SlideShare a Scribd company logo
1 of 11
Download to read offline
Mariana Arango Perez
Tercer semestre
UPB
Is a circular non-coding RNA that acts as a tumor suppressor in pancreatic
ductal adenocarcinoma (PDAC) by regulating the miR-147b/SOCS1 axis,
inhibiting cell proliferation, invasion, and epithelial-mesenchymal transition
(EMT).
INTRODUCTION
This RNA molecule plays a crucial role in the pathogenesis of PDAC
and may serve as a potential therapeutic target for this aggressive
cancer.
CIRCTMEM-59
This is the most lethal tumor in the world, with a five-year
survival rate less than 8.5%
INTRODUCTION
It is the most common type of pancreatic cancer and is known
for its aggressive nature and poor prognosis, often diagnosed at
advanced stages
PDAC
Investigate how circular RNA
circTMEM59 inhibits progression of
pancreatic ductal adenocarcinoma by
targeting miR-147b/SOCS1
GENERAL
OBJETIVE
METHODS
Used to introduce circTMEM59 mimics, miR-
147b mimics, miR-147b inhibitors, si-SOCS1,
and corresponding negative controls into
PANC-1
BXPC-3 cells
QRT-PCR was used to analyze
gene expression in
GAPDH and
U6 serving as internal
controls
(QRT-PCR)
CELL TRANSFECTION
Used to:
measure the expression of different
PDAC cells when being exposed to
diferent RNAs as circTMEM-59
METHODS
In this assay, the luciferase gene is
fused to a gene of interest, and the
resulting construct is transfected
into cells.
WESTERN BLOTTING
LUCIFERASE REPORTER ASSAY
RESULTS
FIG 2
D
RESULTS
FIG 5
FIG 4 FIG 6
DISCUSSION
By understanding the molecular mechanisms underlying diseases,
Doctors can now prescribe medications and therapies with greater
accuracy, minimizing adverse effects and optimizing therapeutic
outcomes
Molecular biology has revolutionized cancer treatment by
uncovering the molecular mechanisms driving tumor growth and
progression.
CONCLUSIONS
1
1
2
ADENOCARCINOMA DUCTAL DE
PANCREAS
es
investigacion
Inhibe Activa
Este participa en
Considerado uno de los tumores mas
letales
CircTMEM-59
MiR-147b
Este participa en
SOCS-1
proliferacion invasion transicion supresion regulación de genes
PDAC PDAC
Se detiene la progresión del
cancer
En En
Al inhibirse Al activarse

More Related Content

Similar to Presentacion Mariana Arango- biología molecular

Serum tumor markers
Serum tumor markersSerum tumor markers
Serum tumor markersLAB IDEA
 
Rnases in anti-Viral and anti-Cancer therapy
Rnases in anti-Viral and anti-Cancer therapyRnases in anti-Viral and anti-Cancer therapy
Rnases in anti-Viral and anti-Cancer therapyAsim Abbasi
 
EARLY Renal Cell Cancer Management
EARLY Renal Cell Cancer ManagementEARLY Renal Cell Cancer Management
EARLY Renal Cell Cancer ManagementSeraj Aldeen
 
High Sensitivity Detection of Tumor Gene Mutations-v3
High Sensitivity Detection of Tumor Gene Mutations-v3High Sensitivity Detection of Tumor Gene Mutations-v3
High Sensitivity Detection of Tumor Gene Mutations-v3Michael Powell
 
Side Effects Management for the Ovarian Cancer Community
Side Effects Management for the Ovarian Cancer CommunitySide Effects Management for the Ovarian Cancer Community
Side Effects Management for the Ovarian Cancer Communitybkling
 
Canine oncoprotein targets for Melanoma, Breast Cancer, Osteosarcoma
Canine oncoprotein targets for Melanoma, Breast Cancer, OsteosarcomaCanine oncoprotein targets for Melanoma, Breast Cancer, Osteosarcoma
Canine oncoprotein targets for Melanoma, Breast Cancer, OsteosarcomaSnehal Salunkhe
 
