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BY NICOLE MICHEL ROJAS TORRES
3RD SEMESTER
UNIVERSITY PONTIFICIA BOLIVARIAN
STAPHYLOCOCCUS AUREUS
Transported via contaminated apparatus or
through directly contaminated persons more
often result
in nosocomial infections.
Gram-positive bacterium Facultative anaerobe
Methicillin resistant
Enzyme produced by bacteriophages (viruses
that infect bacteria) to degrade the bacterial cell
wall during the viral replication cycle, ultimately
leading to the release of newly formed viral
particles.
ENDOLYSIN
Enzyme
“endolysin”
Specificity
target
Combined effects
Analyze Staphylococcus aureus
Bacteriophage 52 Endolysin Exhibits Anti-
Biofilm and Broad Antibacterial Activity
Against Gram-Positive
Bacteria
PURPOSE
METODOLOGY
PCR
Amplifies a specific sequence of genetic
material in a sample
The presence of endolysin is evaluated in
five sequences of Staphylococcus Aereus
SEQUENCING
Procedure used to determine the
exact sequence of nucleotides in a
specific segment of DNA
corresponding to the Endolysin gen
METODOLOGY
PURIFICATION
By purifying Endolysin, we
sought to obtain a highly
concentrated sample and
maximize the expression of this.
LYTIC ACTIVITY
Was determined
as the presence of bacterial lysis and
considered to indicate positive endolysin
activity.
RESULTS
RESULTS
DISCUSION
Author What say? Article
Gutierrez D, Fernandez L,
Rodriguez A, Garcia P
(2018)
Lu Y, Wang Y, Wang J, Zhao Y,
Zhong Q, Li G, Fu Z, Lu S
(2021)
Gondil VS, Harjai K,
Chhibber S (2020)
“Are Phage Lytic Proteins the
Secret Weapon To Kill
Staphylococcus aureus?”
“Phage endolysin LysP108 showed
Promising Antibacte-
rial potential against Methicillin-
resistant Staphylococcus aureus”
“Endolysins as emerging alternative
therapeutic agents to counter drug-
resistant infections”
Introducing phage lytic proteins as
human therapeutics against S.
aureus infections.
The endolysin in combination with
antibiotics can avoid the development
of resistance in target cells
Endolysins have been studied against
a number of drug-resistant pathogens
to assess their therapeutic ability.
CONCLUSION
The investigation support the
Advancing Medical Knowledge
and Understanding to recognized
and provide more accurate
diagnoses also improve overall
patient outcomes
Innovation and Development of
New Therapies
approaches offer potential
solutions for previously
untreatable conditions and
provide hope for patients
Seminario Biologia Molecular Nicole Michel Rojas Torres

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Seminario Biologia Molecular Nicole Michel Rojas Torres

  • 1. BY NICOLE MICHEL ROJAS TORRES 3RD SEMESTER UNIVERSITY PONTIFICIA BOLIVARIAN
  • 2. STAPHYLOCOCCUS AUREUS Transported via contaminated apparatus or through directly contaminated persons more often result in nosocomial infections. Gram-positive bacterium Facultative anaerobe Methicillin resistant
  • 3. Enzyme produced by bacteriophages (viruses that infect bacteria) to degrade the bacterial cell wall during the viral replication cycle, ultimately leading to the release of newly formed viral particles. ENDOLYSIN Enzyme “endolysin” Specificity target Combined effects
  • 4. Analyze Staphylococcus aureus Bacteriophage 52 Endolysin Exhibits Anti- Biofilm and Broad Antibacterial Activity Against Gram-Positive Bacteria PURPOSE
  • 5. METODOLOGY PCR Amplifies a specific sequence of genetic material in a sample The presence of endolysin is evaluated in five sequences of Staphylococcus Aereus SEQUENCING Procedure used to determine the exact sequence of nucleotides in a specific segment of DNA corresponding to the Endolysin gen
  • 6. METODOLOGY PURIFICATION By purifying Endolysin, we sought to obtain a highly concentrated sample and maximize the expression of this. LYTIC ACTIVITY Was determined as the presence of bacterial lysis and considered to indicate positive endolysin activity.
  • 9. DISCUSION Author What say? Article Gutierrez D, Fernandez L, Rodriguez A, Garcia P (2018) Lu Y, Wang Y, Wang J, Zhao Y, Zhong Q, Li G, Fu Z, Lu S (2021) Gondil VS, Harjai K, Chhibber S (2020) “Are Phage Lytic Proteins the Secret Weapon To Kill Staphylococcus aureus?” “Phage endolysin LysP108 showed Promising Antibacte- rial potential against Methicillin- resistant Staphylococcus aureus” “Endolysins as emerging alternative therapeutic agents to counter drug- resistant infections” Introducing phage lytic proteins as human therapeutics against S. aureus infections. The endolysin in combination with antibiotics can avoid the development of resistance in target cells Endolysins have been studied against a number of drug-resistant pathogens to assess their therapeutic ability.
  • 10. CONCLUSION The investigation support the Advancing Medical Knowledge and Understanding to recognized and provide more accurate diagnoses also improve overall patient outcomes Innovation and Development of New Therapies approaches offer potential solutions for previously untreatable conditions and provide hope for patients