SlideShare a Scribd company logo
Role of WGS in microbial biopesticides
safety assessment
Sara Lamperti
Global Regulatory Toxicologist
Corteva Agriscience
Crop Life International February 26, 2024
Whole genome sequencing (WGS)
What is it?
Whole genome sequencing (WGS) is a process to
determine the complete DNA sequence of an organism's
genome, using high-throughput sequencing technologies.
It provides a comprehensive view of an organism's
genetic makeup.
Methods:
Next-generation sequencing (NGS): short reads
(most common)
Single-Molecule Real-Time (SMRT) sequencing: Long
reads (better choice)
Nanopore sequencing: Long reads and portable
2
Limits:
availability of correctly annotated
sequence information
Advantages:
• to help the evaluation/registrability
of the microorganisms
• to reduce the animal testing
Whole genome sequence (WGS)
Scope :
3
➢ Identify microorganism at strain level
➢ To confirm the absence of known genes involved in pathogenicity (cluster toxins)
➢ Phylogenetic tree to show the strain is phylogenetically away from pathogenic strains
➢ Identification antimicrobial resistance (AMR) genes
➢ Explore genome to predict secondary metabolites or exclude the metabolites of
potential concern
Example of evaluation of the potential production of toxin X by a
fungus strain
4
Query
Target Strain
Similarity (%)
Toxigenic strain*
Similarity (%)
Gene 1 99.59 99.34
Gene 2 34.58 99.80
Gene 3 35.47 99.48
Gene 4 42.17 98.13
Gene 5 29.84 98.56
Gene 6 38.85 97.40
Gene 7 39.17 97.57
Gene 8 53.15 100
Gene 9 37.60 99.62
Gene 10 41.28 99.68
Gene 11 42.93 99.94
Gene 12 39.92 99.95
Gene 13 39.52 78.91
* is a known toxin X producing strain used as a positive control
Conclusion: only 1 of 13 toxin X production pathway genes was found in target strain’s genome with high
similarity. In contrast, all 13 genes were found in the toxigenic strain with high similarity. The target strain is
very unlikely can produce toxin X
Blast toxin X biosynthetic gene clusters against target strain and known toxigenic strain’s genomes
5
*
Special Thank You To
Corteva Colleague
Xiao (Eric) Huang

More Related Content

Similar to Role of genome sequencing (WGS) in microbial biopesticides safety assessment Role of genome sequencing (WGS) in microbial biopesticides safety assessment by Sara LAMPERTI

Next Generation Sequencing
Next Generation SequencingNext Generation Sequencing
Next Generation Sequencing
Aamir Wahab
 
MAS (MARKER ASSISTED SELECTION ) AGB PPT RAMESH KUMAR.pptx
MAS (MARKER ASSISTED SELECTION )  AGB PPT RAMESH KUMAR.pptxMAS (MARKER ASSISTED SELECTION )  AGB PPT RAMESH KUMAR.pptx
MAS (MARKER ASSISTED SELECTION ) AGB PPT RAMESH KUMAR.pptx
drrameshparmar786
 
Gene hunting strategies
Gene hunting strategiesGene hunting strategies
Gene hunting strategies
Ashfaq Ahmad
 
Application of Molecular Markers SNP and DArT in Plant Breeding: A Review Paper
Application of Molecular Markers SNP and DArT in Plant Breeding: A Review PaperApplication of Molecular Markers SNP and DArT in Plant Breeding: A Review Paper
Application of Molecular Markers SNP and DArT in Plant Breeding: A Review Paper
Journal of Agriculture and Crops
 
Molecular markers for measuring genetic diversity
Molecular markers for measuring genetic diversity Molecular markers for measuring genetic diversity
Molecular markers for measuring genetic diversity
Zohaib HUSSAIN
 
NGS Applications I (UEB-UAT Bioinformatics Course - Session 2.1.2 - VHIR, Bar...
NGS Applications I (UEB-UAT Bioinformatics Course - Session 2.1.2 - VHIR, Bar...NGS Applications I (UEB-UAT Bioinformatics Course - Session 2.1.2 - VHIR, Bar...
NGS Applications I (UEB-UAT Bioinformatics Course - Session 2.1.2 - VHIR, Bar...
VHIR Vall d’Hebron Institut de Recerca
 
Impact_of_gene_length_on_DEG
Impact_of_gene_length_on_DEGImpact_of_gene_length_on_DEG
Impact_of_gene_length_on_DEG
Long Pei
 
Gene sequencing and tb – a new age approach.pptx
Gene sequencing and tb – a new age approach.pptxGene sequencing and tb – a new age approach.pptx
Gene sequencing and tb – a new age approach.pptx
ShajahanPS
 
Sequencing and Beyond?
Sequencing and Beyond?Sequencing and Beyond?
Sequencing and Beyond?
Leighton Pritchard
 
