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M.Prasad Naidu
MSc Medical Biochemistry, Ph.D,.
 It is an improvement over mean deviation
 Measure of dispersion
 Used most commonly in statistical analyses
Calculation of SD
 First find the mean of series
 Find the deviation or difference of individual
measurement from mean
 Next find the sum of squares of deviation or
difference of individual measurements from their
mean
 Now find the variance (Var) – mean squared
deviation
var = ∑ (X - X) 2/ n
 If large sample size :
 If sample less than 30 :
Square root of variance that gives SD
For ex :
Mean = 2+5+3+4+1/5 = 15/5 = 3
Var = 10/5 = 2
Uses of SD It summarizes the deviation of a large distribution from
mean in one figure used as unit of variation
 Indicates whether the variation of difference of an
individual from mean is by chance
 Helps in finding the SE which determines whether the
difference between means of two similar samples is by
chance or real
 Helps in finding the suitable size of sample for valid
conclusion.
 The shape of curve will depend upon mean and SD of
which in turn depend upon the number and nature of
observation.
 In normal curve :-
 Area b/w 1 SD on either side of mean will include
approximately 68% of values in distribution
 Area b/w 2 SD is 95%
 Area b/w 3 SD is 99.7%
 These limits on either side of mean are called
“confidence limits”
SD of normal curve
Standard normal curve
 Smooth
 bell shaped
 Perfectly symmetrical
 Based on infinity large number of observation
 Total area of curve = 1
 Mean = 0
 SD = 1
 Mean , median and mode all coincide
SD of normal curve Bell shaped curve will show an inflexion on the
ascending as well as descending units of curve
 If vertical lines are drawn from each of these points they
will intersect the X axis on either side of the mean at an
equal distance from it
 A large portion of area under the normal curve has been
included in portion of curve b/w the 2 points of
inflexion
 The distance b/w the mean and point of inflexion
either side is equal to SD and is denoted by a + sign
prefixed to it to indicate that it extends on either
side of mean.
 If another vertical line is drawn an either side of
mean at a distance equal to twice SD most of
values in distribution table would have been
included in this part of curve
 In most cases, + SD will include 2/3 of sample
values and mean + 2 SD will include 90% of
values.
Standard deviation
Standard deviation