Quantitative Methods and Numerical Problem Solving in Microbiology for Biologists

Quantitative Methods and Numerical Problem Solving in Microbiology for Biologists

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  By Mrs. Sanchita Choubey AssistantProfessor of Microbiology Dr. D. Y. Patil Arts Commerce and Science College , Pimpri, Pune Quantitative Approach for Biologists
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  Quantitative Approach forBiologists 1. Numerical Microbiology: Problem solving: Unit conversion, preparation of standard solutions (Wt/V, V/V, %, Normal solution, Molar solutions etc.), Numerical Problems on size, volume, number (CFU and PFU), dilutions, Neubauer chamber, direct Microscopic count, Numerical Problems on Bacterial Growth. Numerical problems on diversity indices.
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  Solutions & UnitConversions Foundation metrics, stoichiometric ratios, concentration expressions, and accurate stock preparation in laboratory microbiology.
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  1. Unit Conversionsand Solution Preparation 1.1. Common Metric Unit Conversions in Microbiology
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  1.2. Solution ConcentrationFormulations Percentage Solutions
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  Molar and NormalSolutions
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  Neubauer Chamber &Direct Counts Direct Microscopic Enumeration The Improved Neubauer hemocytometer provides a fixed volume chamber (depth = 0.1 mm) for direct cell counting. Grid volume for one large square (1 mm² area) is 0.1 mm³ ( ), yielding the multiplier .
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  Simpson's Index (λor D):