Molecular Markers: Indispensable Tools for Genetic Diversity Analysis and Cro...
Molecular Markers: Indispensable Tools for Genetic Diversity Analysis and Cro...Molecular Markers: Indispensable Tools for Genetic Diversity Analysis and Cro...
Molecular Markers: Indispensable Tools for Genetic Diversity Analysis and Cro...
Premier Publishers
 
Sequencing-based Genotyping Assays
Sequencing-based Genotyping AssaysSequencing-based Genotyping Assays
Sequencing-based Genotyping Assays
KikoGarcia13
 
Role of molecular marker
Role of molecular markerRole of molecular marker
Role of molecular marker
Shweta Tiwari
 
Molecular approaches in improvement of fruit crops
Molecular approaches in improvement of fruit cropsMolecular approaches in improvement of fruit crops
Molecular approaches in improvement of fruit crops
ShabnamSyed3
 
THE human genome
THE human genomeTHE human genome
THE human genome
rokanuzzaman moschus
 
What are the implications of the authors findings How can this info.pdf
What are the implications of the authors findings How can this info.pdfWhat are the implications of the authors findings How can this info.pdf
What are the implications of the authors findings How can this info.pdf
amirajsharma
 
Genome sequencing. ppt.pptx
Genome sequencing. ppt.pptxGenome sequencing. ppt.pptx
Genome sequencing. ppt.pptx
GedifewGebrie
 
Molecular Markers
Molecular MarkersMolecular Markers
Genome metagenomics sequencing – next generation technology
Genome metagenomics sequencing – next generation technologyGenome metagenomics sequencing – next generation technology
Genome metagenomics sequencing – next generation technology
CD Genomics-- Biotechnology Services Company
 
Chapter 20 ppt
Chapter 20 pptChapter 20 ppt
Chapter 20 ppt
rehman2009
 
Functional genomics, and tools
Functional genomics, and toolsFunctional genomics, and tools
Functional genomics, and tools
KAUSHAL SAHU
 

Similar to Role of genome sequencing (WGS) in microbial biopesticides safety assessment Role of genome sequencing (WGS) in microbial biopesticides safety assessment by Sara LAMPERTI (20)

Next Generation Sequencing
Next Generation SequencingNext Generation Sequencing
Next Generation Sequencing
 
MAS (MARKER ASSISTED SELECTION ) AGB PPT RAMESH KUMAR.pptx
MAS (MARKER ASSISTED SELECTION )  AGB PPT RAMESH KUMAR.pptxMAS (MARKER ASSISTED SELECTION )  AGB PPT RAMESH KUMAR.pptx
MAS (MARKER ASSISTED SELECTION ) AGB PPT RAMESH KUMAR.pptx
 
Gene hunting strategies
Gene hunting strategiesGene hunting strategies
Gene hunting strategies
 
Application of Molecular Markers SNP and DArT in Plant Breeding: A Review Paper
Application of Molecular Markers SNP and DArT in Plant Breeding: A Review PaperApplication of Molecular Markers SNP and DArT in Plant Breeding: A Review Paper
Application of Molecular Markers SNP and DArT in Plant Breeding: A Review Paper
 
Molecular markers for measuring genetic diversity
Molecular markers for measuring genetic diversity Molecular markers for measuring genetic diversity
Molecular markers for measuring genetic diversity
 
NGS Applications I (UEB-UAT Bioinformatics Course - Session 2.1.2 - VHIR, Bar...
NGS Applications I (UEB-UAT Bioinformatics Course - Session 2.1.2 - VHIR, Bar...NGS Applications I (UEB-UAT Bioinformatics Course - Session 2.1.2 - VHIR, Bar...
NGS Applications I (UEB-UAT Bioinformatics Course - Session 2.1.2 - VHIR, Bar...
 
Impact_of_gene_length_on_DEG
Impact_of_gene_length_on_DEGImpact_of_gene_length_on_DEG
Impact_of_gene_length_on_DEG
 
Gene sequencing and tb – a new age approach.pptx
Gene sequencing and tb – a new age approach.pptxGene sequencing and tb – a new age approach.pptx
Gene sequencing and tb – a new age approach.pptx
 
Sequencing and Beyond?
Sequencing and Beyond?Sequencing and Beyond?
Sequencing and Beyond?
 
Molecular Markers: Indispensable Tools for Genetic Diversity Analysis and Cro...
Molecular Markers: Indispensable Tools for Genetic Diversity Analysis and Cro...Molecular Markers: Indispensable Tools for Genetic Diversity Analysis and Cro...
Molecular Markers: Indispensable Tools for Genetic Diversity Analysis and Cro...
 
