SlideShare a Scribd company logo
1 of 11
Download to read offline
Lina Marcela Charris Ramírez
Medicina
III Semestre
Universidad Pontificia Bolivariana
Campylobacter coli
Campylobacter coli is one of the most prevalent organisms and a major
cause of human acute enteritis,
Poultry meat
It is considered that the main source of infections by
this disease is due to the ingestion of contaminated
poultry meat.
Determine the genotypic diversity of Campylobacter coli strains present in
poultry meat samples using PFGE, Sau-PCR and fa-DGGE techniques to
compare their effectiveness, their discriminatory capacity and their
potential to be used in primary laboratories.
General objective
Métodos
Identificación de Campylobacter coli
En el estudio las muestras fueron tomadas e
incubadas en las condiciones adecuadas para poder
analizarlas.
Análisis PFGE
En este método, se corta el ADN de las bacterias con
enzimas de restricción, generando fragmentos de
diferentes tamaños.
Métodos
Análisis de Fla-DGGE
Análisis Sau-PCR
Método de reacción en cadena de la polimerasa
(PCR) que utiliza la enzimas para digerir el ADN
en sitios específicos.
Método utilizado para examinar la diversidad
genética de secuencias de genes específicos
relacionados con genes de flagelina
Resultados
Resultados
Kong N, Luo Y, Liu J
¨Subtyping of Campylobacter coli isolated from raw
poultry meat in retail markets using amplified
intergenic locus polymorphism - A novel rapid
subtyping method¨
What did he say?
Does it agree with the text?
No significant differences
were found in the prevalence
of campylobacter coli
between chicken and duck
samples.
Optimal typing methods need
to have appropriate
discriminatory power and
must be assessed for the
turnaround time, throughput,
cost, and technical difficulty
of the typing method
Yes, the same conclusion
was reached in the text
Yes, the text reaches the
same conclusion.
Author
Clark CG, Taboada E, Grant
CCR
¨Comparison of Molecular Typing Methods Useful
for Detecting Clusters of Campylobacter jejuni and C.
coli Isolates through Routine Surveillance
¨
Rosner BM, Schielke A,
Didelot X
¨A combined case-control and molecular source
attribution study of human Campylobacter infections
in Germany, 2011–2014”¨
The contaminated poultry
products may lead the
Campylobacter outbreaks
Yes, the study reached
the same conclusion.
Discussion
Research in medical and molecular biology fields is fundamental
for advancing the understanding of diseases, identifying
pathogens, and developing effective treatments. .
Research in molecular biology in these fields not only expands our
scientific knowledge but also has a direct impact on public health
and global health decision-making.
Conclusions
Seminario biología molecular Lina Charris

More Related Content

Similar to Seminario biología molecular Lina Charris

CHARACTERIZATION OF MYCOPLASMA GALLISEPTICUM IN POULTRY
CHARACTERIZATION OF MYCOPLASMA GALLISEPTICUM IN POULTRYCHARACTERIZATION OF MYCOPLASMA GALLISEPTICUM IN POULTRY
CHARACTERIZATION OF MYCOPLASMA GALLISEPTICUM IN POULTRYMuniruzzaman
 
Microbiological Culture sensitivity tests
Microbiological Culture sensitivity testsMicrobiological Culture sensitivity tests
Microbiological Culture sensitivity testsDr Vimali
 
Univ bacteriav PCR Primer
Univ bacteriav PCR PrimerUniv bacteriav PCR Primer
Univ bacteriav PCR PrimerZulkifli As
 
Resume Jean Wei 111616
Resume Jean Wei 111616Resume Jean Wei 111616
Resume Jean Wei 111616Jean Wei
 
1 s2.0-s0015028214018603-main
1 s2.0-s0015028214018603-main1 s2.0-s0015028214018603-main
1 s2.0-s0015028214018603-main鋒博 蔡
 
Epidemiology and molecular typing of klebsiella pneumonia with the extended s...
Epidemiology and molecular typing of klebsiella pneumonia with the extended s...Epidemiology and molecular typing of klebsiella pneumonia with the extended s...
Epidemiology and molecular typing of klebsiella pneumonia with the extended s...BioMedSciDirect Publications
 
