SlideShare a Scribd company logo
Genomic Library
Aman Ullah
B.Sc. Med. Lab. Technology
M. Phil. Microbiology
Certificate in Health Professional Education
Lecturer, Department of Medical Lab. Technology
Institute of Paramedical Sciences, Khyber Medical
University, Peshawar, Pakistan
Introduction
A DNA library is a collection of clones of DNA designed so
that there is a high probability of finding any particular
piece of the source DNA in the collection
• There are two types of DNA library
GENOMIC LIBRARY:
• The genomic library contains DNA fragments
representing entire genome of an organism
cDNA LIBRARY:
• The cDNA library contains only complementary DNA
molecules synthesized from mRNA molecules in a cell
Genomic Library
• “A genomic library is a collection of bacteria
which have been genetically engineered to hold
the entire DNA of an organism”
• A genomic library is a collection of genes or DNA
sequences created using molecular cloning
• These libraries are constructed using clones of
bacteria or yeast that contain vectors into which
fragments of partially digested DNA have been
inserted
Genomic Library
• These bacteria and yeast are subsequently grown in
culture and when these microorganisms replicate their
genome, they also replicate the vector genome
contained within them, that is, they replicate DNA
fragments that had been inserted in vectors producing
clones of the original genome
• This collection of clones, in theory, contains all
sequences found in the original source, including the
sequence of interest
• Genomic libraries can be constructed using various
hosts like plasmids, bacteriophage lambdas and many
more
Steps of construction
• To create a human genome library, a researcher begins
by extracting and purifying DNA from human cell
• The purified DNA consist of extremely long strands
• To begin working with DNA, the strands must first be
cut into manageable sizes
• The DNA therefore is digested with restriction enzymes
which cut the DNA at specific sequences
• The restriction enzyme cut the DNA into 1000s of
smaller fragments, each of which may contain one or
more gene
Steps of construction
• Each fragment is different and have unique DNA
sequence
• To create the library, each fragment must be inserted
into loops of DNA called plasmids
• The plasmids are digested with the restriction enzymes
and then sealed to human DNA using DNA ligase
enzyme the resulting molecules are “recombinanat”
• The recombinant DNA molecule are added to bacteria,
and the bacteria are made to take up the DNA
• When bacteria have taken up the DNA, the entire
collection of cells and DNA represents a human
genome library
Uses of Genomic library
• Researchers can explore the genome of an
organism to learn more about genomic
structure and function
• They can also map the genome, identifying
the locations of specific genes
• A genomic library can also be used for the
purpose of cloning segments of DNA
Questions/Suggestions
khurramthalwi@hotamail.com

More Related Content

Similar to genomiclibrary-160531153950.pdf

Genomic and c dna library
Genomic and c dna libraryGenomic and c dna library
Genomic and c dna library
Promila Sheoran
 
Gene library
Gene libraryGene library
Gene library
Delince Samuel
 
Gene library
Gene libraryGene library
Gene library
mohanishwankhade
 
Cloning and recombinant dna
Cloning and recombinant dnaCloning and recombinant dna
Cloning and recombinant dna
Ankit Bhardwaj
 
Recombinant dna technology
Recombinant dna technologyRecombinant dna technology
Recombinant dna technology
Rahul k
 
Biotechnology and recombinant dna ch. 9
Biotechnology and recombinant dna ch. 9Biotechnology and recombinant dna ch. 9
Biotechnology and recombinant dna ch. 9
Phane Nicola M. VanderPooten
 
cloning, sudan 2016.pdf
cloning, sudan 2016.pdfcloning, sudan 2016.pdf
cloning, sudan 2016.pdf
nedalalazzwy
 
Class9 DNA technology in secondary school
Class9 DNA technology in secondary schoolClass9 DNA technology in secondary school
Class9 DNA technology in secondary school
ssusera700ad
 
genetic_enginnering_merged.pdf
genetic_enginnering_merged.pdfgenetic_enginnering_merged.pdf
genetic_enginnering_merged.pdf
tadilodessie614
 
Genomic library
Genomic libraryGenomic library
Genomic library
Chinnu S Kumar
 
Recombinant dna
Recombinant dnaRecombinant dna
Recombinant dna
Hafiz M Waseem
 
Genomic library
Genomic libraryGenomic library
Genomic library
RashidMalix
 
Construction of genomic and c dna library
Construction of genomic and c dna libraryConstruction of genomic and c dna library
Construction of genomic and c dna library
NaveenJ46
 