Sequencing-based Genotyping Assays
Sequencing-based Genotyping AssaysSequencing-based Genotyping Assays
Sequencing-based Genotyping Assays
 
Role of molecular marker
Role of molecular markerRole of molecular marker
Role of molecular marker
 
Molecular approaches in improvement of fruit crops
Molecular approaches in improvement of fruit cropsMolecular approaches in improvement of fruit crops
Molecular approaches in improvement of fruit crops
 
THE human genome
THE human genomeTHE human genome
THE human genome
 
What are the implications of the authors findings How can this info.pdf
What are the implications of the authors findings How can this info.pdfWhat are the implications of the authors findings How can this info.pdf
What are the implications of the authors findings How can this info.pdf
 
Genome sequencing. ppt.pptx
Genome sequencing. ppt.pptxGenome sequencing. ppt.pptx
Genome sequencing. ppt.pptx
 
Molecular Markers
Molecular MarkersMolecular Markers
Molecular Markers
 
Genome metagenomics sequencing – next generation technology
Genome metagenomics sequencing – next generation technologyGenome metagenomics sequencing – next generation technology
Genome metagenomics sequencing – next generation technology
 
Chapter 20 ppt
Chapter 20 pptChapter 20 ppt
Chapter 20 ppt
 
Functional genomics, and tools
Functional genomics, and toolsFunctional genomics, and tools
Functional genomics, and tools
 

More from OECD Environment

European integration of Ukraine in the “water quality” sector
European integration of Ukraine in the “water quality” sectorEuropean integration of Ukraine in the “water quality” sector
European integration of Ukraine in the “water quality” sector
OECD Environment
 
Financing the water sector in Austria.pdf
Financing the water sector in Austria.pdfFinancing the water sector in Austria.pdf
Financing the water sector in Austria.pdf
OECD Environment
 
The Enabling Environment for Investment in Water Security.pdf
The Enabling Environment for Investment in Water Security.pdfThe Enabling Environment for Investment in Water Security.pdf
The Enabling Environment for Investment in Water Security.pdf
OECD Environment
 
AFD’s activity in EU’s Eastern Partnership Countries in a nutshell.pdf
AFD’s activity in EU’s Eastern Partnership Countries in a nutshell.pdfAFD’s activity in EU’s Eastern Partnership Countries in a nutshell.pdf
AFD’s activity in EU’s Eastern Partnership Countries in a nutshell.pdf
OECD Environment
 
Incofin’s Water Access Acceleration Fund.pdf
Incofin’s Water Access Acceleration Fund.pdfIncofin’s Water Access Acceleration Fund.pdf
Incofin’s Water Access Acceleration Fund.pdf
OECD Environment
 
Financing of River Basin Management Plans in Ukraine.pdf
Financing of River Basin Management Plans in Ukraine.pdfFinancing of River Basin Management Plans in Ukraine.pdf
Financing of River Basin Management Plans in Ukraine.pdf
OECD Environment
 
Introduction to Nature-Based Solutions.pdf
Introduction to Nature-Based Solutions.pdfIntroduction to Nature-Based Solutions.pdf
Introduction to Nature-Based Solutions.pdf
OECD Environment
 
Insights on Nature-Based Solutions from the European Commission.pdf
Insights on Nature-Based Solutions from the European Commission.pdfInsights on Nature-Based Solutions from the European Commission.pdf
Insights on Nature-Based Solutions from the European Commission.pdf
OECD Environment
 
PPTs - TAIEX TSI MNB-OECD-EC Launch Event: Technical implementation of the Su...
PPTs - TAIEX TSI MNB-OECD-EC Launch Event: Technical implementation of the Su...PPTs - TAIEX TSI MNB-OECD-EC Launch Event: Technical implementation of the Su...
PPTs - TAIEX TSI MNB-OECD-EC Launch Event: Technical implementation of the Su...
OECD Environment
 
OECD Green Talks LIVE | Diving deeper: the evolving landscape for assessing w...
OECD Green Talks LIVE | Diving deeper: the evolving landscape for assessing w...OECD Green Talks LIVE | Diving deeper: the evolving landscape for assessing w...
OECD Green Talks LIVE | Diving deeper: the evolving landscape for assessing w...
OECD Environment
 
Detlef Van Vuuren- Integrated modelling for interrelated crises.pdf
Detlef Van Vuuren- Integrated modelling for interrelated crises.pdfDetlef Van Vuuren- Integrated modelling for interrelated crises.pdf
Detlef Van Vuuren- Integrated modelling for interrelated crises.pdf
OECD Environment
 
Thomas Hertel- Integrated Policies for the Triple Planetary Crisis.pdf
Thomas Hertel- Integrated Policies for the Triple Planetary Crisis.pdfThomas Hertel- Integrated Policies for the Triple Planetary Crisis.pdf
Thomas Hertel- Integrated Policies for the Triple Planetary Crisis.pdf
OECD Environment
 
