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Fungi Experiment


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Fungi Experiment

  1. 1. Review of slides in mycology Pictures c/0 Karentan.Content c/o chumtemp. OLFU Med II 2008
  2. 2. Dermatophytosis/ cutaneous mycoses “ Tineas”
  3. 3. FUNGIFACTS: DERMATOPHYTES Genus Species Macroconidia Microconidia Disease(s) Produced Epidermophyton floccosum Numerous Smooth- walled Absent Tinea corporis, pedis, ungium, cruris Microsporum canis Numerous thick walled Rough POINTED ENDS (+)Spiny tips Rare Tinea capitis Tinea corporis Microsporum gypseum Numerous thiN walled Rough BLUNT ENDS Rare Tinea capitis Tinea corporis Trichophyton mentagrophytes Rare, thin walled, smooth *Hypha: COILED Grape-like Tinea pedis, ungium, cruris, barbae Trichophyton Rubrum Absent Teardrop Tinea corporis, pedis, ungium, cruris
  4. 5. Epidermophyton floccosum Numerous, smooth walled MACROconidia
  5. 6. <ul><li>Thick-walled, pointed end MACROconidia </li></ul>Microsporum canis With spiny tips
  6. 7. Microsporum gypseum <ul><li>Blunt end MACROconidia </li></ul>
  7. 8. Trichophyton mentagrophytes Grape-like clusters (“en grappe”) microconidia macroconidia *Coiled hyphae
  8. 9. Trichophyton rubrum <ul><li>Tear drop (enthyrse) microcondia </li></ul>
  9. 10. systemic mycosis Histoplasmosis
  10. 11. Histoplasma capsulatum tuberculate MACROconidia <ul><li>FUNGIFACTS </li></ul><ul><li>Histoplasmosis </li></ul><ul><li>Laboratory Diagnosis: </li></ul><ul><li>KOH: organisms found inside histiocytes </li></ul><ul><li>Wright/Giemsa </li></ul><ul><li>Culture: SDA, BAP (Smith & Goodman) </li></ul><ul><li>Serology </li></ul><ul><ul><li>Skin test: Histoplasmin Ag </li></ul></ul><ul><ul><li>CF </li></ul></ul>
  11. 12. opportunistic mycoses <ul><li>Aspergillosis </li></ul><ul><li>Candidiasis </li></ul><ul><li>Cryptpococcosis </li></ul><ul><li>Mucormycosis </li></ul><ul><li>Penicilliosis </li></ul>
  12. 13. Aspergillus fumigatus conidiophore conidia vesicle Biseriate phialide - phialide - metula <ul><li>FUNGIFACTS </li></ul><ul><li>Aspergillosis </li></ul><ul><li>(+) Fungus ball - spherical mass of intertwined septate branching hyphal elements </li></ul><ul><li>Laboratory Diagnosis: </li></ul><ul><li>septate hyphae that branch at regular intervals seen in clinical specimens </li></ul>
  13. 14. Candida albicans <ul><li>FUNGIFACTS </li></ul><ul><li>*Chronic Mucocutaneous Candidiadis </li></ul><ul><li>Laboratory Diagnosis: </li></ul><ul><ul><li>(+) germ tube test (rapid diagnosis) </li></ul></ul><ul><ul><li>(+)Budding yeast cells </li></ul></ul><ul><ul><li>(+)pseudohyphae </li></ul></ul><ul><ul><li>(+)septate hyphae </li></ul></ul>Budding yeasts Germ tubes
  14. 15. Cryptococcus neoformans Encapsulated yeast <ul><li>FUNGIFACTS </li></ul><ul><li>Cryptococcosis </li></ul><ul><li>Laboratory Diagnosis: </li></ul><ul><li>India ink: encapsulated yeast </li></ul>
  15. 16. Mucor sp. <ul><li>Sporangium </li></ul><ul><li>*sporangiospores inside </li></ul><ul><li>FUNGIFACTS </li></ul><ul><li>Mucormycosis </li></ul><ul><li>Laboratory Diagnosis: </li></ul><ul><li>(+)coenocytic hyphae </li></ul><ul><li>(+) sporangium containing sporangiospores </li></ul><ul><li>* Ubiquitous in the environment and encountered as contaminants </li></ul>sporangiospores Coenocytic (aseptate) hyphae
  16. 17. Rhizopus sp. sporangiospores sporangium rhizoids <ul><li>FUNGIFACTS </li></ul><ul><li>Mucormycosis </li></ul><ul><li>Laboratory Diagnosis: </li></ul><ul><li>(+) RHIZOIDS </li></ul><ul><li>(+) sporangium containing sporangiospores </li></ul>
  17. 18. Penicillium sp. Chains of conidia Branching conidiophore Phialides
  18. 19. Subcutaneous mycoses Sporotrichosis Chromomycosis
  19. 20. Sporothrix schenckii Daisy-like microconidia <ul><li>FUNGIFACTS </li></ul><ul><li>Sporotrichosis (Lymphocutaneous) </li></ul><ul><li>Dimorphic </li></ul><ul><ul><li>Yeast: cigar body (budding yeast cells) </li></ul></ul><ul><ul><li>Mold: numerous micro conidia in a rosette/daisy pattern </li></ul></ul>
  20. 21. Cladosporium carrionii Long chain of conidia <ul><li>FUNGIFACTS </li></ul><ul><li>Chromomycosis </li></ul><ul><li>Dematiaceous </li></ul><ul><ul><li>Mold: branching conidiophore </li></ul></ul><ul><ul><li>Chains of conidia </li></ul></ul><ul><ul><li>Septate hypha </li></ul></ul>
  21. 22. Phialophora verrucosa Flask-shaped conidipohore <ul><li>FUNGIFACTS </li></ul><ul><li>Chromomycosis </li></ul><ul><li>Verrucous dermatitis </li></ul><ul><li>Dematiaceous </li></ul><ul><ul><li>Septate hypha w/ short conidiophore & collarate mass of conidia </li></ul></ul>
  22. 23. Fonseca pedrosoi Club-shaped conidiophore Pattern of sporulation: acrotheca Conidia @ lateral sides