Genetic engineering and recombinant DNA technology (1).pptx
Genetic engineering and recombinant DNA technology (1).pptxGenetic engineering and recombinant DNA technology (1).pptx
Genetic engineering and recombinant DNA technology (1).pptx
GraceT12
 
Genomics: Organization of Genome, Strategies of Genome Sequencing, Model Plan...
Genomics: Organization of Genome, Strategies of Genome Sequencing, Model Plan...Genomics: Organization of Genome, Strategies of Genome Sequencing, Model Plan...
Genomics: Organization of Genome, Strategies of Genome Sequencing, Model Plan...
Promila Sheoran
 
Genomic library
Genomic libraryGenomic library
Genomic library
Sumit Sah
 
genomic library
genomic librarygenomic library
Gene cloning lecture notes 5 for 2010
Gene cloning lecture notes 5 for 2010Gene cloning lecture notes 5 for 2010
Gene cloning lecture notes 5 for 2010
Shadrach Meynscer
 
Biotecnología
BiotecnologíaBiotecnología
Biotecnología
Cae Upr Cayey
 
Unit 1 genetic engineering
Unit 1 genetic engineeringUnit 1 genetic engineering
Unit 1 genetic engineering
Smita Shukla
 

Similar to genomiclibrary-160531153950.pdf (20)

Genomic and c dna library
Genomic and c dna libraryGenomic and c dna library
Genomic and c dna library
 
Gene library
Gene libraryGene library
Gene library
 
Gene library
Gene libraryGene library
Gene library
 
Cloning and recombinant dna
Cloning and recombinant dnaCloning and recombinant dna
Cloning and recombinant dna
 
Recombinant dna technology
Recombinant dna technologyRecombinant dna technology
Recombinant dna technology
 
Biotechnology and recombinant dna ch. 9
Biotechnology and recombinant dna ch. 9Biotechnology and recombinant dna ch. 9
Biotechnology and recombinant dna ch. 9
 
cloning, sudan 2016.pdf
cloning, sudan 2016.pdfcloning, sudan 2016.pdf
cloning, sudan 2016.pdf
 
Class9 DNA technology in secondary school
Class9 DNA technology in secondary schoolClass9 DNA technology in secondary school
Class9 DNA technology in secondary school
 
genetic_enginnering_merged.pdf
genetic_enginnering_merged.pdfgenetic_enginnering_merged.pdf
genetic_enginnering_merged.pdf
 
Genomic library
Genomic libraryGenomic library
Genomic library
 
Recombinant dna
Recombinant dnaRecombinant dna
Recombinant dna
 
Genomic library
Genomic libraryGenomic library
Genomic library
 
Construction of genomic and c dna library
Construction of genomic and c dna libraryConstruction of genomic and c dna library
Construction of genomic and c dna library
 
Genetic engineering and recombinant DNA technology (1).pptx
Genetic engineering and recombinant DNA technology (1).pptxGenetic engineering and recombinant DNA technology (1).pptx
Genetic engineering and recombinant DNA technology (1).pptx
 
Genomics: Organization of Genome, Strategies of Genome Sequencing, Model Plan...
Genomics: Organization of Genome, Strategies of Genome Sequencing, Model Plan...Genomics: Organization of Genome, Strategies of Genome Sequencing, Model Plan...
Genomics: Organization of Genome, Strategies of Genome Sequencing, Model Plan...
 
Genomic library
Genomic libraryGenomic library
Genomic library
 
genomic library
genomic librarygenomic library
genomic library
 
Gene cloning lecture notes 5 for 2010
Gene cloning lecture notes 5 for 2010Gene cloning lecture notes 5 for 2010
Gene cloning lecture notes 5 for 2010
 
Biotecnología
BiotecnologíaBiotecnología
Biotecnología
 
Unit 1 genetic engineering
Unit 1 genetic engineeringUnit 1 genetic engineering
Unit 1 genetic engineering
 

More from sumitraDas14

pure culture notes.pdf
pure culture notes.pdfpure culture notes.pdf
pure culture notes.pdf
sumitraDas14
 