Jon Sampedro - Assessing synergies and trade offs for health and sustainable ...
Jon Sampedro - Assessing synergies and trade offs for health and sustainable ...Jon Sampedro - Assessing synergies and trade offs for health and sustainable ...
Jon Sampedro - Assessing synergies and trade offs for health and sustainable ...
OECD Environment
 
Astrid Bos - Identifying trade offs & searching for synergies.pdf
Astrid Bos - Identifying trade offs & searching for synergies.pdfAstrid Bos - Identifying trade offs & searching for synergies.pdf
Astrid Bos - Identifying trade offs & searching for synergies.pdf
OECD Environment
 
Ruth Delzeit - Modelling environmental and socio-economic impacts of cropland...
Ruth Delzeit - Modelling environmental and socio-economic impacts of cropland...Ruth Delzeit - Modelling environmental and socio-economic impacts of cropland...
Ruth Delzeit - Modelling environmental and socio-economic impacts of cropland...
OECD Environment
 
Wilfried Winiwarter - Implementing nitrogen pollution control pathways in the...
Wilfried Winiwarter - Implementing nitrogen pollution control pathways in the...Wilfried Winiwarter - Implementing nitrogen pollution control pathways in the...
Wilfried Winiwarter - Implementing nitrogen pollution control pathways in the...
OECD Environment
 
Laurent Drouet - Physical and Economic Risks of Climate Change.pdf
Laurent Drouet - Physical and Economic Risks of Climate Change.pdfLaurent Drouet - Physical and Economic Risks of Climate Change.pdf
Laurent Drouet - Physical and Economic Risks of Climate Change.pdf
OECD Environment
 
Shinichiro Fujimori - Biodiversity modeling in AIM (Asia pacific Integrated M...
Shinichiro Fujimori - Biodiversity modeling in AIM (Asia pacific Integrated M...Shinichiro Fujimori - Biodiversity modeling in AIM (Asia pacific Integrated M...
Shinichiro Fujimori - Biodiversity modeling in AIM (Asia pacific Integrated M...
OECD Environment
 
HyeJin Kim and Simon Smart - The biodiversity nexus across multiple drivers: ...
HyeJin Kim and Simon Smart - The biodiversity nexus across multiple drivers: ...HyeJin Kim and Simon Smart - The biodiversity nexus across multiple drivers: ...
HyeJin Kim and Simon Smart - The biodiversity nexus across multiple drivers: ...
OECD Environment
 
GREEN Action Task Force - Full Presentations.pdf
GREEN Action Task Force - Full Presentations.pdfGREEN Action Task Force - Full Presentations.pdf
GREEN Action Task Force - Full Presentations.pdf
OECD Environment
 

More from OECD Environment (20)

European integration of Ukraine in the “water quality” sector
European integration of Ukraine in the “water quality” sectorEuropean integration of Ukraine in the “water quality” sector
European integration of Ukraine in the “water quality” sector
 
Financing the water sector in Austria.pdf
Financing the water sector in Austria.pdfFinancing the water sector in Austria.pdf
Financing the water sector in Austria.pdf
 
The Enabling Environment for Investment in Water Security.pdf
The Enabling Environment for Investment in Water Security.pdfThe Enabling Environment for Investment in Water Security.pdf
The Enabling Environment for Investment in Water Security.pdf
 
AFD’s activity in EU’s Eastern Partnership Countries in a nutshell.pdf
AFD’s activity in EU’s Eastern Partnership Countries in a nutshell.pdfAFD’s activity in EU’s Eastern Partnership Countries in a nutshell.pdf
AFD’s activity in EU’s Eastern Partnership Countries in a nutshell.pdf
 
Incofin’s Water Access Acceleration Fund.pdf
Incofin’s Water Access Acceleration Fund.pdfIncofin’s Water Access Acceleration Fund.pdf
Incofin’s Water Access Acceleration Fund.pdf
 
Financing of River Basin Management Plans in Ukraine.pdf
Financing of River Basin Management Plans in Ukraine.pdfFinancing of River Basin Management Plans in Ukraine.pdf
Financing of River Basin Management Plans in Ukraine.pdf
 
Introduction to Nature-Based Solutions.pdf
Introduction to Nature-Based Solutions.pdfIntroduction to Nature-Based Solutions.pdf
Introduction to Nature-Based Solutions.pdf
 
Insights on Nature-Based Solutions from the European Commission.pdf
Insights on Nature-Based Solutions from the European Commission.pdfInsights on Nature-Based Solutions from the European Commission.pdf
Insights on Nature-Based Solutions from the European Commission.pdf
 
PPTs - TAIEX TSI MNB-OECD-EC Launch Event: Technical implementation of the Su...
PPTs - TAIEX TSI MNB-OECD-EC Launch Event: Technical implementation of the Su...PPTs - TAIEX TSI MNB-OECD-EC Launch Event: Technical implementation of the Su...
PPTs - TAIEX TSI MNB-OECD-EC Launch Event: Technical implementation of the Su...
 
