This document describes a study that generated synthetic spike-in mRNA-Seq data to validate fusion detection methods in cancer gene research. Researchers created artificial fusion transcripts between known cancer genes and incorporated these into cell line RNA at varying concentrations. They then sequenced the samples and analyzed the data using fusion detection tools to establish analytical parameters like sensitivity and specificity. The synthetic spike-ins allowed validation of fusion detection without real tumor samples, providing a reference for evaluating such methods in clinical and diagnostic applications of RNA-Seq.
This document describes the development and validation of a targeted next-generation sequencing (NGS) method that employs synthetic cDNA internal standards. The method uses a multiplex competitive PCR approach to amplify both native cDNA targets and known copies of synthetic internal standards, allowing measurement of each target relative to its standard. This controls for variation in library preparation and sequencing. The method was validated using ERCC RNA reference materials, demonstrating good accuracy, precision, reproducibility, and ability to detect fold-changes across platforms. Stochastic sampling effects were also evaluated, showing increased variability at low input molecules or reads. The method reduces over-sequencing needs and allows cost-effective targeted NGS analysis.
Este documento describe los fundamentos de un hogar cristiano. Resalta la importancia de crear una atmósfera pura y feliz en el hogar a través del amor, la disciplina y el buen ejemplo de los padres. También enfatiza mantener la limpieza, el orden y cultivar la virtud en los hijos para que el hogar sea un reflejo del cielo. El objetivo final es que cada familia cristiana ilustre el poder de la influencia de Cristo en el mundo.
The document describes an R package called erccdashboard that assesses technical performance in gene expression experiments using External RNA Control Consortium (ERCC) spike-in controls. The package allows users to compare results within and between laboratories. Key metrics evaluated include diagnostic accuracy, limit of detection of ratios, ratio bias, and variability. The package is demonstrated on microarray and RNA-seq data from experiments involving different laboratories and platforms.
Barton Mills Recruitment is a specialised recruitment to recruitment (rec2rec) agency with a global reach. We are one of the most established rec2rec agencies in Australia with a decade of experience under our belt. Our core business is focused on finding top recruitment talent for both agency and internal roles across the Asia Pacific Region.
The SEMisweet Side of Digital Branding (SXSW V2V)HomeAdvisor
The document discusses how HomeAdvisor rebranded from ServiceMagic and recovered from a risky domain name change through search engine optimization and paid search best practices over several years, including testing brand preferences in paid search, closely tracking performance, and focusing content and links to drive organic rankings back up after an initial 45% drop following the domain switch.
1) Natalie's fiancé of 4 years, Derek, left her for someone else, ending their engagement and leaving Natalie heartbroken in Tallahassee.
2) At a bar, Natalie notices two men, Logan and James, playing pool and checking her out. She challenges one of them, Logan, to a game of pool with the loser buying drinks.
3) Natalie impressively sinks the 8-ball on the break, winning the game and getting Logan to buy her a tequila shot. This gives Natalie a confidence boost and sense that she will be okay after her breakup.
This document describes a study that generated synthetic spike-in mRNA-Seq data to validate fusion detection methods in cancer gene research. Researchers created artificial fusion transcripts between known cancer genes and incorporated these into cell line RNA at varying concentrations. They then sequenced the samples and analyzed the data using fusion detection tools to establish analytical parameters like sensitivity and specificity. The synthetic spike-ins allowed validation of fusion detection without real tumor samples, providing a reference for evaluating such methods in clinical and diagnostic applications of RNA-Seq.
This document describes the development and validation of a targeted next-generation sequencing (NGS) method that employs synthetic cDNA internal standards. The method uses a multiplex competitive PCR approach to amplify both native cDNA targets and known copies of synthetic internal standards, allowing measurement of each target relative to its standard. This controls for variation in library preparation and sequencing. The method was validated using ERCC RNA reference materials, demonstrating good accuracy, precision, reproducibility, and ability to detect fold-changes across platforms. Stochastic sampling effects were also evaluated, showing increased variability at low input molecules or reads. The method reduces over-sequencing needs and allows cost-effective targeted NGS analysis.
Este documento describe los fundamentos de un hogar cristiano. Resalta la importancia de crear una atmósfera pura y feliz en el hogar a través del amor, la disciplina y el buen ejemplo de los padres. También enfatiza mantener la limpieza, el orden y cultivar la virtud en los hijos para que el hogar sea un reflejo del cielo. El objetivo final es que cada familia cristiana ilustre el poder de la influencia de Cristo en el mundo.
