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PAPER
CHROMATOGRAPHY
SYNOPSIS;
▪ What is paper chromatography
▪ Instrumentation of paper chromatography
▪ Diagram of paper chromatography
▪ Types of paper chromatography
▪ Principal of paper chromatography
▪ Procedure of paper chromatography
▪ Techniques of paper chromatography
▪ Advantages of paper chromatography
▪ Disadvantages of paper chromatography
▪ Applications of paper chromatography
▪ Reffrencess of paper chromatography
WHAT IS PAPER CHROMATOGRAPHY?
▪ Paper chromatography(pc) is a type of planarchromatographywhere by
chromatographyprocedures are run on specialized paper.
▪ Paper chromatographyis considered to be the slimplestand most widely
used of the chromatographictechniques because of it’s applicabilityto
isolation, identificationand quantitative determination of organic and
inorganiccompounds.
▪ It was first introducedby German scientist Christian Friedrich schonbein
(1865).
INSTRUMENTATION OF PAPER CHROMATOGRAPHY
▪ Stationaryphase and papers
▪ Paper chromatographymobile phase
▪ Chromatographicchamber
DIAGRAM OF PAPER CHROMATOGRAPHY
TYPES OF PAPER CHROMATOGRAPHY
▪ There are two types of paper
chromatography
▪ paper adsorption chromatography
▪ It’s liquid- solid chromatography
▪ Paper partition chromatography
▪ It’s liquid-liquid chromatography
PRINCIPAL OF PAPER CHROMATOGRAPHY
▪ The principal of separation is mainly partition rather than adsorption.
▪ Substances are distributed between a stationary phase and a mobile phase.
▪ Moisture which acts as a stationary phase.
▪ Organic solvent/buffer are used as a mobile phase.
▪ The developing solution travels up the stationary phase carrying the sample with it.
▪ Components of the sample will separate readily according to how strongly they
adsorb onto the stationary phase versus how readily they dissolve in the mobile
phase
PROCEDURE OF PAPER CHROMATOGRAPHY
▪ Chromatographicpaper of appropriatesize and shape is selected.
▪ A drop of test solution is applied as a small spot on a paper and the spot is
dried.
▪ Mobile phase is kept in chamber.
▪ The paper is placed in the chamber is such way that the lower edge of the
paper is diapped into a solvent (mobile phase)
▪ Solvent will raise through the paper by capillaryaction and it reaches the
spot of the test solution.
▪ Then various sample components are moved the paper is taken out of the
chamber and dried.
▪ Various spots are visualizaedby suitablereagents.
▪ Rf values are calculated for each component of sample.
FROMULA OF PAPER CHROMATOGRAPHY
TECHNIQUES OF PAPER CHROMATOGRAPHY
Ascending chromatography
▪ Ascending chromatography
Descending chromatography
TECHNIQUES OF PAPER CHROMATOGRAPHY
Radial chromatography
ADVANTAGES OF PAPER CHROMATOGRAPHY
▪ Paper chromatographyis a less expensive and easy compared to other
chromatographymethods.
▪ It’s requires very less amount of sample.
▪ Both unknown inorganicas well as compounds can be identified by paper
chromatographymethod.
DISADVANTAGES OF PAPER
CHROMATOGRAPHY
▪ Large quantityof sample cannot be applied on paper chromatographyand it
requires more time for separation.
▪ It is generally used for quantitative analysis
▪ Complex mixtures cannot be separated by paper chromatography.
APPLICATIONS OF PAPER
CHROMATOGRAPHY
▪ Preferred for analysisof polar compounds such as amino acids, sugars,
natural products
▪ Separation and analysis of plant extracts.
▪ Separation of vitamins, proteins and antibiotics.
REFERENCES
▪ Instrumental methods of chemical analysis by Dr.b.k.sharma Retd.principle&Head.
▪ https://www.studocu.com/in/document/jamia-millia-islamia/techniques-in-
biology/paper-chromatography-and-its-principles-instrumentations-applications-
procedure-and-advantages-and-disadvantages/19 604327
▪ https://microbenotes.com/paper-chromatography/
▪ 44095-14-upahyay biophysical chemistry text book.
G.ABINAYA.