Molecular Detection of Epstein-Barr Virus and Human Cytomegalovirus Antigen E...
Molecular Detection of Epstein-Barr Virus and Human Cytomegalovirus Antigen E...Molecular Detection of Epstein-Barr Virus and Human Cytomegalovirus Antigen E...
Molecular Detection of Epstein-Barr Virus and Human Cytomegalovirus Antigen E...CrimsonpublishersCancer
 
Newer investigations of tuberculosis
Newer investigations of tuberculosisNewer investigations of tuberculosis
Newer investigations of tuberculosisVishnu Ambareesh
 
Detection of the Salmonella Typhi bacteria in contaminated egg using Real Tim...
Detection of the Salmonella Typhi bacteria in contaminated egg using Real Tim...Detection of the Salmonella Typhi bacteria in contaminated egg using Real Tim...
Detection of the Salmonella Typhi bacteria in contaminated egg using Real Tim...Maleja29
 
Development and Validation of a Two-Site Immunoradiometric assay for Glypican...
Development and Validation of a Two-Site Immunoradiometric assay for Glypican...Development and Validation of a Two-Site Immunoradiometric assay for Glypican...
Development and Validation of a Two-Site Immunoradiometric assay for Glypican...Premier Publishers
 
Development of novel visual detection methodology for Salmonella in meat usin...
Development of novel visual detection methodology for Salmonella in meat usin...Development of novel visual detection methodology for Salmonella in meat usin...
Development of novel visual detection methodology for Salmonella in meat usin...MariaAngelicaArangoP
 
Tracking Immune Biomarkers and the Human Gut Microbiome: Inflammation, Croh...
Tracking Immune Biomarkers and the Human Gut Microbiome: Inflammation, Croh...Tracking Immune Biomarkers and the Human Gut Microbiome: Inflammation, Croh...
Tracking Immune Biomarkers and the Human Gut Microbiome: Inflammation, Croh...Larry Smarr
 

Similar to Seminario biología molecular Lina Charris (20)

Genetica bioinformatica
Genetica bioinformaticaGenetica bioinformatica
Genetica bioinformatica
 
Raju Paper
Raju PaperRaju Paper
Raju Paper
 
48_7
48_748_7
48_7
 
CHARACTERIZATION OF MYCOPLASMA GALLISEPTICUM IN POULTRY
CHARACTERIZATION OF MYCOPLASMA GALLISEPTICUM IN POULTRYCHARACTERIZATION OF MYCOPLASMA GALLISEPTICUM IN POULTRY
CHARACTERIZATION OF MYCOPLASMA GALLISEPTICUM IN POULTRY
 
Microbiological Culture sensitivity tests
Microbiological Culture sensitivity testsMicrobiological Culture sensitivity tests
Microbiological Culture sensitivity tests
 
Antibody validation
Antibody validationAntibody validation
Antibody validation
 
Univ bacteriav PCR Primer
Univ bacteriav PCR PrimerUniv bacteriav PCR Primer
Univ bacteriav PCR Primer
 
Resume Jean Wei 111616
Resume Jean Wei 111616Resume Jean Wei 111616
Resume Jean Wei 111616
 
1 s2.0-s0015028214018603-main
1 s2.0-s0015028214018603-main1 s2.0-s0015028214018603-main
1 s2.0-s0015028214018603-main
 
Rloia Pickettiir
Rloia PickettiirRloia Pickettiir
Rloia Pickettiir
 
Histopathological and RT-PCR Detection of Mycobacterium paratuberculosis in T...
Histopathological and RT-PCR Detection of Mycobacterium paratuberculosis in T...Histopathological and RT-PCR Detection of Mycobacterium paratuberculosis in T...
Histopathological and RT-PCR Detection of Mycobacterium paratuberculosis in T...
 
Epidemiology and molecular typing of klebsiella pneumonia with the extended s...
Epidemiology and molecular typing of klebsiella pneumonia with the extended s...Epidemiology and molecular typing of klebsiella pneumonia with the extended s...
Epidemiology and molecular typing of klebsiella pneumonia with the extended s...
 