The document describes an R package called erccdashboard that assesses technical performance in gene expression experiments using External RNA Control Consortium (ERCC) spike-in controls. The package allows users to compare results within and between laboratories. Key metrics evaluated include diagnostic accuracy, limit of detection of ratios, ratio bias, and variability. The package is demonstrated on microarray and RNA-seq data from experiments involving different laboratories and platforms.
Barton Mills Recruitment is a specialised recruitment to recruitment (rec2rec) agency with a global reach. We are one of the most established rec2rec agencies in Australia with a decade of experience under our belt. Our core business is focused on finding top recruitment talent for both agency and internal roles across the Asia Pacific Region.
The SEMisweet Side of Digital Branding (SXSW V2V)HomeAdvisor
The document discusses how HomeAdvisor rebranded from ServiceMagic and recovered from a risky domain name change through search engine optimization and paid search best practices over several years, including testing brand preferences in paid search, closely tracking performance, and focusing content and links to drive organic rankings back up after an initial 45% drop following the domain switch.
1) Natalie's fiancé of 4 years, Derek, left her for someone else, ending their engagement and leaving Natalie heartbroken in Tallahassee.
2) At a bar, Natalie notices two men, Logan and James, playing pool and checking her out. She challenges one of them, Logan, to a game of pool with the loser buying drinks.
3) Natalie impressively sinks the 8-ball on the break, winning the game and getting Logan to buy her a tequila shot. This gives Natalie a confidence boost and sense that she will be okay after her breakup.
This document describes the development of a prototype synthetic microRNA mixture intended for use as a spike-in control for microRNA profiling platforms. 32 microRNAs were selected based on certain criteria and synthesized. The microRNAs were pooled and combined in varying concentrations according to a Latin Square design to generate 8 control mixtures spanning a 4-log dynamic range. Initial testing on PCR arrays showed the control mixtures generated heterologous calibration curves comparable to homogeneous controls for assessing platform performance. The synthetic microRNA control has the potential to help evaluate various performance characteristics of microRNA profiling platforms.
This document discusses the design, production, and application of Spike-In RNA Variants (SIRVs) created by Lexogen. It describes how SIRVs were modeled after natural mammalian genes and transcripts to include various transcript variants and isoforms. Their sequences were derived from viral genomes and modified to have no homology to other organisms. SIRVs were produced via in vitro transcription and tested in RNA-seq experiments mixed with other RNA controls and samples. They are intended to serve as external controls to help validate bioinformatics pipelines and the detection of transcript variants.
This document provides an agenda and summary for the ERCC 2.0 Workshop on July 10, 2014. The workshop aimed to discuss expanding the scope of the External RNA Control Consortium (ERCC) beyond version 1.0. The day included introductions, presentations from participants on applications of ERCC 1.0, and discussions on the scope and process for ERCC 2.0. Presentations summarized how ERCC 1.0 external controls were used for product development, validation of gene expression methods, and quality control of measurements. The workshop concluded with discussions on forming working groups to scope ERCC 2.0.
This document summarizes the results of five phases of testing ERCC control transcripts on Agilent microarrays to evaluate their performance. In Phase III, ERCC transcripts were spiked into background RNA at various dilutions. Analysis of probe performance at different distances from the 3' end showed good signals for probes >1000nt from the polyA tail. Phase IV constructed pools with ERCCs at different concentrations to test a wide dynamic range. Phase V experiments showed Agilent microarrays can detect small differences in ERCC levels used as barcodes to track samples. The ERCC controls helped evaluate the microarray process and different platforms.
Kate Chopin was a controversial writer in the late 1800s who wrote about women's rights and feminism. Her novel The Awakening was widely criticized for its themes of a woman rejecting traditional roles but is now recognized as an important early feminist work. Chopin used powerful and surprising endings in her stories to send messages about women's place in society and to provoke thought in her readers. Her works provided a representation of women's experiences during the time period in which she wrote.
This document discusses PacBio single molecule real-time (SMRT) sequencing of full-length cDNA transcripts. It summarizes the current challenges with transcript assembly using short-read sequencing and describes how PacBio Iso-Seq provides high-quality, full-length transcript isoforms through single-molecule long-read sequencing of cDNA. The document also reviews size selection methods like SageELF that can separate transcripts into different size fractions for sequencing.
Discovering the Best Indian Architects A Spotlight on Design Forum Internatio...Designforuminternational
India’s architectural landscape is a vibrant tapestry that weaves together the country's rich cultural heritage and its modern aspirations. From majestic historical structures to cutting-edge contemporary designs, the work of Indian architects is celebrated worldwide. Among the many firms shaping this dynamic field, Design Forum International stands out as a leader in innovative and sustainable architecture. This blog explores some of the best Indian architects, highlighting their contributions and showcasing the most famous architects in India.
