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Determination of Sample Size

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- 1. Sample Size Estimation Mr Sathish Rajamani Associate Professor, Ved Nursing College, Panipat Introduction Quantitative researchers need to pay attention to the number of participants needed to achieve statistical conclusion validity. A procedure called power analysis (Cohen, 1988) cab be used to estimate sample size needs. Sampling Fundamentals Universe / Population: ‘Universe’ refers to the total of the items or units in any field of inquiry, whereas the term ‘population’ refers to the total of items about which information is desired. Statistic(s) and parameter(s): A statistic is a characteristic of a sample, whereas a parameter is a characteristic of a population. Sampling Error: The error that arises as a result of taking a sample from a population rather than using the whole population is known as sampling error. Confidence Interval: the range of values within which a population parameter is estimated to lie, at a specified probability. (e.g., 95% CI). Sampling Distribution: A sampling distribution is a statistic obtained through a large number of samples drawn from a specific population. Sample Size: This is the sub-population to be studied in order to make an inference to a reference population. What is Sample Size Determination? Sample size determination is the mathematical estimation of the number of subjects / units to be included in a study. Why to Determine the Sample Size in a Study? To allow for appropriate analysis To provide the desired level of accuracy To allow validity of significance test Attributes of a Good Sample To extrapolate the inference of the sample to the population, the sample should be.
- 2. A representative of the population Should be a large enough If a sample is too large Good Precision Less Errors and Less Bias But Waste of money, time and resources Not Cost-effective If a sample is too small Inaccurate results More source of bias Power of the study comes down Study fails to give the meaningful information Waste of resources on a inaccurate study Methods to Calculate Sample Size There are four methods used in estimating the sample size. 1. Use of formulae 2. Ready Made Table 3. Nomograms 4. Computer Software Use of Formula’s 1. Calculating Proportion This is used in cases when we are trying to find proportions. E.g. for studies like 1. Estimation of prevalence of tuberculosis in Panipat city 2018 2. Prevalence of extra pyramid side - effects as a complication of anti-psychotic drugs. N = 4PQ/d2 Where, P = Prevalence from Previous Studies Q = 100 – P d = allowable error ( 5 – 20% of P)
- 3. 2. Calculating difference in proportion This is used when we measure the significance of difference between two proportions. E.g. for studies like 1. Diagnostic supremacy of CT chest V/s X-ray chest in tuberculosis patients 2. Success rate of streptomycin V/s kanamycin in cure of MDR – TB N = 15.7 x ꝑ x Q (P1 – P2)2 Where P1 and P2 are the proportions of the two groups. ꝑ is the average of P1 and P2 100- ꝑ 3. Calculating the Mean This formula is used in quantitative studies where we are estimating the mean of the study group. E.g. for studies like Estimation of mean age of diagnosis of tuberculosis in Panipat Study N = 4σ2 / d2 Where, σ (Sigma) is the standard deviation as in similar studies done previously d = allowable error (5 – 20% of σ) 4. Calculating difference in Means This is used in studies where we are calculating the difference achieved quantitatively during the study. E.g. for studies like Average weight gain in patients of tuberculosis before and after DOTS programme N = 15.7 (σ1 x σ2) / d 2 Where, σ1and σ2 are the standard deviations of the 2 study groups, d is the smallest meaningful difference that can be measured. In before – after type of studies, σ1= σ2 = σ
- 4. Use of readymade Table for Sample Size Calculation Sample size determination table are available in readymade to estimate the size of the sample in a study. Use of Nomogram for Sample Size Calculation Altman’s nomogram is a very clever graphical method for calculating sample sizes. Use of Computer Software for Sample Size Calculation and Power Analysis The following software can be used to calculate sample size and Power. Epi – Info nQuerry Power & Precision SPSS STATA Conclusion Sample size determination is one of the most essential components of every research. The larger the sample size, the higher the degree of accuracy, but this is limit by the availability of resources. ************ Thank You *************