NDBD
NDBDNDBD
NDBD
 
Molecular Detection of Epstein-Barr Virus and Human Cytomegalovirus Antigen E...
Molecular Detection of Epstein-Barr Virus and Human Cytomegalovirus Antigen E...Molecular Detection of Epstein-Barr Virus and Human Cytomegalovirus Antigen E...
Molecular Detection of Epstein-Barr Virus and Human Cytomegalovirus Antigen E...
 
Techniques of diagnostic Mycology- 5 hours
Techniques of diagnostic Mycology- 5 hoursTechniques of diagnostic Mycology- 5 hours
Techniques of diagnostic Mycology- 5 hours
 
Newer investigations of tuberculosis
Newer investigations of tuberculosisNewer investigations of tuberculosis
Newer investigations of tuberculosis
 
Detection of the Salmonella Typhi bacteria in contaminated egg using Real Tim...
Detection of the Salmonella Typhi bacteria in contaminated egg using Real Tim...Detection of the Salmonella Typhi bacteria in contaminated egg using Real Tim...
Detection of the Salmonella Typhi bacteria in contaminated egg using Real Tim...
 
Development and Validation of a Two-Site Immunoradiometric assay for Glypican...
Development and Validation of a Two-Site Immunoradiometric assay for Glypican...Development and Validation of a Two-Site Immunoradiometric assay for Glypican...
Development and Validation of a Two-Site Immunoradiometric assay for Glypican...
 
Development of novel visual detection methodology for Salmonella in meat usin...
Development of novel visual detection methodology for Salmonella in meat usin...Development of novel visual detection methodology for Salmonella in meat usin...
Development of novel visual detection methodology for Salmonella in meat usin...
 
Tracking Immune Biomarkers and the Human Gut Microbiome: Inflammation, Croh...
Tracking Immune Biomarkers and the Human Gut Microbiome: Inflammation, Croh...Tracking Immune Biomarkers and the Human Gut Microbiome: Inflammation, Croh...
Tracking Immune Biomarkers and the Human Gut Microbiome: Inflammation, Croh...
 

Recently uploaded

Thermodynamics ,types of system,formulae ,gibbs free energy .pptx
Thermodynamics ,types of system,formulae ,gibbs free energy .pptxThermodynamics ,types of system,formulae ,gibbs free energy .pptx
Thermodynamics ,types of system,formulae ,gibbs free energy .pptxuniversity
 
(9818099198) Call Girls In Noida Sector 14 (NOIDA ESCORTS)
(9818099198) Call Girls In Noida Sector 14 (NOIDA ESCORTS)(9818099198) Call Girls In Noida Sector 14 (NOIDA ESCORTS)
(9818099198) Call Girls In Noida Sector 14 (NOIDA ESCORTS)riyaescorts54
 
User Guide: Capricorn FLX™ Weather Station
User Guide: Capricorn FLX™ Weather StationUser Guide: Capricorn FLX™ Weather Station
User Guide: Capricorn FLX™ Weather StationColumbia Weather Systems
 
bonjourmadame.tumblr.com bhaskar's girls
bonjourmadame.tumblr.com bhaskar's girlsbonjourmadame.tumblr.com bhaskar's girls
bonjourmadame.tumblr.com bhaskar's girlshansessene
 
Pests of Bengal gram_Identification_Dr.UPR.pdf
Pests of Bengal gram_Identification_Dr.UPR.pdfPests of Bengal gram_Identification_Dr.UPR.pdf
Pests of Bengal gram_Identification_Dr.UPR.pdfPirithiRaju
 
Organic farming with special reference to vermiculture
Organic farming with special reference to vermicultureOrganic farming with special reference to vermiculture
Organic farming with special reference to vermicultureTakeleZike1
 