Genetic instability(One of the Hallmarks) and Treatment options
Genetic instability(One of the Hallmarks) and Treatment optionsGenetic instability(One of the Hallmarks) and Treatment options
Genetic instability(One of the Hallmarks) and Treatment optionsAmir Hossain
 
Colorectal carcinoma gist carcinoid tumour
Colorectal carcinoma  gist carcinoid tumourColorectal carcinoma  gist carcinoid tumour
Colorectal carcinoma gist carcinoid tumourSrabani chakrabarti
 
Crimson Publishers-SiRNA Delivery for Cancer Therapy: Challenges and Future P...
Crimson Publishers-SiRNA Delivery for Cancer Therapy: Challenges and Future P...Crimson Publishers-SiRNA Delivery for Cancer Therapy: Challenges and Future P...
Crimson Publishers-SiRNA Delivery for Cancer Therapy: Challenges and Future P...CrimsonPublishersBioavailability
 
Arpin downregulation is associated with poor prognosis in pancreatic ductal a...
Arpin downregulation is associated with poor prognosis in pancreatic ductal a...Arpin downregulation is associated with poor prognosis in pancreatic ductal a...
Arpin downregulation is associated with poor prognosis in pancreatic ductal a...MarianaBarona
 
LUNG CANCER TREATMENT BY NIVOLUMAB
LUNG CANCER TREATMENT BY NIVOLUMABLUNG CANCER TREATMENT BY NIVOLUMAB
LUNG CANCER TREATMENT BY NIVOLUMABHeena Parveen
 
Pancreatic cancer (6 October 2014)
Pancreatic cancer (6 October 2014)Pancreatic cancer (6 October 2014)
Pancreatic cancer (6 October 2014)Zeena Nackerdien
 
Biomarker in cancer
Biomarker in cancerBiomarker in cancer
Biomarker in cancermanojjeya
 
IATTGI Educational Lecture on pancreatic cancer by Eric Raymond in Buenos Air...
IATTGI Educational Lecture on pancreatic cancer by Eric Raymond in Buenos Air...IATTGI Educational Lecture on pancreatic cancer by Eric Raymond in Buenos Air...
IATTGI Educational Lecture on pancreatic cancer by Eric Raymond in Buenos Air...Prof. Eric Raymond Oncologie Medicale
 

Similar to Presentacion Mariana Arango- biología molecular (20)

SEMINARIO BIOLOGÍA M.pdf
SEMINARIO BIOLOGÍA M.pdfSEMINARIO BIOLOGÍA M.pdf
SEMINARIO BIOLOGÍA M.pdf
 
Serum tumor markers
Serum tumor markersSerum tumor markers
Serum tumor markers
 
Rnases in anti-Viral and anti-Cancer therapy
Rnases in anti-Viral and anti-Cancer therapyRnases in anti-Viral and anti-Cancer therapy
Rnases in anti-Viral and anti-Cancer therapy
 
Gene therapy
Gene therapyGene therapy
Gene therapy
 
EARLY Renal Cell Cancer Management
EARLY Renal Cell Cancer ManagementEARLY Renal Cell Cancer Management
EARLY Renal Cell Cancer Management
 
High Sensitivity Detection of Tumor Gene Mutations-v3
High Sensitivity Detection of Tumor Gene Mutations-v3High Sensitivity Detection of Tumor Gene Mutations-v3
High Sensitivity Detection of Tumor Gene Mutations-v3
 
Dassie et al. Mol Ther 2014
Dassie et al. Mol Ther 2014Dassie et al. Mol Ther 2014
Dassie et al. Mol Ther 2014
 
Side Effects Management for the Ovarian Cancer Community
Side Effects Management for the Ovarian Cancer CommunitySide Effects Management for the Ovarian Cancer Community
Side Effects Management for the Ovarian Cancer Community
 
Canine oncoprotein targets for Melanoma, Breast Cancer, Osteosarcoma
Canine oncoprotein targets for Melanoma, Breast Cancer, OsteosarcomaCanine oncoprotein targets for Melanoma, Breast Cancer, Osteosarcoma
Canine oncoprotein targets for Melanoma, Breast Cancer, Osteosarcoma
 
New Concepts in Prostate Cancer Care: What Oncologists Need to Know to Optimi...
New Concepts in Prostate Cancer Care: What Oncologists Need to Know to Optimi...New Concepts in Prostate Cancer Care: What Oncologists Need to Know to Optimi...
New Concepts in Prostate Cancer Care: What Oncologists Need to Know to Optimi...
 
