Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

Parasites Lab 08


Published on

Published in: Health & Medicine, Lifestyle
  • Be the first to comment

Parasites Lab 08

  1. 1. Shellfish Parasites & Pathogens
  2. 2. Definitions <ul><li>Disease : an alteration from the normal state </li></ul><ul><li>Parasite : an organism that is metabolically dependent on its host (gets its energy or food from its host) </li></ul><ul><li>Infectious : transmissible (e.g. virus, bacteria, protozoa) </li></ul><ul><li>Pathogen : an organism that is able to cause disease. Parasites, under certain conditions, may cause disease. </li></ul><ul><li>Virulence : characteristics of the pathogen that allow it to infect, multiply and spread in or among hosts </li></ul><ul><li>Pathogenicity : the ability to cause disease within a host </li></ul><ul><li>Presence of a parasite does not equate to presence of a disease </li></ul>
  3. 3. Development of disease Environment Host Pathogen Host Disease Environment Pathogen
  4. 4. What is a Parasite? <ul><li>Ectoparasite </li></ul><ul><li>Endoparasite </li></ul>
  5. 5. Barnacle parasites just don’t quit! <ul><li>Rhizocephalan – decapod parasite </li></ul><ul><li>Inhibits molting </li></ul><ul><li>Looses ability to reproduce </li></ul><ul><li>Males gain female characteristics & appearance </li></ul>
  6. 7. Bopyrid Isopod
  7. 9. Parasitic copepods <ul><li>1,700 species infecting fish </li></ul><ul><li>Some share many morphological adaptations of free-living counterparts (ie: sea lice) </li></ul><ul><li>Others permanently anchored; penetrate deep into host tissue. Jointed appendages and external segmentation greatly reduced </li></ul>
  8. 11. Withering Syndrome <ul><li>Chronic wasting disease – symptoms include: weakness, lethargy, diminished pedal mass, mantle retraction, poor gonadal development, and DEATH </li></ul>
  9. 12. <ul><li>Etiological agent: “ Candidatus Xenohaliotis Californiesis ” </li></ul><ul><li>Rickettsiales-like prokaryote (RLP) </li></ul><ul><li>Intracellular bacteria that inhabits the epithelial cells of the GI tract from the esophagus to the digestive gland </li></ul><ul><li>Forms basophilic inclusions (large cytoplasmic bacterial colonies) </li></ul><ul><li>DG metaplasia (transformation of tissue as adaptive response) & degeneration (can’t secrete digestion enzymes) </li></ul><ul><li>Abalone catobolizes foot myofibers for energy </li></ul>
  10. 16. DERMO <ul><li>Causative agent: Perkinsus marinus (closely related to dinoflagellates – what phylum?) </li></ul><ul><li>Species affected: Eastern oyster ( Crassostrea virginica ) mussel ( Mytilus edulis ) Pacific oyster ( Crassostrea gigas ) sand gaper mussel ( Mya arenaria ) Suminoe oyster ( Crassostrea ariakensis ) </li></ul><ul><li>Clinical signs: poor condition, gaping, shrinkage of mantle, retarded growth, emaciation </li></ul><ul><li>Gross signs: occasional pustules in soft tissue, pale digestive gland </li></ul><ul><li>Epidemiology: </li></ul><ul><li>Proliferation associated with water temperatures above 20°C </li></ul><ul><li>Low salinity (below 9 ppt) prevents clinical disease </li></ul><ul><li>Suggested fecal-oral transmission </li></ul><ul><li>Mortalities of up to 95% of C. virginica have been observed in the second summer following transfer to a disease endemic area </li></ul><ul><li>Infection levels increase during spawning and under the stress of oxygen depletion </li></ul><ul><li>Prevalence and intensity of infection are greatest in oysters more than one year old and at depths ≥ 1m </li></ul><ul><li>Exposure to pollutants will increase the prevalence of infection </li></ul>
  11. 19. White Spot Syndrome <ul><li>Highly lethal & contagious viral infection of crustaceans esp. penaeid shrimp; can wipe out an entire population in a few days </li></ul><ul><li>Causative agent : White Spot Syndrome Virus (WSSV) </li></ul><ul><li>Clinical signs : sudden reduction in food consumption, lethargy, loose cuticle and often reddish discoloration, and the presence of white spots of 0.5 to 2.0 mm in diameter on the inside surface of the carapace, appendages and cuticle over the abdominal segments. </li></ul><ul><li>Transmission : mainly oral - water borne routes (horizontal transmission) and passed onto offspring (vertical transmission) </li></ul><ul><li>Histopathological changes : </li></ul><ul><ul><li>prominent intranuclear inclusions </li></ul></ul><ul><ul><li>cellular degeneration with hypertrophied nuclei and chromatin margination </li></ul></ul>