CONSTRUCTION OF GENOMIC LIBRARY MCBA P7 T (1).pdf
CONSTRUCTION OF GENOMIC LIBRARY MCBA P7 T (1).pdfCONSTRUCTION OF GENOMIC LIBRARY MCBA P7 T (1).pdf
CONSTRUCTION OF GENOMIC LIBRARY MCBA P7 T (1).pdf
sumitraDas14
 
rt-pcr-160517175331.pdf
rt-pcr-160517175331.pdfrt-pcr-160517175331.pdf
rt-pcr-160517175331.pdf
sumitraDas14
 
somaclonalvariation-130925081312-phpapp02.pdf
somaclonalvariation-130925081312-phpapp02.pdfsomaclonalvariation-130925081312-phpapp02.pdf
somaclonalvariation-130925081312-phpapp02.pdf
sumitraDas14
 
natural_sel.pptx
natural_sel.pptxnatural_sel.pptx
natural_sel.pptx
sumitraDas14
 
Genomic and cDNA Libraries.ppt
Genomic and cDNA Libraries.pptGenomic and cDNA Libraries.ppt
Genomic and cDNA Libraries.ppt
sumitraDas14
 
2011-Molecularmarker (1).ppt
2011-Molecularmarker (1).ppt2011-Molecularmarker (1).ppt
2011-Molecularmarker (1).ppt
sumitraDas14
 
IGNTU-eContent-328712472244-M.Sc-EnvironmentalScience-2-ManojkumarRai-Environ...
IGNTU-eContent-328712472244-M.Sc-EnvironmentalScience-2-ManojkumarRai-Environ...IGNTU-eContent-328712472244-M.Sc-EnvironmentalScience-2-ManojkumarRai-Environ...
IGNTU-eContent-328712472244-M.Sc-EnvironmentalScience-2-ManojkumarRai-Environ...
sumitraDas14
 
Plant Breeding.pdf
Plant Breeding.pdfPlant Breeding.pdf
Plant Breeding.pdf
sumitraDas14
 
Chromosome & nuclear structure.pdf
Chromosome & nuclear structure.pdfChromosome & nuclear structure.pdf
Chromosome & nuclear structure.pdf
sumitraDas14
 
mutation-20.2.2019.ppsx
mutation-20.2.2019.ppsxmutation-20.2.2019.ppsx
mutation-20.2.2019.ppsx
sumitraDas14
 
Bacterial-genetics.pdf
Bacterial-genetics.pdfBacterial-genetics.pdf
Bacterial-genetics.pdf
sumitraDas14
 
AFLP.doc
AFLP.docAFLP.doc
AFLP.doc
sumitraDas14
 
genetic-recombination-1 [Compatibility Mode].pdf
genetic-recombination-1 [Compatibility Mode].pdfgenetic-recombination-1 [Compatibility Mode].pdf
genetic-recombination-1 [Compatibility Mode].pdf
sumitraDas14
 
populationgrowthcurves-180429094152.pdf
populationgrowthcurves-180429094152.pdfpopulationgrowthcurves-180429094152.pdf
populationgrowthcurves-180429094152.pdf
sumitraDas14
 
rapd.ppt
rapd.pptrapd.ppt
rapd.ppt
sumitraDas14
 

More from sumitraDas14 (16)

pure culture notes.pdf
pure culture notes.pdfpure culture notes.pdf
pure culture notes.pdf
 
CONSTRUCTION OF GENOMIC LIBRARY MCBA P7 T (1).pdf
CONSTRUCTION OF GENOMIC LIBRARY MCBA P7 T (1).pdfCONSTRUCTION OF GENOMIC LIBRARY MCBA P7 T (1).pdf
CONSTRUCTION OF GENOMIC LIBRARY MCBA P7 T (1).pdf
 
rt-pcr-160517175331.pdf
rt-pcr-160517175331.pdfrt-pcr-160517175331.pdf
rt-pcr-160517175331.pdf
 
somaclonalvariation-130925081312-phpapp02.pdf
somaclonalvariation-130925081312-phpapp02.pdfsomaclonalvariation-130925081312-phpapp02.pdf
somaclonalvariation-130925081312-phpapp02.pdf
 
natural_sel.pptx
natural_sel.pptxnatural_sel.pptx
natural_sel.pptx
 
Genomic and cDNA Libraries.ppt
Genomic and cDNA Libraries.pptGenomic and cDNA Libraries.ppt
Genomic and cDNA Libraries.ppt
 
2011-Molecularmarker (1).ppt
2011-Molecularmarker (1).ppt2011-Molecularmarker (1).ppt
2011-Molecularmarker (1).ppt
 
IGNTU-eContent-328712472244-M.Sc-EnvironmentalScience-2-ManojkumarRai-Environ...
IGNTU-eContent-328712472244-M.Sc-EnvironmentalScience-2-ManojkumarRai-Environ...IGNTU-eContent-328712472244-M.Sc-EnvironmentalScience-2-ManojkumarRai-Environ...
IGNTU-eContent-328712472244-M.Sc-EnvironmentalScience-2-ManojkumarRai-Environ...
 