Genetic instability(One of the Hallmarks) and Treatment options
Genetic instability(One of the Hallmarks) and Treatment optionsGenetic instability(One of the Hallmarks) and Treatment options
Genetic instability(One of the Hallmarks) and Treatment options
 
Cancer Genetics (Genes of cancer)
Cancer Genetics (Genes of cancer)Cancer Genetics (Genes of cancer)
Cancer Genetics (Genes of cancer)
 
Rapamycin
RapamycinRapamycin
Rapamycin
 
Colorectal carcinoma gist carcinoid tumour
Colorectal carcinoma  gist carcinoid tumourColorectal carcinoma  gist carcinoid tumour
Colorectal carcinoma gist carcinoid tumour
 
Crimson Publishers-SiRNA Delivery for Cancer Therapy: Challenges and Future P...
Crimson Publishers-SiRNA Delivery for Cancer Therapy: Challenges and Future P...Crimson Publishers-SiRNA Delivery for Cancer Therapy: Challenges and Future P...
Crimson Publishers-SiRNA Delivery for Cancer Therapy: Challenges and Future P...
 
Arpin downregulation is associated with poor prognosis in pancreatic ductal a...
Arpin downregulation is associated with poor prognosis in pancreatic ductal a...Arpin downregulation is associated with poor prognosis in pancreatic ductal a...
Arpin downregulation is associated with poor prognosis in pancreatic ductal a...
 
LUNG CANCER TREATMENT BY NIVOLUMAB
LUNG CANCER TREATMENT BY NIVOLUMABLUNG CANCER TREATMENT BY NIVOLUMAB
LUNG CANCER TREATMENT BY NIVOLUMAB
 
Pancreatic cancer (6 October 2014)
Pancreatic cancer (6 October 2014)Pancreatic cancer (6 October 2014)
Pancreatic cancer (6 October 2014)
 
Biomarker in cancer
Biomarker in cancerBiomarker in cancer
Biomarker in cancer
 
IATTGI Educational Lecture on pancreatic cancer by Eric Raymond in Buenos Air...
IATTGI Educational Lecture on pancreatic cancer by Eric Raymond in Buenos Air...IATTGI Educational Lecture on pancreatic cancer by Eric Raymond in Buenos Air...
IATTGI Educational Lecture on pancreatic cancer by Eric Raymond in Buenos Air...
 

Recently uploaded

Four Spheres of the Earth Presentation.ppt
Four Spheres of the Earth Presentation.pptFour Spheres of the Earth Presentation.ppt
Four Spheres of the Earth Presentation.pptJoemSTuliba
 
PPT Measures of protein quality.pptx in monogastric
PPT Measures of protein quality.pptx in monogastricPPT Measures of protein quality.pptx in monogastric
PPT Measures of protein quality.pptx in monogastricSonaliNamdeo1
 
The dark energy paradox leads to a new structure of spacetime.pptx
The dark energy paradox leads to a new structure of spacetime.pptxThe dark energy paradox leads to a new structure of spacetime.pptx
The dark energy paradox leads to a new structure of spacetime.pptxEran Akiva Sinbar
 
trihybrid cross , test cross chi squares
trihybrid cross , test cross chi squarestrihybrid cross , test cross chi squares
trihybrid cross , test cross chi squaresusmanzain586
 
Call Girls in Majnu Ka Tilla Delhi 🔝9711014705🔝 Genuine
Call Girls in Majnu Ka Tilla Delhi 🔝9711014705🔝 GenuineCall Girls in Majnu Ka Tilla Delhi 🔝9711014705🔝 Genuine
Call Girls in Majnu Ka Tilla Delhi 🔝9711014705🔝 Genuinethapagita
 