REVISTA DE BIOLOGIA E CIÊNCIAS DA TERRA ISSN 1519-5228 - Artigo_Bioterra_V24_...
REVISTA DE BIOLOGIA E CIÊNCIAS DA TERRA ISSN 1519-5228 - Artigo_Bioterra_V24_...REVISTA DE BIOLOGIA E CIÊNCIAS DA TERRA ISSN 1519-5228 - Artigo_Bioterra_V24_...
REVISTA DE BIOLOGIA E CIÊNCIAS DA TERRA ISSN 1519-5228 - Artigo_Bioterra_V24_...Universidade Federal de Sergipe - UFS
 
GENERAL PHYSICS 2 REFRACTION OF LIGHT SENIOR HIGH SCHOOL GENPHYS2.pptx
GENERAL PHYSICS 2 REFRACTION OF LIGHT SENIOR HIGH SCHOOL GENPHYS2.pptxGENERAL PHYSICS 2 REFRACTION OF LIGHT SENIOR HIGH SCHOOL GENPHYS2.pptx
GENERAL PHYSICS 2 REFRACTION OF LIGHT SENIOR HIGH SCHOOL GENPHYS2.pptxRitchAndruAgustin
 
Pests of jatropha_Bionomics_identification_Dr.UPR.pdf
Pests of jatropha_Bionomics_identification_Dr.UPR.pdfPests of jatropha_Bionomics_identification_Dr.UPR.pdf
Pests of jatropha_Bionomics_identification_Dr.UPR.pdfPirithiRaju
 
User Guide: Pulsar™ Weather Station (Columbia Weather Systems)
User Guide: Pulsar™ Weather Station (Columbia Weather Systems)User Guide: Pulsar™ Weather Station (Columbia Weather Systems)
User Guide: Pulsar™ Weather Station (Columbia Weather Systems)Columbia Weather Systems
 
Call Girls in Majnu Ka Tilla Delhi 🔝9711014705🔝 Genuine
Call Girls in Majnu Ka Tilla Delhi 🔝9711014705🔝 GenuineCall Girls in Majnu Ka Tilla Delhi 🔝9711014705🔝 Genuine
Call Girls in Majnu Ka Tilla Delhi 🔝9711014705🔝 Genuinethapagita
 
Manassas R - Parkside Middle School 🌎🏫
Manassas R - Parkside Middle School 🌎🏫Manassas R - Parkside Middle School 🌎🏫
Manassas R - Parkside Middle School 🌎🏫qfactory1
 
Gas-ExchangeS-in-Plants-and-Animals.pptx
Gas-ExchangeS-in-Plants-and-Animals.pptxGas-ExchangeS-in-Plants-and-Animals.pptx
Gas-ExchangeS-in-Plants-and-Animals.pptxGiovaniTrinidad
 
trihybrid cross , test cross chi squares
trihybrid cross , test cross chi squarestrihybrid cross , test cross chi squares
trihybrid cross , test cross chi squaresusmanzain586
 
CHROMATOGRAPHY PALLAVI RAWAT.pptx
CHROMATOGRAPHY  PALLAVI RAWAT.pptxCHROMATOGRAPHY  PALLAVI RAWAT.pptx
CHROMATOGRAPHY PALLAVI RAWAT.pptxpallavirawat456
 
Oxo-Acids of Halogens and their Salts.pptx
Oxo-Acids of Halogens and their Salts.pptxOxo-Acids of Halogens and their Salts.pptx
Oxo-Acids of Halogens and their Salts.pptxfarhanvvdk
 
Pests of soyabean_Binomics_IdentificationDr.UPR.pdf
Pests of soyabean_Binomics_IdentificationDr.UPR.pdfPests of soyabean_Binomics_IdentificationDr.UPR.pdf
Pests of soyabean_Binomics_IdentificationDr.UPR.pdfPirithiRaju
 
OECD bibliometric indicators: Selected highlights, April 2024
OECD bibliometric indicators: Selected highlights, April 2024OECD bibliometric indicators: Selected highlights, April 2024
OECD bibliometric indicators: Selected highlights, April 2024innovationoecd
 

Recently uploaded (20)

Thermodynamics ,types of system,formulae ,gibbs free energy .pptx
Thermodynamics ,types of system,formulae ,gibbs free energy .pptxThermodynamics ,types of system,formulae ,gibbs free energy .pptx
Thermodynamics ,types of system,formulae ,gibbs free energy .pptx
 