Plant Breeding.pdf
Plant Breeding.pdfPlant Breeding.pdf
Plant Breeding.pdf
 
Chromosome & nuclear structure.pdf
Chromosome & nuclear structure.pdfChromosome & nuclear structure.pdf
Chromosome & nuclear structure.pdf
 
mutation-20.2.2019.ppsx
mutation-20.2.2019.ppsxmutation-20.2.2019.ppsx
mutation-20.2.2019.ppsx
 
Bacterial-genetics.pdf
Bacterial-genetics.pdfBacterial-genetics.pdf
Bacterial-genetics.pdf
 
AFLP.doc
AFLP.docAFLP.doc
AFLP.doc
 
genetic-recombination-1 [Compatibility Mode].pdf
genetic-recombination-1 [Compatibility Mode].pdfgenetic-recombination-1 [Compatibility Mode].pdf
genetic-recombination-1 [Compatibility Mode].pdf
 
populationgrowthcurves-180429094152.pdf
populationgrowthcurves-180429094152.pdfpopulationgrowthcurves-180429094152.pdf
populationgrowthcurves-180429094152.pdf
 
rapd.ppt
rapd.pptrapd.ppt
rapd.ppt
 

Recently uploaded

Présentationvvvvvvvvvvvvvvvvvvvvvvvvvvvv2.pptx
Présentationvvvvvvvvvvvvvvvvvvvvvvvvvvvv2.pptxPrésentationvvvvvvvvvvvvvvvvvvvvvvvvvvvv2.pptx
Présentationvvvvvvvvvvvvvvvvvvvvvvvvvvvv2.pptx
siemaillard
 
Beyond Degrees - Empowering the Workforce in the Context of Skills-First.pptx
Beyond Degrees - Empowering the Workforce in the Context of Skills-First.pptxBeyond Degrees - Empowering the Workforce in the Context of Skills-First.pptx
Beyond Degrees - Empowering the Workforce in the Context of Skills-First.pptx
EduSkills OECD
 
spot a liar (Haiqa 146).pptx Technical writhing and presentation skills
spot a liar (Haiqa 146).pptx Technical writhing and presentation skillsspot a liar (Haiqa 146).pptx Technical writhing and presentation skills
spot a liar (Haiqa 146).pptx Technical writhing and presentation skills
haiqairshad
 
مصحف القراءات العشر أعد أحرف الخلاف سمير بسيوني.pdf
مصحف القراءات العشر   أعد أحرف الخلاف سمير بسيوني.pdfمصحف القراءات العشر   أعد أحرف الخلاف سمير بسيوني.pdf
مصحف القراءات العشر أعد أحرف الخلاف سمير بسيوني.pdf
سمير بسيوني
 
How to deliver Powerpoint Presentations.pptx
How to deliver Powerpoint  Presentations.pptxHow to deliver Powerpoint  Presentations.pptx
How to deliver Powerpoint Presentations.pptx
HajraNaeem15
 
BÀI TẬP DẠY THÊM TIẾNG ANH LỚP 7 CẢ NĂM FRIENDS PLUS SÁCH CHÂN TRỜI SÁNG TẠO ...
BÀI TẬP DẠY THÊM TIẾNG ANH LỚP 7 CẢ NĂM FRIENDS PLUS SÁCH CHÂN TRỜI SÁNG TẠO ...BÀI TẬP DẠY THÊM TIẾNG ANH LỚP 7 CẢ NĂM FRIENDS PLUS SÁCH CHÂN TRỜI SÁNG TẠO ...
BÀI TẬP DẠY THÊM TIẾNG ANH LỚP 7 CẢ NĂM FRIENDS PLUS SÁCH CHÂN TRỜI SÁNG TẠO ...
Nguyen Thanh Tu Collection
 