I.B.SC.MICROBIOLOGY

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What is paper chromatography, principal, procedure,types, diagram, advantages and disadvantage

  • 2. SYNOPSIS; ▪ What is paper chromatography ▪ Instrumentation of paper chromatography ▪ Diagram of paper chromatography ▪ Types of paper chromatography ▪ Principal of paper chromatography ▪ Procedure of paper chromatography ▪ Techniques of paper chromatography ▪ Advantages of paper chromatography ▪ Disadvantages of paper chromatography ▪ Applications of paper chromatography ▪ Reffrencess of paper chromatography
  • 3. WHAT IS PAPER CHROMATOGRAPHY? ▪ Paper chromatography(pc) is a type of planarchromatographywhere by chromatographyprocedures are run on specialized paper. ▪ Paper chromatographyis considered to be the slimplestand most widely used of the chromatographictechniques because of it’s applicabilityto isolation, identificationand quantitative determination of organic and inorganiccompounds. ▪ It was first introducedby German scientist Christian Friedrich schonbein (1865).
  • 4. INSTRUMENTATION OF PAPER CHROMATOGRAPHY ▪ Stationaryphase and papers ▪ Paper chromatographymobile phase ▪ Chromatographicchamber
  • 5. DIAGRAM OF PAPER CHROMATOGRAPHY
  • 6. TYPES OF PAPER CHROMATOGRAPHY ▪ There are two types of paper chromatography ▪ paper adsorption chromatography ▪ It’s liquid- solid chromatography ▪ Paper partition chromatography ▪ It’s liquid-liquid chromatography
  • 7. PRINCIPAL OF PAPER CHROMATOGRAPHY ▪ The principal of separation is mainly partition rather than adsorption. ▪ Substances are distributed between a stationary phase and a mobile phase. ▪ Moisture which acts as a stationary phase. ▪ Organic solvent/buffer are used as a mobile phase. ▪ The developing solution travels up the stationary phase carrying the sample with it. ▪ Components of the sample will separate readily according to how strongly they adsorb onto the stationary phase versus how readily they dissolve in the mobile phase
  • 8. PROCEDURE OF PAPER CHROMATOGRAPHY ▪ Chromatographicpaper of appropriatesize and shape is selected. ▪ A drop of test solution is applied as a small spot on a paper and the spot is dried. ▪ Mobile phase is kept in chamber. ▪ The paper is placed in the chamber is such way that the lower edge of the paper is diapped into a solvent (mobile phase) ▪ Solvent will raise through the paper by capillaryaction and it reaches the spot of the test solution. ▪ Then various sample components are moved the paper is taken out of the chamber and dried. ▪ Various spots are visualizaedby suitablereagents. ▪ Rf values are calculated for each component of sample.
  • 9. FROMULA OF PAPER CHROMATOGRAPHY
  • 10. TECHNIQUES OF PAPER CHROMATOGRAPHY Ascending chromatography ▪ Ascending chromatography Descending chromatography
  • 11. TECHNIQUES OF PAPER CHROMATOGRAPHY Radial chromatography
  • 12. ADVANTAGES OF PAPER CHROMATOGRAPHY ▪ Paper chromatographyis a less expensive and easy compared to other chromatographymethods. ▪ It’s requires very less amount of sample. ▪ Both unknown inorganicas well as compounds can be identified by paper chromatographymethod.
  • 13. DISADVANTAGES OF PAPER CHROMATOGRAPHY ▪ Large quantityof sample cannot be applied on paper chromatographyand it requires more time for separation. ▪ It is generally used for quantitative analysis ▪ Complex mixtures cannot be separated by paper chromatography.
  • 14. APPLICATIONS OF PAPER CHROMATOGRAPHY ▪ Preferred for analysisof polar compounds such as amino acids, sugars, natural products ▪ Separation and analysis of plant extracts. ▪ Separation of vitamins, proteins and antibiotics.
  • 15. REFERENCES ▪ Instrumental methods of chemical analysis by Dr.b.k.sharma Retd.principle&Head. ▪ https://www.studocu.com/in/document/jamia-millia-islamia/techniques-in- biology/paper-chromatography-and-its-principles-instrumentations-applications- procedure-and-advantages-and-disadvantages/19 604327 ▪ https://microbenotes.com/paper-chromatography/ ▪ 44095-14-upahyay biophysical chemistry text book.