(9818099198) Call Girls In Noida Sector 14 (NOIDA ESCORTS)
(9818099198) Call Girls In Noida Sector 14 (NOIDA ESCORTS)(9818099198) Call Girls In Noida Sector 14 (NOIDA ESCORTS)
(9818099198) Call Girls In Noida Sector 14 (NOIDA ESCORTS)
 
User Guide: Capricorn FLX™ Weather Station
User Guide: Capricorn FLX™ Weather StationUser Guide: Capricorn FLX™ Weather Station
User Guide: Capricorn FLX™ Weather Station
 
bonjourmadame.tumblr.com bhaskar's girls
bonjourmadame.tumblr.com bhaskar's girlsbonjourmadame.tumblr.com bhaskar's girls
bonjourmadame.tumblr.com bhaskar's girls
 
Pests of Bengal gram_Identification_Dr.UPR.pdf
Pests of Bengal gram_Identification_Dr.UPR.pdfPests of Bengal gram_Identification_Dr.UPR.pdf
Pests of Bengal gram_Identification_Dr.UPR.pdf
 
Organic farming with special reference to vermiculture
Organic farming with special reference to vermicultureOrganic farming with special reference to vermiculture
Organic farming with special reference to vermiculture
 
REVISTA DE BIOLOGIA E CIÊNCIAS DA TERRA ISSN 1519-5228 - Artigo_Bioterra_V24_...
REVISTA DE BIOLOGIA E CIÊNCIAS DA TERRA ISSN 1519-5228 - Artigo_Bioterra_V24_...REVISTA DE BIOLOGIA E CIÊNCIAS DA TERRA ISSN 1519-5228 - Artigo_Bioterra_V24_...
REVISTA DE BIOLOGIA E CIÊNCIAS DA TERRA ISSN 1519-5228 - Artigo_Bioterra_V24_...
 
GENERAL PHYSICS 2 REFRACTION OF LIGHT SENIOR HIGH SCHOOL GENPHYS2.pptx
GENERAL PHYSICS 2 REFRACTION OF LIGHT SENIOR HIGH SCHOOL GENPHYS2.pptxGENERAL PHYSICS 2 REFRACTION OF LIGHT SENIOR HIGH SCHOOL GENPHYS2.pptx
GENERAL PHYSICS 2 REFRACTION OF LIGHT SENIOR HIGH SCHOOL GENPHYS2.pptx
 
Let’s Say Someone Did Drop the Bomb. Then What?
Let’s Say Someone Did Drop the Bomb. Then What?Let’s Say Someone Did Drop the Bomb. Then What?
Let’s Say Someone Did Drop the Bomb. Then What?
 
Pests of jatropha_Bionomics_identification_Dr.UPR.pdf
Pests of jatropha_Bionomics_identification_Dr.UPR.pdfPests of jatropha_Bionomics_identification_Dr.UPR.pdf
Pests of jatropha_Bionomics_identification_Dr.UPR.pdf
 
User Guide: Pulsar™ Weather Station (Columbia Weather Systems)
User Guide: Pulsar™ Weather Station (Columbia Weather Systems)User Guide: Pulsar™ Weather Station (Columbia Weather Systems)
User Guide: Pulsar™ Weather Station (Columbia Weather Systems)
 
PLASMODIUM. PPTX
PLASMODIUM. PPTXPLASMODIUM. PPTX
PLASMODIUM. PPTX
 
Call Girls in Majnu Ka Tilla Delhi 🔝9711014705🔝 Genuine
Call Girls in Majnu Ka Tilla Delhi 🔝9711014705🔝 GenuineCall Girls in Majnu Ka Tilla Delhi 🔝9711014705🔝 Genuine
Call Girls in Majnu Ka Tilla Delhi 🔝9711014705🔝 Genuine
 
Manassas R - Parkside Middle School 🌎🏫
Manassas R - Parkside Middle School 🌎🏫Manassas R - Parkside Middle School 🌎🏫
Manassas R - Parkside Middle School 🌎🏫
 