Temple of Asclepius in Thrace. Excavation results
Temple of Asclepius in Thrace. Excavation resultsTemple of Asclepius in Thrace. Excavation results
Temple of Asclepius in Thrace. Excavation results
Krassimira Luka
 
REASIGNACION 2024 UGEL CHUPACA 2024 UGEL CHUPACA.pdf
REASIGNACION 2024 UGEL CHUPACA 2024 UGEL CHUPACA.pdfREASIGNACION 2024 UGEL CHUPACA 2024 UGEL CHUPACA.pdf
REASIGNACION 2024 UGEL CHUPACA 2024 UGEL CHUPACA.pdf
giancarloi8888
 
Walmart Business+ and Spark Good for Nonprofits.pdf
Walmart Business+ and Spark Good for Nonprofits.pdfWalmart Business+ and Spark Good for Nonprofits.pdf
Walmart Business+ and Spark Good for Nonprofits.pdf
TechSoup
 
Stack Memory Organization of 8086 Microprocessor
Stack Memory Organization of 8086 MicroprocessorStack Memory Organization of 8086 Microprocessor
Stack Memory Organization of 8086 Microprocessor
JomonJoseph58
 
Leveraging Generative AI to Drive Nonprofit Innovation
Leveraging Generative AI to Drive Nonprofit InnovationLeveraging Generative AI to Drive Nonprofit Innovation
Leveraging Generative AI to Drive Nonprofit Innovation
TechSoup
 
Jemison, MacLaughlin, and Majumder "Broadening Pathways for Editors and Authors"
Jemison, MacLaughlin, and Majumder "Broadening Pathways for Editors and Authors"Jemison, MacLaughlin, and Majumder "Broadening Pathways for Editors and Authors"
Jemison, MacLaughlin, and Majumder "Broadening Pathways for Editors and Authors"
National Information Standards Organization (NISO)
 
Lifelines of National Economy chapter for Class 10 STUDY MATERIAL PDF
Lifelines of National Economy chapter for Class 10 STUDY MATERIAL PDFLifelines of National Economy chapter for Class 10 STUDY MATERIAL PDF
Lifelines of National Economy chapter for Class 10 STUDY MATERIAL PDF
Vivekanand Anglo Vedic Academy
 
RHEOLOGY Physical pharmaceutics-II notes for B.pharm 4th sem students
RHEOLOGY Physical pharmaceutics-II notes for B.pharm 4th sem studentsRHEOLOGY Physical pharmaceutics-II notes for B.pharm 4th sem students
RHEOLOGY Physical pharmaceutics-II notes for B.pharm 4th sem students
Himanshu Rai
 
Juneteenth Freedom Day 2024 David Douglas School District
Juneteenth Freedom Day 2024 David Douglas School DistrictJuneteenth Freedom Day 2024 David Douglas School District
Juneteenth Freedom Day 2024 David Douglas School District
David Douglas School District
 
C1 Rubenstein AP HuG xxxxxxxxxxxxxx.pptx
C1 Rubenstein AP HuG xxxxxxxxxxxxxx.pptxC1 Rubenstein AP HuG xxxxxxxxxxxxxx.pptx
C1 Rubenstein AP HuG xxxxxxxxxxxxxx.pptx
mulvey2
 
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
GeorgeMilliken2
 
Philippine Edukasyong Pantahanan at Pangkabuhayan (EPP) Curriculum
Philippine Edukasyong Pantahanan at Pangkabuhayan (EPP) CurriculumPhilippine Edukasyong Pantahanan at Pangkabuhayan (EPP) Curriculum
Philippine Edukasyong Pantahanan at Pangkabuhayan (EPP) Curriculum
MJDuyan
 
Pengantar Penggunaan Flutter - Dart programming language1.pptx
Pengantar Penggunaan Flutter - Dart programming language1.pptxPengantar Penggunaan Flutter - Dart programming language1.pptx
Pengantar Penggunaan Flutter - Dart programming language1.pptx
Fajar Baskoro
 
BBR 2024 Summer Sessions Interview Training
BBR  2024 Summer Sessions Interview TrainingBBR  2024 Summer Sessions Interview Training
BBR 2024 Summer Sessions Interview Training
Katrina Pritchard
 

Recently uploaded (20)