OECD Green Talks LIVE | Diving deeper: the evolving landscape for assessing w...
OECD Green Talks LIVE | Diving deeper: the evolving landscape for assessing w...OECD Green Talks LIVE | Diving deeper: the evolving landscape for assessing w...
OECD Green Talks LIVE | Diving deeper: the evolving landscape for assessing w...
 
Detlef Van Vuuren- Integrated modelling for interrelated crises.pdf
Detlef Van Vuuren- Integrated modelling for interrelated crises.pdfDetlef Van Vuuren- Integrated modelling for interrelated crises.pdf
Detlef Van Vuuren- Integrated modelling for interrelated crises.pdf
 
Thomas Hertel- Integrated Policies for the Triple Planetary Crisis.pdf
Thomas Hertel- Integrated Policies for the Triple Planetary Crisis.pdfThomas Hertel- Integrated Policies for the Triple Planetary Crisis.pdf
Thomas Hertel- Integrated Policies for the Triple Planetary Crisis.pdf
 
Jon Sampedro - Assessing synergies and trade offs for health and sustainable ...
Jon Sampedro - Assessing synergies and trade offs for health and sustainable ...Jon Sampedro - Assessing synergies and trade offs for health and sustainable ...
Jon Sampedro - Assessing synergies and trade offs for health and sustainable ...
 
Astrid Bos - Identifying trade offs & searching for synergies.pdf
Astrid Bos - Identifying trade offs & searching for synergies.pdfAstrid Bos - Identifying trade offs & searching for synergies.pdf
Astrid Bos - Identifying trade offs & searching for synergies.pdf
 
Ruth Delzeit - Modelling environmental and socio-economic impacts of cropland...
Ruth Delzeit - Modelling environmental and socio-economic impacts of cropland...Ruth Delzeit - Modelling environmental and socio-economic impacts of cropland...
Ruth Delzeit - Modelling environmental and socio-economic impacts of cropland...
 
Wilfried Winiwarter - Implementing nitrogen pollution control pathways in the...
Wilfried Winiwarter - Implementing nitrogen pollution control pathways in the...Wilfried Winiwarter - Implementing nitrogen pollution control pathways in the...
Wilfried Winiwarter - Implementing nitrogen pollution control pathways in the...
 
Laurent Drouet - Physical and Economic Risks of Climate Change.pdf
Laurent Drouet - Physical and Economic Risks of Climate Change.pdfLaurent Drouet - Physical and Economic Risks of Climate Change.pdf
Laurent Drouet - Physical and Economic Risks of Climate Change.pdf
 
Shinichiro Fujimori - Biodiversity modeling in AIM (Asia pacific Integrated M...
Shinichiro Fujimori - Biodiversity modeling in AIM (Asia pacific Integrated M...Shinichiro Fujimori - Biodiversity modeling in AIM (Asia pacific Integrated M...
Shinichiro Fujimori - Biodiversity modeling in AIM (Asia pacific Integrated M...
 
HyeJin Kim and Simon Smart - The biodiversity nexus across multiple drivers: ...
HyeJin Kim and Simon Smart - The biodiversity nexus across multiple drivers: ...HyeJin Kim and Simon Smart - The biodiversity nexus across multiple drivers: ...
HyeJin Kim and Simon Smart - The biodiversity nexus across multiple drivers: ...
 
GREEN Action Task Force - Full Presentations.pdf
GREEN Action Task Force - Full Presentations.pdfGREEN Action Task Force - Full Presentations.pdf
GREEN Action Task Force - Full Presentations.pdf
 

Recently uploaded

原版制作(Newcastle毕业证书)纽卡斯尔大学毕业证在读证明一模一样
原版制作(Newcastle毕业证书)纽卡斯尔大学毕业证在读证明一模一样原版制作(Newcastle毕业证书)纽卡斯尔大学毕业证在读证明一模一样
原版制作(Newcastle毕业证书)纽卡斯尔大学毕业证在读证明一模一样
p2npnqp
 
Optimizing Post Remediation Groundwater Performance with Enhanced Microbiolog...
Optimizing Post Remediation Groundwater Performance with Enhanced Microbiolog...Optimizing Post Remediation Groundwater Performance with Enhanced Microbiolog...
Optimizing Post Remediation Groundwater Performance with Enhanced Microbiolog...
Joshua Orris
 