Gas-ExchangeS-in-Plants-and-Animals.pptx
Gas-ExchangeS-in-Plants-and-Animals.pptxGas-ExchangeS-in-Plants-and-Animals.pptx
Gas-ExchangeS-in-Plants-and-Animals.pptx
 
trihybrid cross , test cross chi squares
trihybrid cross , test cross chi squarestrihybrid cross , test cross chi squares
trihybrid cross , test cross chi squares
 
CHROMATOGRAPHY PALLAVI RAWAT.pptx
CHROMATOGRAPHY  PALLAVI RAWAT.pptxCHROMATOGRAPHY  PALLAVI RAWAT.pptx
CHROMATOGRAPHY PALLAVI RAWAT.pptx
 
Oxo-Acids of Halogens and their Salts.pptx
Oxo-Acids of Halogens and their Salts.pptxOxo-Acids of Halogens and their Salts.pptx
Oxo-Acids of Halogens and their Salts.pptx
 
Pests of soyabean_Binomics_IdentificationDr.UPR.pdf
Pests of soyabean_Binomics_IdentificationDr.UPR.pdfPests of soyabean_Binomics_IdentificationDr.UPR.pdf
Pests of soyabean_Binomics_IdentificationDr.UPR.pdf
 
OECD bibliometric indicators: Selected highlights, April 2024
OECD bibliometric indicators: Selected highlights, April 2024OECD bibliometric indicators: Selected highlights, April 2024
OECD bibliometric indicators: Selected highlights, April 2024
 

Seminario biología molecular Lina Charris

  • 1. Lina Marcela Charris Ramírez Medicina III Semestre Universidad Pontificia Bolivariana
  • 2. Campylobacter coli Campylobacter coli is one of the most prevalent organisms and a major cause of human acute enteritis,
  • 3. Poultry meat It is considered that the main source of infections by this disease is due to the ingestion of contaminated poultry meat.
  • 4. Determine the genotypic diversity of Campylobacter coli strains present in poultry meat samples using PFGE, Sau-PCR and fa-DGGE techniques to compare their effectiveness, their discriminatory capacity and their potential to be used in primary laboratories. General objective
  • 5. Métodos Identificación de Campylobacter coli En el estudio las muestras fueron tomadas e incubadas en las condiciones adecuadas para poder analizarlas. Análisis PFGE En este método, se corta el ADN de las bacterias con enzimas de restricción, generando fragmentos de diferentes tamaños.
  • 6. Métodos Análisis de Fla-DGGE Análisis Sau-PCR Método de reacción en cadena de la polimerasa (PCR) que utiliza la enzimas para digerir el ADN en sitios específicos. Método utilizado para examinar la diversidad genética de secuencias de genes específicos relacionados con genes de flagelina
  • 9. Kong N, Luo Y, Liu J ¨Subtyping of Campylobacter coli isolated from raw poultry meat in retail markets using amplified intergenic locus polymorphism - A novel rapid subtyping method¨ What did he say? Does it agree with the text? No significant differences were found in the prevalence of campylobacter coli between chicken and duck samples. Optimal typing methods need to have appropriate discriminatory power and must be assessed for the turnaround time, throughput, cost, and technical difficulty of the typing method Yes, the same conclusion was reached in the text Yes, the text reaches the same conclusion. Author Clark CG, Taboada E, Grant CCR ¨Comparison of Molecular Typing Methods Useful for Detecting Clusters of Campylobacter jejuni and C. coli Isolates through Routine Surveillance ¨ Rosner BM, Schielke A, Didelot X ¨A combined case-control and molecular source attribution study of human Campylobacter infections in Germany, 2011–2014”¨ The contaminated poultry products may lead the Campylobacter outbreaks Yes, the study reached the same conclusion. Discussion
  • 10. Research in medical and molecular biology fields is fundamental for advancing the understanding of diseases, identifying pathogens, and developing effective treatments. . Research in molecular biology in these fields not only expands our scientific knowledge but also has a direct impact on public health and global health decision-making. Conclusions