Présentationvvvvvvvvvvvvvvvvvvvvvvvvvvvv2.pptx
Présentationvvvvvvvvvvvvvvvvvvvvvvvvvvvv2.pptxPrésentationvvvvvvvvvvvvvvvvvvvvvvvvvvvv2.pptx
Présentationvvvvvvvvvvvvvvvvvvvvvvvvvvvv2.pptx
 
Beyond Degrees - Empowering the Workforce in the Context of Skills-First.pptx
Beyond Degrees - Empowering the Workforce in the Context of Skills-First.pptxBeyond Degrees - Empowering the Workforce in the Context of Skills-First.pptx
Beyond Degrees - Empowering the Workforce in the Context of Skills-First.pptx
 
spot a liar (Haiqa 146).pptx Technical writhing and presentation skills
spot a liar (Haiqa 146).pptx Technical writhing and presentation skillsspot a liar (Haiqa 146).pptx Technical writhing and presentation skills
spot a liar (Haiqa 146).pptx Technical writhing and presentation skills
 
مصحف القراءات العشر أعد أحرف الخلاف سمير بسيوني.pdf
مصحف القراءات العشر   أعد أحرف الخلاف سمير بسيوني.pdfمصحف القراءات العشر   أعد أحرف الخلاف سمير بسيوني.pdf
مصحف القراءات العشر أعد أحرف الخلاف سمير بسيوني.pdf
 
How to deliver Powerpoint Presentations.pptx
How to deliver Powerpoint  Presentations.pptxHow to deliver Powerpoint  Presentations.pptx
How to deliver Powerpoint Presentations.pptx
 
BÀI TẬP DẠY THÊM TIẾNG ANH LỚP 7 CẢ NĂM FRIENDS PLUS SÁCH CHÂN TRỜI SÁNG TẠO ...
BÀI TẬP DẠY THÊM TIẾNG ANH LỚP 7 CẢ NĂM FRIENDS PLUS SÁCH CHÂN TRỜI SÁNG TẠO ...BÀI TẬP DẠY THÊM TIẾNG ANH LỚP 7 CẢ NĂM FRIENDS PLUS SÁCH CHÂN TRỜI SÁNG TẠO ...
BÀI TẬP DẠY THÊM TIẾNG ANH LỚP 7 CẢ NĂM FRIENDS PLUS SÁCH CHÂN TRỜI SÁNG TẠO ...
 
Temple of Asclepius in Thrace. Excavation results
Temple of Asclepius in Thrace. Excavation resultsTemple of Asclepius in Thrace. Excavation results
Temple of Asclepius in Thrace. Excavation results
 
REASIGNACION 2024 UGEL CHUPACA 2024 UGEL CHUPACA.pdf
REASIGNACION 2024 UGEL CHUPACA 2024 UGEL CHUPACA.pdfREASIGNACION 2024 UGEL CHUPACA 2024 UGEL CHUPACA.pdf
REASIGNACION 2024 UGEL CHUPACA 2024 UGEL CHUPACA.pdf
 
Walmart Business+ and Spark Good for Nonprofits.pdf
Walmart Business+ and Spark Good for Nonprofits.pdfWalmart Business+ and Spark Good for Nonprofits.pdf
Walmart Business+ and Spark Good for Nonprofits.pdf
 
Stack Memory Organization of 8086 Microprocessor
Stack Memory Organization of 8086 MicroprocessorStack Memory Organization of 8086 Microprocessor
Stack Memory Organization of 8086 Microprocessor
 
Leveraging Generative AI to Drive Nonprofit Innovation
Leveraging Generative AI to Drive Nonprofit InnovationLeveraging Generative AI to Drive Nonprofit Innovation
Leveraging Generative AI to Drive Nonprofit Innovation
 
Jemison, MacLaughlin, and Majumder "Broadening Pathways for Editors and Authors"
Jemison, MacLaughlin, and Majumder "Broadening Pathways for Editors and Authors"Jemison, MacLaughlin, and Majumder "Broadening Pathways for Editors and Authors"
Jemison, MacLaughlin, and Majumder "Broadening Pathways for Editors and Authors"
 
Lifelines of National Economy chapter for Class 10 STUDY MATERIAL PDF
Lifelines of National Economy chapter for Class 10 STUDY MATERIAL PDFLifelines of National Economy chapter for Class 10 STUDY MATERIAL PDF
Lifelines of National Economy chapter for Class 10 STUDY MATERIAL PDF
 