User Guide: Pulsar™ Weather Station (Columbia Weather Systems)
User Guide: Pulsar™ Weather Station (Columbia Weather Systems)User Guide: Pulsar™ Weather Station (Columbia Weather Systems)
User Guide: Pulsar™ Weather Station (Columbia Weather Systems)Columbia Weather Systems
 
Observational constraints on mergers creating magnetism in massive stars
Observational constraints on mergers creating magnetism in massive starsObservational constraints on mergers creating magnetism in massive stars
Observational constraints on mergers creating magnetism in massive starsSérgio Sacani
 
Introduction of Human Body & Structure of cell.pptx
Introduction of Human Body & Structure of cell.pptxIntroduction of Human Body & Structure of cell.pptx
Introduction of Human Body & Structure of cell.pptxMedical College
 
Q4-Mod-1c-Quiz-Projectile-333344444.pptx
Q4-Mod-1c-Quiz-Projectile-333344444.pptxQ4-Mod-1c-Quiz-Projectile-333344444.pptx
Q4-Mod-1c-Quiz-Projectile-333344444.pptxtuking87
 
Dubai Calls Girl Lisa O525547819 Lexi Call Girls In Dubai
Dubai Calls Girl Lisa O525547819 Lexi Call Girls In DubaiDubai Calls Girl Lisa O525547819 Lexi Call Girls In Dubai
Dubai Calls Girl Lisa O525547819 Lexi Call Girls In Dubaikojalkojal131
 
The Sensory Organs, Anatomy and Function
The Sensory Organs, Anatomy and FunctionThe Sensory Organs, Anatomy and Function
The Sensory Organs, Anatomy and FunctionJadeNovelo1
 
Pests of castor_Binomics_Identification_Dr.UPR.pdf
Pests of castor_Binomics_Identification_Dr.UPR.pdfPests of castor_Binomics_Identification_Dr.UPR.pdf
Pests of castor_Binomics_Identification_Dr.UPR.pdfPirithiRaju
 
Pests of soyabean_Binomics_IdentificationDr.UPR.pdf
Pests of soyabean_Binomics_IdentificationDr.UPR.pdfPests of soyabean_Binomics_IdentificationDr.UPR.pdf
Pests of soyabean_Binomics_IdentificationDr.UPR.pdfPirithiRaju
 
Pests of jatropha_Bionomics_identification_Dr.UPR.pdf
Pests of jatropha_Bionomics_identification_Dr.UPR.pdfPests of jatropha_Bionomics_identification_Dr.UPR.pdf
Pests of jatropha_Bionomics_identification_Dr.UPR.pdfPirithiRaju
 
Loudspeaker- direct radiating type and horn type.pptx
Loudspeaker- direct radiating type and horn type.pptxLoudspeaker- direct radiating type and horn type.pptx
Loudspeaker- direct radiating type and horn type.pptxpriyankatabhane
 
Base editing, prime editing, Cas13 & RNA editing and organelle base editing
Base editing, prime editing, Cas13 & RNA editing and organelle base editingBase editing, prime editing, Cas13 & RNA editing and organelle base editing
Base editing, prime editing, Cas13 & RNA editing and organelle base editingNetHelix
 
REVISTA DE BIOLOGIA E CIÊNCIAS DA TERRA ISSN 1519-5228 - Artigo_Bioterra_V24_...
REVISTA DE BIOLOGIA E CIÊNCIAS DA TERRA ISSN 1519-5228 - Artigo_Bioterra_V24_...REVISTA DE BIOLOGIA E CIÊNCIAS DA TERRA ISSN 1519-5228 - Artigo_Bioterra_V24_...
REVISTA DE BIOLOGIA E CIÊNCIAS DA TERRA ISSN 1519-5228 - Artigo_Bioterra_V24_...Universidade Federal de Sergipe - UFS
 
Gas-ExchangeS-in-Plants-and-Animals.pptx
Gas-ExchangeS-in-Plants-and-Animals.pptxGas-ExchangeS-in-Plants-and-Animals.pptx
Gas-ExchangeS-in-Plants-and-Animals.pptxGiovaniTrinidad
 
