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Sperm Count Using Neubauer Chamber | Semen Analysis Procedure, Calculation & Interpretation

This presentation provides a step-by-step explanation of sperm counting using the Improved Neubauer Chamber, one of the most important practical procedures in semen analysis. It is designed for Medical Laboratory Technology students and healthcare professionals.

Health & Medicineā—¦
Related topics:
Male Infertility Insightsā€¢Laboratory Technologyā€¢
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Sperm Count Requirements, Procedure & Calculation Clinical Pathology & Serology
What is Sperm Count? Normal sperm count according to WHO is ā‰„15 million sperm/mL. It is performed to: ā€¢ Assess male fertility ā€¢ Evaluate sperm concentration ā€¢ Diagnose infertility
Requirements Sterile container Avoid lubricants Deliver within 1 hour Keep the sample close to body temperature during transport
Laboratory Requirements Equipment Required Microscope Neubauer Chamber Diluting Fluid Pipette Glass Slides Cover Slip
Patient should: ā€¢ Wash hands ā€¢ Collect semen in sterile container ā€¢ Avoid contamination ā€¢ Transport quickly Collection of Semen Sample Collection
Initial Examination Observe: Volume Color Viscosity Normal Findings Volume: 1.5ā€“5 mL Color: Whitish Gray
Normal semen becomes liquid in 20ā€“30 minutes If not liquefied ā†“ May indicate Prostate gland problem Liquefaction Deficiency of prostatic enzymes.
Dilution Purpose: Reduce sperm concentration Prevent clumping Easy counting
ā€¢ Place diluted sample ā€¢ Apply cover slip ā€¢ Allow sperm to settle for 2ā€“3 minutes Then examine under microscope . Loading Neubauer Chamber
Important Terms
Factors Affecting Sperm Count Medical Factors Varicocele Infection Hormonal imbalance Lifestyle Factors Smoking Alcohol Drugs Heat exposure