RHEOLOGY Physical pharmaceutics-II notes for B.pharm 4th sem students
RHEOLOGY Physical pharmaceutics-II notes for B.pharm 4th sem studentsRHEOLOGY Physical pharmaceutics-II notes for B.pharm 4th sem students
RHEOLOGY Physical pharmaceutics-II notes for B.pharm 4th sem students
 
Juneteenth Freedom Day 2024 David Douglas School District
Juneteenth Freedom Day 2024 David Douglas School DistrictJuneteenth Freedom Day 2024 David Douglas School District
Juneteenth Freedom Day 2024 David Douglas School District
 
C1 Rubenstein AP HuG xxxxxxxxxxxxxx.pptx
C1 Rubenstein AP HuG xxxxxxxxxxxxxx.pptxC1 Rubenstein AP HuG xxxxxxxxxxxxxx.pptx
C1 Rubenstein AP HuG xxxxxxxxxxxxxx.pptx
 
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
 
Philippine Edukasyong Pantahanan at Pangkabuhayan (EPP) Curriculum
Philippine Edukasyong Pantahanan at Pangkabuhayan (EPP) CurriculumPhilippine Edukasyong Pantahanan at Pangkabuhayan (EPP) Curriculum
Philippine Edukasyong Pantahanan at Pangkabuhayan (EPP) Curriculum
 
Pengantar Penggunaan Flutter - Dart programming language1.pptx
Pengantar Penggunaan Flutter - Dart programming language1.pptxPengantar Penggunaan Flutter - Dart programming language1.pptx
Pengantar Penggunaan Flutter - Dart programming language1.pptx
 
BBR 2024 Summer Sessions Interview Training
BBR  2024 Summer Sessions Interview TrainingBBR  2024 Summer Sessions Interview Training
BBR 2024 Summer Sessions Interview Training
 

genomiclibrary-160531153950.pdf

  • 1. Genomic Library Aman Ullah B.Sc. Med. Lab. Technology M. Phil. Microbiology Certificate in Health Professional Education Lecturer, Department of Medical Lab. Technology Institute of Paramedical Sciences, Khyber Medical University, Peshawar, Pakistan
  • 2. Introduction A DNA library is a collection of clones of DNA designed so that there is a high probability of finding any particular piece of the source DNA in the collection • There are two types of DNA library GENOMIC LIBRARY: • The genomic library contains DNA fragments representing entire genome of an organism cDNA LIBRARY: • The cDNA library contains only complementary DNA molecules synthesized from mRNA molecules in a cell
  • 3. Genomic Library • “A genomic library is a collection of bacteria which have been genetically engineered to hold the entire DNA of an organism” • A genomic library is a collection of genes or DNA sequences created using molecular cloning • These libraries are constructed using clones of bacteria or yeast that contain vectors into which fragments of partially digested DNA have been inserted
  • 4. Genomic Library • These bacteria and yeast are subsequently grown in culture and when these microorganisms replicate their genome, they also replicate the vector genome contained within them, that is, they replicate DNA fragments that had been inserted in vectors producing clones of the original genome • This collection of clones, in theory, contains all sequences found in the original source, including the sequence of interest • Genomic libraries can be constructed using various hosts like plasmids, bacteriophage lambdas and many more
  • 5. Steps of construction • To create a human genome library, a researcher begins by extracting and purifying DNA from human cell • The purified DNA consist of extremely long strands • To begin working with DNA, the strands must first be cut into manageable sizes • The DNA therefore is digested with restriction enzymes which cut the DNA at specific sequences • The restriction enzyme cut the DNA into 1000s of smaller fragments, each of which may contain one or more gene
  • 6. Steps of construction • Each fragment is different and have unique DNA sequence • To create the library, each fragment must be inserted into loops of DNA called plasmids • The plasmids are digested with the restriction enzymes and then sealed to human DNA using DNA ligase enzyme the resulting molecules are “recombinanat” • The recombinant DNA molecule are added to bacteria, and the bacteria are made to take up the DNA • When bacteria have taken up the DNA, the entire collection of cells and DNA represents a human genome library
  • 7.
  • 8. Uses of Genomic library • Researchers can explore the genome of an organism to learn more about genomic structure and function • They can also map the genome, identifying the locations of specific genes • A genomic library can also be used for the purpose of cloning segments of DNA