CHROMATOGRAPHY PALLAVI RAWAT.pptx
CHROMATOGRAPHY  PALLAVI RAWAT.pptxCHROMATOGRAPHY  PALLAVI RAWAT.pptx
CHROMATOGRAPHY PALLAVI RAWAT.pptxpallavirawat456
 

Recently uploaded (20)

Four Spheres of the Earth Presentation.ppt
Four Spheres of the Earth Presentation.pptFour Spheres of the Earth Presentation.ppt
Four Spheres of the Earth Presentation.ppt
 
PPT Measures of protein quality.pptx in monogastric
PPT Measures of protein quality.pptx in monogastricPPT Measures of protein quality.pptx in monogastric
PPT Measures of protein quality.pptx in monogastric
 
The dark energy paradox leads to a new structure of spacetime.pptx
The dark energy paradox leads to a new structure of spacetime.pptxThe dark energy paradox leads to a new structure of spacetime.pptx
The dark energy paradox leads to a new structure of spacetime.pptx
 
trihybrid cross , test cross chi squares
trihybrid cross , test cross chi squarestrihybrid cross , test cross chi squares
trihybrid cross , test cross chi squares
 
PLASMODIUM. PPTX
PLASMODIUM. PPTXPLASMODIUM. PPTX
PLASMODIUM. PPTX
 
Call Girls in Majnu Ka Tilla Delhi 🔝9711014705🔝 Genuine
Call Girls in Majnu Ka Tilla Delhi 🔝9711014705🔝 GenuineCall Girls in Majnu Ka Tilla Delhi 🔝9711014705🔝 Genuine
Call Girls in Majnu Ka Tilla Delhi 🔝9711014705🔝 Genuine
 
User Guide: Pulsar™ Weather Station (Columbia Weather Systems)
User Guide: Pulsar™ Weather Station (Columbia Weather Systems)User Guide: Pulsar™ Weather Station (Columbia Weather Systems)
User Guide: Pulsar™ Weather Station (Columbia Weather Systems)
 
Observational constraints on mergers creating magnetism in massive stars
Observational constraints on mergers creating magnetism in massive starsObservational constraints on mergers creating magnetism in massive stars
Observational constraints on mergers creating magnetism in massive stars
 
Introduction of Human Body & Structure of cell.pptx
Introduction of Human Body & Structure of cell.pptxIntroduction of Human Body & Structure of cell.pptx
Introduction of Human Body & Structure of cell.pptx
 
Q4-Mod-1c-Quiz-Projectile-333344444.pptx
Q4-Mod-1c-Quiz-Projectile-333344444.pptxQ4-Mod-1c-Quiz-Projectile-333344444.pptx
Q4-Mod-1c-Quiz-Projectile-333344444.pptx
 
Dubai Calls Girl Lisa O525547819 Lexi Call Girls In Dubai
Dubai Calls Girl Lisa O525547819 Lexi Call Girls In DubaiDubai Calls Girl Lisa O525547819 Lexi Call Girls In Dubai
Dubai Calls Girl Lisa O525547819 Lexi Call Girls In Dubai
 
The Sensory Organs, Anatomy and Function
The Sensory Organs, Anatomy and FunctionThe Sensory Organs, Anatomy and Function
The Sensory Organs, Anatomy and Function
 
Pests of castor_Binomics_Identification_Dr.UPR.pdf
Pests of castor_Binomics_Identification_Dr.UPR.pdfPests of castor_Binomics_Identification_Dr.UPR.pdf
Pests of castor_Binomics_Identification_Dr.UPR.pdf
 
Pests of soyabean_Binomics_IdentificationDr.UPR.pdf
Pests of soyabean_Binomics_IdentificationDr.UPR.pdfPests of soyabean_Binomics_IdentificationDr.UPR.pdf
Pests of soyabean_Binomics_IdentificationDr.UPR.pdf
 
Pests of jatropha_Bionomics_identification_Dr.UPR.pdf
Pests of jatropha_Bionomics_identification_Dr.UPR.pdfPests of jatropha_Bionomics_identification_Dr.UPR.pdf
Pests of jatropha_Bionomics_identification_Dr.UPR.pdf
 
