weight homeostasis. </li></ul><ul><li>Increases fat oxidation and thermogenesis. </li></ul><ul><li>EGCG most active of green tea catechins: </li></ul><ul><li>Enhances energetic metabolism. </li></ul><ul><li>Increases catabolism of lipids and decreases accumulation of triglycerides. </li></ul><ul><li>Decreases blood glucose. </li></ul><ul><li>Positively affects cardiovascular system. </li></ul>
  5. 5. N-Oleoyl-Phosphatidyl-Ethanolamine (NOPE) <ul><li>Natural occurring phospholipids in cellular membranes. </li></ul><ul><li>Present in some foods: soy, eggs, milk, chocolate. </li></ul><ul><li>Produced and metabolized in man. </li></ul><ul><li>Precursor of N-oleoyl-ethanolamine (NOE). </li></ul>
  6. 6. N-Acyl-Ethanolamine <ul><li>N-acyl-ethanolamine or acyl-ethanolamides. </li></ul><ul><li>Cell mediators synthesized on demand, starting from phospholipid precursors. ( N-Acyl-Phosphatidyl-Ethanolamine ): </li></ul><ul><ul><li>N-oleoyl-ethanolamine (NOE) </li></ul></ul><ul><ul><li>N-palmitoyl-ethanolamine </li></ul></ul><ul><ul><li>N-arachidoyl-ethanolamine (Anandamide) </li></ul></ul>
  7. 7. N-Acyl-Ethanolamine & Appetite Control <ul><li>Lipid mediator involved in peripheral regulation of feeding. </li></ul><ul><li>NOE </li></ul><ul><li>Peripheral action. </li></ul><ul><li>Anorectic action. </li></ul><ul><li>Anandamide </li></ul><ul><li>Central and peripheral action. </li></ul><ul><li>Orexigenic action. </li></ul>NOE ANANDAMIDE
  8. 8. NAPE and NAE Intestinal Levels <ul><li>Starvation and re-feeding influence NAPE and NAE levels in the epithelium of intestinal mucosa. </li></ul><ul><li>( Duodenum ) </li></ul>
  9. 9. N-Acyl-Phosphatidyl-Ethanolamine (NAPE) Intestinal Levels Pmol / mmol P G. Peterson et al. Biochimica Biophysica Acta (2006) 1761:143 Measurements of individual N-Acyl species of NAPE in liquid extracts of tissue samples were carried out by GC-MS in the presence of deutero internal standards, after determination of lipid phosphorus (P) in each sample. Values are means ± SEM ( n =6). *P<0.05 when applying one way ANOVA followed by Tukey’s Post Hoc test. **P<0.01 when applying one way ANOVA followed by Tukey’s Post Hoc test. 1.4 + 0.09 1.3 + 0.09 1.5 + 0.21* 1.1 + 0.07 C20:4 NArPE 39 + 2.4 56 + 5.2 14 + 1.1** 43 + 6.7 C18:2 4.5 + 0.40 4.3 + 0.28 4.1 + 0.36 3.7 + 0.12 C18:1 n-7 8.2 + 0.45 11 + 11 3.4 + 0.19** 8.6 + 1.4 C18:1 n-9 NOPE 7.8 + 0.36 8.1 + 0.23 7.9 + 0.60 8.0 + 0.3 C18:0 20 + 0.97 20 + 0.70 15 + 1.1* 19 + 0.7 C16:0 Starved 24h and re-fed 4h Starved 24h and re-fed 1h Starved 24h Free- feeding ACYL GROUP N-Acyl composition of NAPE in rat intestine after starvation and re-feeding:
  10. 10. Mechanism Of Action N-Oleoyl-Ethanolamine (NOE) <ul><li>Activation of Intestinal Receptors: </li></ul><ul><li>PPAR  </li></ul><ul><li>GPR119 </li></ul>ANOREXIC EFFECT REDUCTION of FOOD INTAKE
  11. 11. PPAR  (Peroxisome Proliferator Activated Receptor) <ul><li>Nuclear receptors modulating genetic expression. </li></ul><ul><li>Naturally occurring in intestine, liver, kidney, heart, muscles and adipocytes. </li></ul><ul><li>Regulate differentiation of pre-adipocytes into adipocytes. </li></ul><ul><li>Regulate lipolysis. </li></ul>