Kinetic studies on malachite green dye adsorption from aqueous solutions by A...
Kinetic studies on malachite green dye adsorption from aqueous solutions by A...Kinetic studies on malachite green dye adsorption from aqueous solutions by A...
Kinetic studies on malachite green dye adsorption from aqueous solutions by A...
Open Access Research Paper
 
Biomimicry in agriculture: Nature-Inspired Solutions for a Greener Future
Biomimicry in agriculture: Nature-Inspired Solutions for a Greener FutureBiomimicry in agriculture: Nature-Inspired Solutions for a Greener Future
Biomimicry in agriculture: Nature-Inspired Solutions for a Greener Future
Dr. P.B.Dharmasena
 
world-environment-day-2024-240601103559-14f4c0b4.pptx
world-environment-day-2024-240601103559-14f4c0b4.pptxworld-environment-day-2024-240601103559-14f4c0b4.pptx
world-environment-day-2024-240601103559-14f4c0b4.pptx
mfasna35
 
在线办理(lboro毕业证书)拉夫堡大学毕业证学历证书一模一样
在线办理(lboro毕业证书)拉夫堡大学毕业证学历证书一模一样在线办理(lboro毕业证书)拉夫堡大学毕业证学历证书一模一样
在线办理(lboro毕业证书)拉夫堡大学毕业证学历证书一模一样
pjq9n1lk
 
BASIC CONCEPT OF ENVIRONMENT AND DIFFERENT CONSTITUTENET OF ENVIRONMENT
BASIC CONCEPT OF ENVIRONMENT AND DIFFERENT CONSTITUTENET OF ENVIRONMENTBASIC CONCEPT OF ENVIRONMENT AND DIFFERENT CONSTITUTENET OF ENVIRONMENT
BASIC CONCEPT OF ENVIRONMENT AND DIFFERENT CONSTITUTENET OF ENVIRONMENT
AmitKumar619042
 
Evolving Lifecycles with High Resolution Site Characterization (HRSC) and 3-D...
Evolving Lifecycles with High Resolution Site Characterization (HRSC) and 3-D...Evolving Lifecycles with High Resolution Site Characterization (HRSC) and 3-D...
Evolving Lifecycles with High Resolution Site Characterization (HRSC) and 3-D...
Joshua Orris
 
REPORT-PRESENTATION BY CHIEF SECRETARY, ANDAMAN NICOBAR ADMINISTRATION IN OA ...
REPORT-PRESENTATION BY CHIEF SECRETARY, ANDAMAN NICOBAR ADMINISTRATION IN OA ...REPORT-PRESENTATION BY CHIEF SECRETARY, ANDAMAN NICOBAR ADMINISTRATION IN OA ...
REPORT-PRESENTATION BY CHIEF SECRETARY, ANDAMAN NICOBAR ADMINISTRATION IN OA ...
pareeksulkash
 
学校原版(unuk学位证书)英国牛津布鲁克斯大学毕业证硕士文凭原版一模一样
学校原版(unuk学位证书)英国牛津布鲁克斯大学毕业证硕士文凭原版一模一样学校原版(unuk学位证书)英国牛津布鲁克斯大学毕业证硕士文凭原版一模一样
学校原版(unuk学位证书)英国牛津布鲁克斯大学毕业证硕士文凭原版一模一样
ehfyqtu
 
Lessons from operationalizing integrated landscape approaches
Lessons from operationalizing integrated landscape approachesLessons from operationalizing integrated landscape approaches
Lessons from operationalizing integrated landscape approaches
CIFOR-ICRAF
 
Improving the viability of probiotics by encapsulation methods for developmen...
Improving the viability of probiotics by encapsulation methods for developmen...Improving the viability of probiotics by encapsulation methods for developmen...
Improving the viability of probiotics by encapsulation methods for developmen...
Open Access Research Paper
 
快速办理(Calabria毕业证书)卡拉布里亚大学毕业证在读证明一模一样
快速办理(Calabria毕业证书)卡拉布里亚大学毕业证在读证明一模一样快速办理(Calabria毕业证书)卡拉布里亚大学毕业证在读证明一模一样
快速办理(Calabria毕业证书)卡拉布里亚大学毕业证在读证明一模一样
astuz
 
一比一原版西澳大学毕业证学历证书如何办理
一比一原版西澳大学毕业证学历证书如何办理一比一原版西澳大学毕业证学历证书如何办理
一比一原版西澳大学毕业证学历证书如何办理
yxfus
 
原版制作(Manitoba毕业证书)曼尼托巴大学毕业证学位证一模一样
原版制作(Manitoba毕业证书)曼尼托巴大学毕业证学位证一模一样原版制作(Manitoba毕业证书)曼尼托巴大学毕业证学位证一模一样
原版制作(Manitoba毕业证书)曼尼托巴大学毕业证学位证一模一样
mvrpcz6
 