Best Digital Marketing Strategy Build Your Online Presence 2024.pptxpavankumarpayexelsol
This presentation provides a comprehensive guide to the best digital marketing strategies for 2024, focusing on enhancing your online presence. Key topics include understanding and targeting your audience, building a user-friendly and mobile-responsive website, leveraging the power of social media platforms, optimizing content for search engines, and using email marketing to foster direct engagement. By adopting these strategies, you can increase brand visibility, drive traffic, generate leads, and ultimately boost sales, ensuring your business thrives in the competitive digital landscape.
This document describes the development of a prototype synthetic microRNA mixture intended for use as a spike-in control for microRNA profiling platforms. 32 microRNAs were selected based on certain criteria and synthesized. The microRNAs were pooled and combined in varying concentrations according to a Latin Square design to generate 8 control mixtures spanning a 4-log dynamic range. Initial testing on PCR arrays showed the control mixtures generated heterologous calibration curves comparable to homogeneous controls for assessing platform performance. The synthetic microRNA control has the potential to help evaluate various performance characteristics of microRNA profiling platforms.
This document discusses the design, production, and application of Spike-In RNA Variants (SIRVs) created by Lexogen. It describes how SIRVs were modeled after natural mammalian genes and transcripts to include various transcript variants and isoforms. Their sequences were derived from viral genomes and modified to have no homology to other organisms. SIRVs were produced via in vitro transcription and tested in RNA-seq experiments mixed with other RNA controls and samples. They are intended to serve as external controls to help validate bioinformatics pipelines and the detection of transcript variants.
This document provides an agenda and summary for the ERCC 2.0 Workshop on July 10, 2014. The workshop aimed to discuss expanding the scope of the External RNA Control Consortium (ERCC) beyond version 1.0. The day included introductions, presentations from participants on applications of ERCC 1.0, and discussions on the scope and process for ERCC 2.0. Presentations summarized how ERCC 1.0 external controls were used for product development, validation of gene expression methods, and quality control of measurements. The workshop concluded with discussions on forming working groups to scope ERCC 2.0.
This document summarizes the results of five phases of testing ERCC control transcripts on Agilent microarrays to evaluate their performance. In Phase III, ERCC transcripts were spiked into background RNA at various dilutions. Analysis of probe performance at different distances from the 3' end showed good signals for probes >1000nt from the polyA tail. Phase IV constructed pools with ERCCs at different concentrations to test a wide dynamic range. Phase V experiments showed Agilent microarrays can detect small differences in ERCC levels used as barcodes to track samples. The ERCC controls helped evaluate the microarray process and different platforms.
Kate Chopin was a controversial writer in the late 1800s who wrote about women's rights and feminism. Her novel The Awakening was widely criticized for its themes of a woman rejecting traditional roles but is now recognized as an important early feminist work. Chopin used powerful and surprising endings in her stories to send messages about women's place in society and to provoke thought in her readers. Her works provided a representation of women's experiences during the time period in which she wrote.
This document discusses PacBio single molecule real-time (SMRT) sequencing of full-length cDNA transcripts. It summarizes the current challenges with transcript assembly using short-read sequencing and describes how PacBio Iso-Seq provides high-quality, full-length transcript isoforms through single-molecule long-read sequencing of cDNA. The document also reviews size selection methods like SageELF that can separate transcripts into different size fractions for sequencing.
Discovering the Best Indian Architects A Spotlight on Design Forum Internatio...Designforuminternational
India’s architectural landscape is a vibrant tapestry that weaves together the country's rich cultural heritage and its modern aspirations. From majestic historical structures to cutting-edge contemporary designs, the work of Indian architects is celebrated worldwide. Among the many firms shaping this dynamic field, Design Forum International stands out as a leader in innovative and sustainable architecture. This blog explores some of the best Indian architects, highlighting their contributions and showcasing the most famous architects in India.
Best Digital Marketing Strategy Build Your Online Presence 2024.pptxpavankumarpayexelsol
This presentation provides a comprehensive guide to the best digital marketing strategies for 2024, focusing on enhancing your online presence. Key topics include understanding and targeting your audience, building a user-friendly and mobile-responsive website, leveraging the power of social media platforms, optimizing content for search engines, and using email marketing to foster direct engagement. By adopting these strategies, you can increase brand visibility, drive traffic, generate leads, and ultimately boost sales, ensuring your business thrives in the competitive digital landscape.
Practical eLearning Makeovers for EveryoneBianca Woods
Welcome to Practical eLearning Makeovers for Everyone. In this presentation, we’ll take a look at a bunch of easy-to-use visual design tips and tricks. And we’ll do this by using them to spruce up some eLearning screens that are in dire need of a new look.