Microsoft power point พันธุศาสตร์และเทคโนโลยีทาง dna

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Microsoft power point พันธุศาสตร์และเทคโนโลยีทาง dna

  1. 1. By Thanyamon C. DNA  1
  2. 2. (BIOTECHNOLOGY) Œ Œ  By Thanyamon C. Œ  Œ   Œ Œ ˜ , ‹ ‹ , , DNA Technology 2
  3. 3. (GENETIC ENGINEERING) Œ DNA Œ recombinant DNA Recombinant DNA Œ DNA š ‹ By Thanyamon C. DNA Œ  Œ Œ Œ 3
  4. 4.  (RESTRICTION ENZYME) Hamilton Smith ŒŒ  š ˜ Œ  By Thanyamon C. 1,200 ‹ ‹ ‹ Œ ‹ 5’ → 3’ Œ Œ 2 ‹ DNA Œ DNA ‹ (sticky end) ‹Œ ‹ (blunt end) 4
  5. 5. By Thanyamon C. 5 
  6. 6. By Thanyamon C. 6 ‹
  7. 7.  (DNA LIGASE ENZYME) š  Œ ‹ DNA ‹ Œ  ‹ 2 By Thanyamon C. DNA Œ ‹ Œ ‹ DNA ‹ ‹ Œ Œ Œ Œ restriction enzyme ‹ DNA Œ DNA (Recombinant DNA) 7
  8. 8. RECOMBINANT DNA By Thanyamon C. 8
  9. 9. (CLONE) DNA Œ Œ ‹ Œ‹ By Thanyamon C. DNA (DNA cloning) DNA (gene cloning) DNA š 9
  10. 10. (plasmid) DNA š ‹ ‹ By Thanyamon C. DNA (vector) DNA Œ ‹ DNA  DNA Œ Œ Œ Œ ‹ 10 ŒŒ ‹ Œ Œ
  11. 11. By Thanyamon C. 11 DNA CLONING
  12. 12. PCR PCR (polymerase chain reaction) ‹ DNA Œ ‹  By Thanyamon C. Œ ˜ ‹ thermocycler š Œ Œ  Œ DNA polymerase Œ Œ Œ ‹ Œ 12
  13. 13. PCR ( ‹ ) Œ Œ DNA ‹ š ‹ DNA Œ By Thanyamon C.  (primer) š DNA ‹ DNA Œ š Œ Œ DNA Nucleotide 4 ATCG DNA polymerase  pH Œ 13
  14. 14. By Thanyamon C. 14
  15. 15. By Thanyamon C. 15 PCR
  16. 16. PCR ( ‹ )  PCR DNA Œ DNA Œ By Thanyamon C. Œ Œ (‹ Œ PCR ‹ Œ Œ ‹) Œ  Œ DNA Œ DNA Œ ‹ ‹ DNA ‹  16
  17. 17.  DNA By Thanyamon C. Gel electrophoresis DNA ‹ Œ ‡ ‹ š ‹ Œ ‹ agarose gel polyacrylamide gel DNA ( -) Œ (anode) 17 DNA ‹ Œ‹ ‹ Œ Œ ‹
  18. 18.  DNA ( ‹ ) DNA ‹ ‹ Œ Œ Œ Œ ethidium bromide By Thanyamon C. DNA Œ ‹ Œ Œ DNA Œ Œ DNA (DNA molecular weight marker) Œ 18 DNA Œ
  19. 19.  DNA ( ‹ ) Automated sequence Œ  Œ gel electropholysis ‹ Œ  By Thanyamon C. 19
  20. 20. (GENOME) (genome) Œ Œ ‹ š gene ‹ ‹ ‹ gene By Thanyamon C. Genome ‹ š  ‹ DNA Œ restriction enzyme Œ Œ gel electropholysis Œ Genome DNA ‹ Restriction fragment length ‹ polymophism (RFLP) DNA ‹ 20 Œ restriction enzyme
  21. 21. (‹) Human Genome Project  š By Thanyamon C.    autosome sex chromosome Human Genome Project ‹ 21 
  22. 22. HUMAN GENOME PROJECT By Thanyamon C. 22
  23. 23.  Œ DNA  Œ   ‹ ‹ HIV By Thanyamon C. ‹ š ( Œ ‹  ) Œ Œ (gene therapy) ‹ SCID š Œ† ‹ Œ Œ Œ Œ  ‹  , HIV, HIV, 23
  24. 24. By Thanyamon C. 24 GENE THERAPY
  25. 25. By Thanyamon C. 25 
  26. 26.  Œ DNA  Œ   By Thanyamon C.    Œ  DNA (DNA fingerprint) DNA (DNA band) š ‹ DNA š  (Œ ‹ ‹ Œ  DNA ‹ ) 26
  27. 27. By Thanyamon C. 27  
  28. 28. By Thanyamon C. 28  
  29. 29. By Thanyamon C. 29 ΠDNA
  30. 30.  Œ DNA ( ‹ )  Œ GMOs (Genetically Modified Organisms) Organisms) By Thanyamon C.  molecular breeding  ( ‹ Œ ) ‚ Š ‹ 30
  31. 31.   Œ ‹   Œ , Œ  By Thanyamon C. (transgenic animal), animal),  (in vitro fertilization) <Œ ‹ Œ> 31
  32. 32. ‹ TRANSGENIC ANIMAL By Thanyamon C. 32
  33. 33. Œ (transgenic plant) ‹ Œ Œ ‹ ‡ BT ‹ Œ Œ Œ  ‹ ‡ , Œ , , , By Thanyamon C. Œ Œ ‹ ‹ Œ Œ Œ ‹ Œ ‹ ‹ Œ ( A) Œ Œ ‹ Œ Œ ‹ Œ Œ Œ ‹ ‹ 33
  34. 34. By Thanyamon C. 34 GMOS ‹
  35. 35.  Œ DNA ( ‹ ) Œ  Œ Œ Œ Œ  (mutant) Œ Œ ‹ By Thanyamon C. ‚ Š ‹ (bioinformation) ŒŒ Œ Œ Œ Œ Œ Œ Œ Œ ‹ Œ  ‹ Œ , Œ , ‹ Œ ‹ Œ Œ Œ 35 Œ Œ ‹
  36. 36.  Œ DNA ( ‹ )  Œ Œ Œ   ‹ ‹ By Thanyamon C.  „œ ‹ ‹ ‹ ‹ 36
  37. 37. DNA Œ  ‹ By Thanyamon C. ‹ Œ Œ Œ ‹   Œ Œ  Œ GMOs Œ ‹ š GMOs Œ Œ ŒŒ genome  Œ ‹ Œ ŒŒ ‹ Œ Œ 37
  38. 38. By Thanyamon C. 38
  39. 39. By Thanyamon C. 39 The End

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