Environment Conservation Rules 2023 (ECR)-2023.pptx
Environment Conservation Rules 2023 (ECR)-2023.pptxEnvironment Conservation Rules 2023 (ECR)-2023.pptx
Environment Conservation Rules 2023 (ECR)-2023.pptx
neilsencassidy
 
PACKAGING OF FROZEN FOODS ( food technology)
PACKAGING OF FROZEN FOODS  ( food technology)PACKAGING OF FROZEN FOODS  ( food technology)
PACKAGING OF FROZEN FOODS ( food technology)
Addu25809
 
按照学校原版(UAL文凭证书)伦敦艺术大学毕业证快速办理
按照学校原版(UAL文凭证书)伦敦艺术大学毕业证快速办理按照学校原版(UAL文凭证书)伦敦艺术大学毕业证快速办理
按照学校原版(UAL文凭证书)伦敦艺术大学毕业证快速办理
xeexm
 

Recently uploaded (18)

原版制作(Newcastle毕业证书)纽卡斯尔大学毕业证在读证明一模一样
原版制作(Newcastle毕业证书)纽卡斯尔大学毕业证在读证明一模一样原版制作(Newcastle毕业证书)纽卡斯尔大学毕业证在读证明一模一样
原版制作(Newcastle毕业证书)纽卡斯尔大学毕业证在读证明一模一样
 
Optimizing Post Remediation Groundwater Performance with Enhanced Microbiolog...
Optimizing Post Remediation Groundwater Performance with Enhanced Microbiolog...Optimizing Post Remediation Groundwater Performance with Enhanced Microbiolog...
Optimizing Post Remediation Groundwater Performance with Enhanced Microbiolog...
 
Kinetic studies on malachite green dye adsorption from aqueous solutions by A...
Kinetic studies on malachite green dye adsorption from aqueous solutions by A...Kinetic studies on malachite green dye adsorption from aqueous solutions by A...
Kinetic studies on malachite green dye adsorption from aqueous solutions by A...
 
Biomimicry in agriculture: Nature-Inspired Solutions for a Greener Future
Biomimicry in agriculture: Nature-Inspired Solutions for a Greener FutureBiomimicry in agriculture: Nature-Inspired Solutions for a Greener Future
Biomimicry in agriculture: Nature-Inspired Solutions for a Greener Future
 
world-environment-day-2024-240601103559-14f4c0b4.pptx
world-environment-day-2024-240601103559-14f4c0b4.pptxworld-environment-day-2024-240601103559-14f4c0b4.pptx
world-environment-day-2024-240601103559-14f4c0b4.pptx
 
在线办理(lboro毕业证书)拉夫堡大学毕业证学历证书一模一样
在线办理(lboro毕业证书)拉夫堡大学毕业证学历证书一模一样在线办理(lboro毕业证书)拉夫堡大学毕业证学历证书一模一样
在线办理(lboro毕业证书)拉夫堡大学毕业证学历证书一模一样
 
BASIC CONCEPT OF ENVIRONMENT AND DIFFERENT CONSTITUTENET OF ENVIRONMENT
BASIC CONCEPT OF ENVIRONMENT AND DIFFERENT CONSTITUTENET OF ENVIRONMENTBASIC CONCEPT OF ENVIRONMENT AND DIFFERENT CONSTITUTENET OF ENVIRONMENT
BASIC CONCEPT OF ENVIRONMENT AND DIFFERENT CONSTITUTENET OF ENVIRONMENT
 
Evolving Lifecycles with High Resolution Site Characterization (HRSC) and 3-D...
Evolving Lifecycles with High Resolution Site Characterization (HRSC) and 3-D...Evolving Lifecycles with High Resolution Site Characterization (HRSC) and 3-D...
Evolving Lifecycles with High Resolution Site Characterization (HRSC) and 3-D...
 
REPORT-PRESENTATION BY CHIEF SECRETARY, ANDAMAN NICOBAR ADMINISTRATION IN OA ...
REPORT-PRESENTATION BY CHIEF SECRETARY, ANDAMAN NICOBAR ADMINISTRATION IN OA ...REPORT-PRESENTATION BY CHIEF SECRETARY, ANDAMAN NICOBAR ADMINISTRATION IN OA ...
REPORT-PRESENTATION BY CHIEF SECRETARY, ANDAMAN NICOBAR ADMINISTRATION IN OA ...
 
