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Does DNA methylation facilitate
phenotypic plasticity in marine
invertebrates?
Steven Roberts
School of Aquatic and Fisher...
Shameless plug
onsnetwork.org/eimd
@EIMD_fhl
Shameless plug
onsnetwork.org/eimd
@EIMD_fhl
Does DNA methylation facilitate
phenotypic plasticity in marine
invertebrates?
Open Science
• You are free to Share!
• Our lab practices open notebook
science
• Data, Preprints, Proposals, Slidedecks a...
commercially important traits
Lab Background
commercially important traits
modified from
Lab Background
modified from
modified from
modified from
Today
modified from
1
2
3
Histone Modification
Epigenetics
short RNAs
DNA Methylation
Photo credit: Flickr, Creative Commons, he-boden
Epigenetics
Epigenetics
m
m
DNA Methylation
Function?
Non-
Vertebrates?
landscape and function is very different than
what is observed in vertebrates
Non-
Vertebrates?
landscape and function is very different than
what is observed in vertebrates
Non-
Vertebrates?
landscape and function is very different than
what is observed in vertebrates
Absent in
several
model
organisms
Function?Oysters?
mosaic
associated with gene bodies
Roberts and Gavery 2012
in silico analysis
Roberts and Gavery 2012
in silico analysis
Roberts and Gavery 2012
in silico analysis
Two Lineages
-
Sperm +
Larvae
(72h & 120h)
Claire Olson
Whole Genome BS-Seq
Claire Olson
Differentially Methylated Loci predominant in
Transposable Elements
Claire Olson
github.com/sr320/olson-dev
Summary
• Sparsely (~16 %) methylated genome
• Gene body methylation
• Function specific
•TEs are not hypermethylated acros...
Role?
In species that experience a diverse range of
environmental conditions, processes have evolved to
increase the number of p...
Roberts and Gavery 2012
in silico analysis
Stochastic Variation
housekeeping
response to
change
Mackenzie Gavery
housekeeping
response to
change
Targeted
Regulation
Mackenzie Gavery
Mackenzie Gavery
Transposable Elements providing increased diversity?
modified from
Beginning to
test these
Hypotheses
Very new data
Environment and gene expression
stochastic or targeted?
Very new data
Environmental impact (Estrogens)
Very new data
Ocean Acidification
Selection
Environmental Impact
Katie Lotterhos
Very new data
Heritability
Plasticity
Local Adaptation
Genetics
versus
Epigenetics
Common Garden Experiment
Acknowledgements
Mackenzie Gavery
Claire Ellis
Sam White
Bill Howe
Dan Halperin
EPA
STAR
DNA methylation
robertslab.infosl...
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
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Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?

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Presentation given at the Friday Harbor Laboratories on August 13th, 2014

Published in: Education
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Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?

  1. 1. Does DNA methylation facilitate phenotypic plasticity in marine invertebrates? Steven Roberts School of Aquatic and Fishery Sciences robertslab.info @sr320
  2. 2. Shameless plug onsnetwork.org/eimd @EIMD_fhl
  3. 3. Shameless plug onsnetwork.org/eimd @EIMD_fhl
  4. 4. Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
  5. 5. Open Science • You are free to Share! • Our lab practices open notebook science • Data, Preprints, Proposals, Slidedecks available FigShare, GitHub, and lab website (robertslab.info) These slides plus links @ robertslab.info
  6. 6. commercially important traits Lab Background
  7. 7. commercially important traits modified from Lab Background
  8. 8. modified from
  9. 9. modified from
  10. 10. modified from
  11. 11. Today modified from 1 2 3
  12. 12. Histone Modification Epigenetics short RNAs DNA Methylation
  13. 13. Photo credit: Flickr, Creative Commons, he-boden Epigenetics
  14. 14. Epigenetics
  15. 15. m m DNA Methylation
  16. 16. Function?
  17. 17. Non- Vertebrates? landscape and function is very different than what is observed in vertebrates
  18. 18. Non- Vertebrates? landscape and function is very different than what is observed in vertebrates
  19. 19. Non- Vertebrates? landscape and function is very different than what is observed in vertebrates
  20. 20. Absent in several model organisms
  21. 21. Function?Oysters?
  22. 22. mosaic associated with gene bodies
  23. 23. Roberts and Gavery 2012 in silico analysis
  24. 24. Roberts and Gavery 2012 in silico analysis
  25. 25. Roberts and Gavery 2012 in silico analysis
  26. 26. Two Lineages - Sperm + Larvae (72h & 120h) Claire Olson Whole Genome BS-Seq
  27. 27. Claire Olson Differentially Methylated Loci predominant in Transposable Elements
  28. 28. Claire Olson github.com/sr320/olson-dev
  29. 29. Summary • Sparsely (~16 %) methylated genome • Gene body methylation • Function specific •TEs are not hypermethylated across genome • Preliminary evidence indicates DMRs are predominant in TEs
  30. 30. Role?
  31. 31. In species that experience a diverse range of environmental conditions, processes have evolved to increase the number of potential phenotypes in a population in order to improve the chances for an individual’s survival.
  32. 32. Roberts and Gavery 2012 in silico analysis
  33. 33. Stochastic Variation housekeeping response to change
  34. 34. Mackenzie Gavery
  35. 35. housekeeping response to change
  36. 36. Targeted Regulation
  37. 37. Mackenzie Gavery
  38. 38. Mackenzie Gavery
  39. 39. Transposable Elements providing increased diversity?
  40. 40. modified from Beginning to test these Hypotheses
  41. 41. Very new data Environment and gene expression stochastic or targeted?
  42. 42. Very new data Environmental impact (Estrogens)
  43. 43. Very new data Ocean Acidification Selection Environmental Impact Katie Lotterhos
  44. 44. Very new data Heritability Plasticity Local Adaptation Genetics versus Epigenetics Common Garden Experiment
  45. 45. Acknowledgements Mackenzie Gavery Claire Ellis Sam White Bill Howe Dan Halperin EPA STAR DNA methylation robertslab.infoslides, data & more @

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