  12. 12. GPR119 <ul><li>Protein G coupled membrane receptors. </li></ul><ul><li>Mainly expressed in pancreas  cells and in gastro-intestinal tract. </li></ul><ul><li>Metabolic results: </li></ul><ul><li>Improved glucose handling. </li></ul><ul><li>Slowed diabetes progression. </li></ul><ul><li>Weight loss. </li></ul>
  13. 13. Succeed™ Mechanism Of Action EGCG NOE EFFECTS Enhanced release of endothelium NO. Reduction of LDL oxidation. Improved vessel integrity. Improved insulin sensitivity. GPR119 activation. Improved glucose handling. Liver UCP2-UCP3 activation. Adipocyte PPAR  receptor activation. Increased lipidic catabolism. Boosts thermogenesis. Intestinal PPAR  receptor activation. Increased sense of satiety. Mechanism Mechanism
  14. 14. Succeed™ Animal Investigational Studies <ul><li>“ N-oleoyl-phosphatidylethanolamine reduced food intake and body weight of dietary rats ameliorating their antioxidant status.” </li></ul>B. Cestaro et al. Arc Sci Med 2005; 164: 101-107
  15. 15. Study Design <ul><li>12 rats fed with standard diet (S-diet) </li></ul><ul><li>12 rats fed with caloric diet inducing obesity (OP-diet) </li></ul><ul><li>12 rats fed with OP-diet + NOPE-EGCG (OP-diet + Succeed™) </li></ul>
  16. 16. Results <ul><li>At 14 weeks, Group 3 (OP+NOPE diet) food intake was significantly lower than Group 2 (OP-diet). </li></ul>Weeks Food intake (g) Figure shows food intake in the treated groups.
  17. 17. Results OP diet + Succeed TM Reduction of weight gain and total body fat. OP-diet+Succeed™ OP-diet S-diet 21.6 + 0.8 25.3 + 0.7 18.1 + 0.6 % Body fat 17.3 + 0.5 17.1 + 0.4 19.2 + 0.4 % Body proteins 57.1 + 0.6 53.4 + 0.5 58.6 + 0.6 % H 2 O 344.3 + 8.4 367.0 + 8.3 311.6 + 7.6 Final weight at 14 weeks 236.8 + 7.6 258.9 + 7.5 217.6 + 6.8 Weight at 10 weeks 106.06 + 0.9 106.65 + 1.1 106.1 + 1.0 Starting weight at 6 weeks GROUP 3 GROUP 2 GROUP 1
  18. 18. Results OP diet + Succeed TM Improvement of glucose, triglycerides and cholesterol plasma levels. OP-diet+Succeed™ OP-diet S-diet 1.04 + ±0.06 1.06 + 0.08 1.01 + 0.07 Cholesterol HDL 1.77 + 0.13 2.17 + 0.16 1.64 + 0.14 Total cholesterol 1.24 + ±0.12 1.78 + ±0.21 b 1.06 + 0.11 Triglycerides 9.65 + 0.59 10.59 + 0.55 9.89 + 0.48 Glucose GROUP 3 GROUP 2 GROUP 1
  19. 19. Results OP diet + Succeed TM Decrease of lipidic peroxidation (TBARS) and enhanced tissue antioxidant defense. OP-diet+Succeed™ OP-diet S-diet 140.81 + 7.98 128.42 + 9.15 154.67 + 10.36 Liver ATP (nmol/mg proteins) 30.74 + 2 26.17 + 1.47 35.98 + 2.83 Liver GSH (nmol/10 5 cells) 34.96 + 3.21 37.05 + 0.48 24.83 + 3.12 Liver TBARS (nmol/g tissue) 3.14 + 0.5 4.43 + 0.48 9.85 + 0.32 Plasma TBARS (nmol/ml) GROUP 3 GROUP 2 GROUP 1
  20. 20. Succeed™ Human Clinical Study “ Administration of a dietary supplement (N-oleoyl-phosphatidyl-ethanolamine and epigallocatechin-3-gallate formula) enhanced compliance with diet in healthy overweight subjects: a randomized controlled trial” M. Rondanelli et al. British Journal of Nutrition 2009; 101 :457-464