学校原版(unuk学位证书)英国牛津布鲁克斯大学毕业证硕士文凭原版一模一样
学校原版(unuk学位证书)英国牛津布鲁克斯大学毕业证硕士文凭原版一模一样学校原版(unuk学位证书)英国牛津布鲁克斯大学毕业证硕士文凭原版一模一样
学校原版(unuk学位证书)英国牛津布鲁克斯大学毕业证硕士文凭原版一模一样
 
Lessons from operationalizing integrated landscape approaches
Lessons from operationalizing integrated landscape approachesLessons from operationalizing integrated landscape approaches
Lessons from operationalizing integrated landscape approaches
 
Improving the viability of probiotics by encapsulation methods for developmen...
Improving the viability of probiotics by encapsulation methods for developmen...Improving the viability of probiotics by encapsulation methods for developmen...
Improving the viability of probiotics by encapsulation methods for developmen...
 
快速办理(Calabria毕业证书)卡拉布里亚大学毕业证在读证明一模一样
快速办理(Calabria毕业证书)卡拉布里亚大学毕业证在读证明一模一样快速办理(Calabria毕业证书)卡拉布里亚大学毕业证在读证明一模一样
快速办理(Calabria毕业证书)卡拉布里亚大学毕业证在读证明一模一样
 
一比一原版西澳大学毕业证学历证书如何办理
一比一原版西澳大学毕业证学历证书如何办理一比一原版西澳大学毕业证学历证书如何办理
一比一原版西澳大学毕业证学历证书如何办理
 
原版制作(Manitoba毕业证书)曼尼托巴大学毕业证学位证一模一样
原版制作(Manitoba毕业证书)曼尼托巴大学毕业证学位证一模一样原版制作(Manitoba毕业证书)曼尼托巴大学毕业证学位证一模一样
原版制作(Manitoba毕业证书)曼尼托巴大学毕业证学位证一模一样
 
Environment Conservation Rules 2023 (ECR)-2023.pptx
Environment Conservation Rules 2023 (ECR)-2023.pptxEnvironment Conservation Rules 2023 (ECR)-2023.pptx
Environment Conservation Rules 2023 (ECR)-2023.pptx
 
PACKAGING OF FROZEN FOODS ( food technology)
PACKAGING OF FROZEN FOODS  ( food technology)PACKAGING OF FROZEN FOODS  ( food technology)
PACKAGING OF FROZEN FOODS ( food technology)
 
按照学校原版(UAL文凭证书)伦敦艺术大学毕业证快速办理
按照学校原版(UAL文凭证书)伦敦艺术大学毕业证快速办理按照学校原版(UAL文凭证书)伦敦艺术大学毕业证快速办理
按照学校原版(UAL文凭证书)伦敦艺术大学毕业证快速办理
 

Role of genome sequencing (WGS) in microbial biopesticides safety assessment Role of genome sequencing (WGS) in microbial biopesticides safety assessment by Sara LAMPERTI

  • 1. Role of WGS in microbial biopesticides safety assessment Sara Lamperti Global Regulatory Toxicologist Corteva Agriscience Crop Life International February 26, 2024
  • 2. Whole genome sequencing (WGS) What is it? Whole genome sequencing (WGS) is a process to determine the complete DNA sequence of an organism's genome, using high-throughput sequencing technologies. It provides a comprehensive view of an organism's genetic makeup. Methods: Next-generation sequencing (NGS): short reads (most common) Single-Molecule Real-Time (SMRT) sequencing: Long reads (better choice) Nanopore sequencing: Long reads and portable 2 Limits: availability of correctly annotated sequence information Advantages: • to help the evaluation/registrability of the microorganisms • to reduce the animal testing
  • 3. Whole genome sequence (WGS) Scope : 3 ➢ Identify microorganism at strain level ➢ To confirm the absence of known genes involved in pathogenicity (cluster toxins) ➢ Phylogenetic tree to show the strain is phylogenetically away from pathogenic strains ➢ Identification antimicrobial resistance (AMR) genes ➢ Explore genome to predict secondary metabolites or exclude the metabolites of potential concern
  • 4. Example of evaluation of the potential production of toxin X by a fungus strain 4 Query Target Strain Similarity (%) Toxigenic strain* Similarity (%) Gene 1 99.59 99.34 Gene 2 34.58 99.80 Gene 3 35.47 99.48 Gene 4 42.17 98.13 Gene 5 29.84 98.56 Gene 6 38.85 97.40 Gene 7 39.17 97.57 Gene 8 53.15 100 Gene 9 37.60 99.62 Gene 10 41.28 99.68 Gene 11 42.93 99.94 Gene 12 39.92 99.95 Gene 13 39.52 78.91 * is a known toxin X producing strain used as a positive control Conclusion: only 1 of 13 toxin X production pathway genes was found in target strain’s genome with high similarity. In contrast, all 13 genes were found in the toxigenic strain with high similarity. The target strain is very unlikely can produce toxin X Blast toxin X biosynthetic gene clusters against target strain and known toxigenic strain’s genomes
  • 5. 5 * Special Thank You To Corteva Colleague Xiao (Eric) Huang