SAFS Aquaculture

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Overview presentation on aquaculture related research

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SAFS Aquaculture

  1. 1. Genomic approaches in Aquaculture Research Steven Roberts Assistant Professor Friday, May 15, 2009
  2. 2. Overview Big Fish Myostatin Regulation Better Shellfish Disease Resistance Bad Bugs Pathogen Virulence Friday, May 15, 2009
  3. 3. Myostatin Friday, May 15, 2009
  4. 4. Does myostatin function in a similar manner in fish as it does in mammals? = Friday, May 15, 2009
  5. 5. Approach Identify molecules MSTN (mature peptide) that will decrease biological activity LAP Friday, May 15, 2009
  6. 6. Identification of Myostatin Interacting Proteins Approach: Yeast two hybrid system Friday, May 15, 2009
  7. 7. Results Yeast-two-Hybrid LAP 1. Telethonin 2. Friday, May 15, 2009
  8. 8. Future Work • Co-IP targets identified with Y2H • Sequence proteins from muscle extract captured via co-IP • Primary muscle culture experiments • Large scale recombinant protein expression and expose juvenile fish Friday, May 15, 2009
  9. 9. Acknowledgements FUNDING This project is supported by National Research Initiative Competitive Grant no. 2005-35206-15261 from the USDA Cooperative State Research, Education, and Extension Service. Technical Assistance Christina Holmes Sam White Friday, May 15, 2009
  10. 10. Overview Big Fish Myostatin Regulation Better Shellfish Oysters Disease Resistance Abalone Bad Bugs Pathogen Virulence Friday, May 15, 2009
  11. 11. Disease MVineyard ? Resistance flickr | cpurrin1 Crassostrea virginica Roxannna Smolowitz Rick Karney Friday, May 15, 2009
  12. 12. •Mortality •Disease •Immune Response Friday, May 15, 2009
  13. 13. C. virginica Friday, May 15, 2009
  14. 14. Abalone Friday, May 15, 2009
  15. 15. Naive Disease Pressure Glenn VanBlaricom, Carolyn Friedman Friday, May 15, 2009
  16. 16. Carolyn Friedman Friday, May 15, 2009
  17. 17. H. cracherodii Friday, May 15, 2009
  18. 18. H. cracherodii Friday, May 15, 2009
  19. 19. Summary • Just as with oysters, processes of natural selection have resulted in populations with increased tolerance. • Important mechanisms involved in the immune response to WS are associated with initial pathogen recognition. Friday, May 15, 2009
  20. 20. Overview Big Fish Myostatin Regulation Better Shellfish Disease Resistance Bad Bugs Vibrio Pathogen Virulence tubiashii Friday, May 15, 2009
  21. 21. How do changes in the environment influence Vibrio tubiashii physiology? Friday, May 15, 2009
  22. 22. Approach - Protein Expression Protein Extraction 2D gels ID differences Friday, May 15, 2009
  23. 23. Differential Expression Friday, May 15, 2009
  24. 24. GO Annotations biosynthesis Chart 6 response to endogenous stimulus transport ion transport signal transduction Control cell communication transcription protein metabolism cell cycle carbohydrate metabolism protein biosynthesis cell organization and biogenesis response to external stimulus amino acid and derivative metabolism morphogenesis protein modification generation of precursor metabolites and energy death response to abiotic stimulus Low Oxygen cell death cell differentiation cell homeostasis nucleic acid metabolism secondary metabolism catabolism lipid metabolism DNA metabolism response to stress 0 0.025 0.050 0.075 0.100 Friday, May 15, 2009
  25. 25. V. tubiashii Friday, May 15, 2009 V. tubiashii + live oysters V. tubiashii + sterile oysters Oysters
  26. 26. pI MW 3.7 29k 4.5 49k MW 4.8 26k Friday, May 15, 2009
  27. 27. Vibrio tubiashii 226 million bp Friday, May 15, 2009
  28. 28. Future Direction • Comparative microbial analysis to determine species specific responses to environmental change • Coordinate with experimental and environmental sampling - relevance • Characterize the host response in relation to pathogen physiology Friday, May 15, 2009
  29. 29. Thank you for more information fish.washington.edu/genefish sr320@washington.edu Friday, May 15, 2009

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