  21. 21. Study Design 138 Subjects (F=106 / M=32) GROUP 1 NOPE-EGCG complex N 71 (F=53 / M=18) GROUP 1 PLACEBO N 67 (F=53 / M=14) COMPLETED N 67 (F=50 / M=17) DROPPED OUT N 4 (F=3 / M=1) COMPLETED N 49 (F=39 / M=10) DROPPED OUT N 18 (F=14 / M=4)
  22. 22. Results COMPLETED DROP OUT SUBJECTS 94% 4 71 Succeed™ 73% 18 67 Placebo <ul><li>Succeed™ treated subjects complied with diet >94%, with a considerable difference with respect to placebo group. </li></ul><ul><li>High drop out placebo group: Failure to follow the diet because of severe hunger. </li></ul>94% 4 71 PhosphoLean™
  23. 23. Results <ul><li>Succeed™ positively affected mood: </li></ul><ul><ul><li>Enhanced sense of satiety / fullness . </li></ul></ul><ul><ul><li>Enhanced capacity to pursue a low-calorie diet. </li></ul></ul><ul><ul><li>Helped change eating habits. </li></ul></ul>1.42 -6.41 -4.21 Succeed™ 0.63 -1.24 -1.36 Placebo 1.42 -6.41 -4.21 Succeed™ HABER TEST BES BDI BDI = Beck Inventory Scale assesses depressive symptoms. BES = Binge Eating scale indicative of need for between meal eating. Haber Test = Sense of satiety during all treatment.
  24. 24. Results <ul><li>Succeed™ significantly improved glucose metabolic parameters. </li></ul>-0.59 -18.50 Succeed™ -0.01 +2.58 Placebo -0.59 -18.50 Succeed™ HOMA INSULIN (pmol/L) HOMA = Homeostasis Model Assessment for insulin resistance assessment.
  25. 25. Results <ul><li>Succeed™ improved anthropometric parameters. </li></ul>-2.34 -2.67 Placebo -3.45 -3.28 Succeed™ Waist Circumference (cm) Weight (kg) Anthropometric measurements: Body mass index (BMI). Waist-to-hip ratio. Waist circumference. Evaluation in clinical settings was used for rough estimation of abdominal fat volumes. Abdominal obesity is a Metabolic Syndrome health risk. -3.45 -3.28 Succeed™
  26. 26. <ul><li>Significantly helped committed people stay on their stressful low-calorie diets. </li></ul><ul><li>Enhanced sense of satiety / appetite suppression. </li></ul><ul><li>Decreased depressive symptoms. </li></ul><ul><li>Decreased binge eating severity. </li></ul><ul><li>Provided favorable changes in insulin resistance. </li></ul>Succeed™ Clinical Study Summary
  27. 27. Succeed™ Supplement Facts <ul><li>Amount per serving (2 softgel capsules) </li></ul><ul><li>Proprietary blend of N-oleoyl-phosphatidyl-ethanolamine (NOPE) and epigallocatechin gallate (EGCG) complex (PhosphoLean™) 690 mg *containing NOPE 170 mg* EGCG 120 mg* *Daily Value not established </li></ul><ul><li>Ingredients: Soy oil, soy phospholipids enriched with N-oleoylphospatidylethanolamine (NOPE), gelatin, green tea extract, polyglyceryl oleate, glycerol, sorbitol, titanium dioxide, iron oxide, yellow # 13 and blue # 5 </li></ul>
  28. 28. A successful diet is one you can stay on.
  29. 29. Questions? Thank you! 4463 White Bear Pkwy., Suite 105 White Bear Lake, MN 55110 © Chemi Nutra 2009

×