Loudspeaker- direct radiating type and horn type.pptx
Loudspeaker- direct radiating type and horn type.pptxLoudspeaker- direct radiating type and horn type.pptx
Loudspeaker- direct radiating type and horn type.pptx
 
Base editing, prime editing, Cas13 & RNA editing and organelle base editing
Base editing, prime editing, Cas13 & RNA editing and organelle base editingBase editing, prime editing, Cas13 & RNA editing and organelle base editing
Base editing, prime editing, Cas13 & RNA editing and organelle base editing
 
REVISTA DE BIOLOGIA E CIÊNCIAS DA TERRA ISSN 1519-5228 - Artigo_Bioterra_V24_...
REVISTA DE BIOLOGIA E CIÊNCIAS DA TERRA ISSN 1519-5228 - Artigo_Bioterra_V24_...REVISTA DE BIOLOGIA E CIÊNCIAS DA TERRA ISSN 1519-5228 - Artigo_Bioterra_V24_...
REVISTA DE BIOLOGIA E CIÊNCIAS DA TERRA ISSN 1519-5228 - Artigo_Bioterra_V24_...
 
Gas-ExchangeS-in-Plants-and-Animals.pptx
Gas-ExchangeS-in-Plants-and-Animals.pptxGas-ExchangeS-in-Plants-and-Animals.pptx
Gas-ExchangeS-in-Plants-and-Animals.pptx
 
CHROMATOGRAPHY PALLAVI RAWAT.pptx
CHROMATOGRAPHY  PALLAVI RAWAT.pptxCHROMATOGRAPHY  PALLAVI RAWAT.pptx
CHROMATOGRAPHY PALLAVI RAWAT.pptx
 

Presentacion Mariana Arango- biología molecular

  • 2. Is a circular non-coding RNA that acts as a tumor suppressor in pancreatic ductal adenocarcinoma (PDAC) by regulating the miR-147b/SOCS1 axis, inhibiting cell proliferation, invasion, and epithelial-mesenchymal transition (EMT). INTRODUCTION This RNA molecule plays a crucial role in the pathogenesis of PDAC and may serve as a potential therapeutic target for this aggressive cancer. CIRCTMEM-59
  • 3. This is the most lethal tumor in the world, with a five-year survival rate less than 8.5% INTRODUCTION It is the most common type of pancreatic cancer and is known for its aggressive nature and poor prognosis, often diagnosed at advanced stages PDAC
  • 4. Investigate how circular RNA circTMEM59 inhibits progression of pancreatic ductal adenocarcinoma by targeting miR-147b/SOCS1 GENERAL OBJETIVE
  • 5. METHODS Used to introduce circTMEM59 mimics, miR- 147b mimics, miR-147b inhibitors, si-SOCS1, and corresponding negative controls into PANC-1 BXPC-3 cells QRT-PCR was used to analyze gene expression in GAPDH and U6 serving as internal controls (QRT-PCR) CELL TRANSFECTION
  • 6. Used to: measure the expression of different PDAC cells when being exposed to diferent RNAs as circTMEM-59 METHODS In this assay, the luciferase gene is fused to a gene of interest, and the resulting construct is transfected into cells. WESTERN BLOTTING LUCIFERASE REPORTER ASSAY
  • 10. By understanding the molecular mechanisms underlying diseases, Doctors can now prescribe medications and therapies with greater accuracy, minimizing adverse effects and optimizing therapeutic outcomes Molecular biology has revolutionized cancer treatment by uncovering the molecular mechanisms driving tumor growth and progression. CONCLUSIONS 1 1 2
  • 11. ADENOCARCINOMA DUCTAL DE PANCREAS es investigacion Inhibe Activa Este participa en Considerado uno de los tumores mas letales CircTMEM-59 MiR-147b Este participa en SOCS-1 proliferacion invasion transicion supresion regulación de genes PDAC PDAC Se detiene la progresión del cancer En En Al inhibirse Al activarse